scholarly journals Diaporthe species infecting sunflower in Russia

2020 ◽  
Vol 18 ◽  
pp. 00010
Author(s):  
Maria Gomzhina ◽  
Philipp Gannibal
Keyword(s):  
Morphological Analysis ◽  
Plant Protection ◽  
Morphological Characteristics ◽  
Pathogenic Fungi ◽  
Molecular Techniques ◽  
Diaporthe Phaseolorum ◽  
Early Classification ◽  
Molecular Phylogenetic ◽  
Type Data ◽  
First Time

Diaporthe is an important group of plant pathogenic fungi revealed all over the world. Early classification and species identification of this genus was mostly based on combination of morphological characteristics, cultural features, and affiliation with a host plant. According to recent investigations, valid distinction between Diaporthe species should have combined molecular techniques, morphological and cultural observations, and mating type data. In Russia a comprehensive and extensive analysis of biodiversity and geographic distribution of Diaporthe species infecting sunflower has not been performed. There were seven Diaporthe sp. strains isolated from this plant maintained in the Laboratory of Mycology and Phytopathology of All-Russian Institute of Plant Protection. In previous study a strain from Krasnodar region, based on combination of molecular and morphological features was identified as Diaporthe phaseolorum. The aim of this study was to identify all other strains using primarily molecular phylogenetic approach and traditional morphological analysis. The strains were identified as Diaporthe gulyae, Diaporthe eres, and Diaporthe helianthi. Two species - D. gulyae and D. eres are found for the first time on sunflower in Russia. Detection of D. helianthi is the first report of this fungus in Russia as confirmed by molecular analysis.

scholarly journals Two newly recorded invasive alien ascidians (Chordata, Tunicata, Ascidiacea) based on morphological and molecular phylogenetic analysis in Korea

Zootaxa ◽  
2012 ◽  
Vol 3368 (1) ◽  
pp. 211 ◽  
Author(s):  
JOOYEON PYO ◽  
TAEKJUN LEE ◽  
SOOK SHIN
Keyword(s):  
Invasive Species ◽  
Phylogenetic Analysis ◽  
Molecular Analysis ◽  
Phylogenetic Relationships ◽  
18S Rdna ◽  
Morphological Characteristics ◽  
Korea Strait ◽  
Molecular Phylogenetic ◽  
Distinct Features ◽  
First Time

We report five alien ascidians with some distinct features that were investigated from August 2009 to October 2011 in Korea, among which Ascidiella aspersa (Müller, 1776) belonging to family Ascidiidae of order Phlebobranchia and Molgula manhattensis (De Kay, 1843) belonging to family Molgulidae of order Stolidobranchia are newly discovered invasive species. These ascidians were identified and their phylogenetic relationships were clarified through molecular analysis using about 680 bp of nuclear 18S rDNA and about 670 bp of mt-COI genes along with detailed morphological characteristics, and reported for the first time in Korea. It was discovered that A. aspersa was widespread three coastlines of Korea except Jeju Island, and M. manhattensis first found in Mokpo, Gunsan, and Incheon in June 2010 extended into Busan of Korea Strait in 2011.

scholarly journals Morphological study of fruits, seeds and embryo in the tropical tribe Dipterygeae (Leguminosae-Papilionoideae)

Rodriguésia ◽  
2014 ◽  
Vol 65 (1) ◽  
pp. 89-97 ◽  
Author(s):  
Rafael Barbosa Pinto ◽  
Vanessa Maria da Costa Rodrigues Francisco ◽  
Vidal de Freitas Mansano
Keyword(s):  
Morphological Study ◽  
Morphological Analysis ◽  
Morphological Characteristics ◽  
The Other ◽  
Embryo Morphology ◽  
Phylogenetic Studies ◽  
Dispersal Syndromes ◽  
Diagnostic Characteristics ◽  
First Time

This work analyses and describes fruit, seed and embryo morphology of 12 Dipterygeae species. The fruit is quite distinct among genera, being a legume in Taralea, a drupoid legume in Dipteryx and a cryptosamara in Pterodon. It was observed that the three genera present distinct morphological characteristics in the epicarp, mesocarp and endocarp. These structures are strongly associated with the dispersal syndromes presented by the genera. Embryonic descriptions are provided for the first time in this manuscript for the three genera presented here and the main diagnostic characteristics are: (1) a gradual extent of development of the hypocotyl-radicle axis from Taralea (undeveloped) to Dipteryx (the most developed); (2) the plumules are entire in Taralea and pinnate in Dipteryx and Pterodon; (3) a cleft below the hypocotyl-radicle axis in Taralea, absent in the other genera, because of the strongly cordate base of the cotyledonar leaves; (4) differences in the degree of development of the hypocotyl-radicle axis between D.alata, D.rosea and D.micrantha. Dipterygeae presents a noticeable diversity of fruit, seed and embryo, Taralea being the most distinct of all. The results of this morphological analysis suggest an evolutive path for the diaspores in the clade, based on phylogenetic studies which show Taralea as sister to the clade constituted by Dipteryx and Pterodon.

scholarly journals First Report of Cochliobolus sativus on Guzmania sp. in Belgium

Plant Disease ◽  
2006 ◽  
Vol 90 (10) ◽  
pp. 1361-1361 ◽  
Author(s):  
C. Crepel ◽  
S. Inghelbrecht ◽  
S. Baeyen ◽  
M. Maes
Keyword(s):  
Morphological Characteristics ◽  
Aerial Mycelium ◽  
Pathogenic Fungi ◽  
Bipolaris Sorokiniana ◽  
Molecular Techniques ◽  
Cochliobolus Sativus ◽  
Fungal Mycelium ◽  
Distilled Water ◽  
Its1 Sequence ◽  
Leaf Spots

In 2001 and again in December 2005, an outbreak of leaf spots was observed on Guzmania sp. ‘Gwendolyne’ (Bromeliaceae) in a Belgian nursery. Typical disease symptoms were irregular spots with a grayish center and a narrow red-brown margin. Identification was based on morphological characteristics and molecular techniques. Isolations of diseased leaf tissues previously washed with sterile distilled water on potato dextrose agar (PDA) resulted in mycelial colonies after 7 to 8 days. Fungal mycelium grew at a linear rate of 30.4 mm per 24 h at 21°C in the dark. The pathogen produced aerial mycelium and sporulation was abundant. The color of the colonies on PDA was pale to dark brown and conidial characteristics similar to those of Cochliobolus sativus (anamorph Bipolaris sorokiniana) (1) were observed: brown ellipsoidal spores rounded at the top, 3 to 12 distoseptate, with average dimensions of 40 to 120 × 17 to 28 μm. The pathogen was also characterized with molecular tools. DNA was isolated from mycelium from a PDA plate. The ribosomal DNA region ITS1-5.8S-ITS2 was amplified and cloned. The ITS1 sequences (174 bp) of two independent clones were analyzed. The three highest similarity scores (E = 2e-71) obtained in BLAST were C. sativus (GenBank Accession Nos. AF158105 and AF071329) and B. sorokiniana strain BS11 (GenBank Accession No. AY372677). For these, pairwise alignments resulted in an identical score of 97.1% (169 identical bases, four indels, and one transversion). The new Genbank Accession No. of the ITS1 sequence is DQ 641269. To prove pathogenicity of the isolate, inoculations were done by spraying leaves of three young Guzmania sp. ‘Gwendolyne’ plants with a 20-ml spore suspension (106 spores/ml). Three plants were sprayed with sterile distilled water as controls. The plants were kept for 48 h under a humid chamber and subsequently at room temperature (20 to 25°C) on the laboratory bench. Three to four days after inoculation, leaf spots were observed and C. sativus (anamorph B. sorokiniana) was reisolated, completing Koch's postulates successfully. On the basis of symptoms, morphological characteristics, and pathogenicity tests, the pathogen was identified as C. sativus (anamorph B. sorokiniana). To our knowledge, this is the first record of C. sativus (anamorph B. sorokiniana) on Guzmania sp. in Belgium. References: (1) A. Sivanesan and P. Holliday. Description of Pathogenic Fungi and Bacteria. CMI, Kew, Surrey, England, UK, 1981.

scholarly journals A survey of subtropical nursery plants for fungal diseases in Northland

2006 ◽  
Vol 59 ◽  
pp. 132-136 ◽  
Author(s):  
M. Braithwaite ◽  
C.F. Hill ◽  
S. Ganev ◽  
J.M. Pay ◽  
H.G. Pearson ◽  
...  
Keyword(s):  
New Zealand ◽  
Plant Pathogens ◽  
Morphological Characteristics ◽  
Molecular Techniques ◽  
Plant Diseases ◽  
New Host ◽  
Disease Symptoms ◽  
Plant Families ◽  
First Time

During 2003 and 2004 fortyfive randomly selected wholesale and retail plant nurseries were surveyed for plant diseases The plant families Agavaceae Annonaceae Arecaceae Bromeliaceae Cycadaceae and Musaceae were targeted Plants were examined in situ for disease symptoms as well as samples being collected for laboratory analyses Fungi were identified using morphological characteristics and where necessary with molecular techniques The survey resulted in a range of fungi being identified from the target plants These fungi ranged from saprophytes to plant pathogens some of which may have undesirable effects on New Zealands biodiversity or economy Many new host/pathogen records were observed and several fungi were detected for the first time in New Zealand This paper presents and discusses the results of these findings

New species of Camptophora and Cyphellophora from China, and first report of sexual morphs for these genera

Phytotaxa ◽  
2018 ◽  
Vol 343 (2) ◽  
pp. 149 ◽  
Author(s):  
HUI YANG ◽  
KEVIN D. HYDE ◽  
SAMANTHA C. KARUNARATHNA ◽  
CHAO DENG ◽  
CHANG-HUA GU ◽  
...  
Keyword(s):  
New Species ◽  
Yunnan Province ◽  
New Genus ◽  
Sequence Data ◽  
Morphological Characteristics ◽  
Plant Materials ◽  
Molecular Phylogenetic ◽  
Phylogenetic Lineages ◽  
First Time ◽  
Asexual Morphs

The genus Cyphellophora have been reported from plant materials, mammal’s skins and nails, and food. Previous research studies have only provided morphological characteristics of the asexual morphs of this genus. The genus Camptophora was introduced as a new genus segregated from Cyphellophora based on the sickle-shaped septate conidia and phylogenetic lineages. For the first time, we provide morphological characteristics of a sexual morph of Cyphellophora and Camptophora collected and isolated in Yunnan Province of China. Cyphellophora jingdongensis and Camptophora schima are introduced as new species while Ca. hylomeconis is reported as a new record to China based on both morphological characteristics and molecular phylogenetic analysis with combined ITS and LSU sequence data, and are compared with related taxa. Full descriptions, illustrations, colour photographs and notes are provided for three species.

scholarly journals Grappling with homoplasy: taxonomic refinements and reassignments in the ant genera Camponotus and Colobopsis (Hymenoptera: Formicidae)

2021 ◽  
Vol 79 ◽  
pp. 37-56
Author(s):  
Philip S. Ward ◽  
Brendon E. Boudinot
Keyword(s):  
Type Species ◽  
Morphological Analysis ◽  
Distinct Species ◽  
Species Level ◽  
Phylogenetic Studies ◽  
Senior Synonym ◽  
Molecular Phylogenetic ◽  
Male Morphology ◽  
First Time ◽  
Lines Of Evidence

Camponotus and Colobopsis are widely distributed and species-rich genera in the ant tribe Camponotini. Molecular phylogenetic studies demonstrate that they are not sister taxa, but several lineages within each genus have converged to a remarkable degree, confounding the taxonomy of these ants. Based on multiple lines of evidence, including worker and male morphology, we demonstrate that: (1) three species of “Camponotus” belonging to the subgenus Myrmotemnus, including its type species, are in fact members of the genus Colobopsis; (2) four species previously assigned to Colobopsis belong to the subgenus Myrmamblys of Camponotus; and (3) three Nearctic taxa recently placed in Colobopsis are members of the genus Camponotus and closely related to Camponotus clarithorax. These taxonomic findings yield the following new or revived combinations: Colobopsis moeschi (comb. nov.), Colobopsis moeschi lygaea (comb. nov.), Colobopsis nutans (comb. nov.), Colobopsis nutans cleliae (comb. nov.), and Colobopsis reichenspergeri (comb. nov.); Camponotus apostemata (comb. nov.), Camponotus aurelianus (comb. rev.), Camponotus cavibregma (comb. nov.), Camponotus horrens (comb. rev.), Camponotus politae (comb. rev.), Camponotus trajanus (comb. rev.), and Camponotus yogi (comb. rev.). A further consequence is the following generic synonymy (senior synonym listed first): Colobopsis = Myrmotemnussyn. nov., and Camponotus = Dolophrasyn. rev. At the species level, we argue that Camponotus apostemata and Camponotus cavibregma are junior synonyms (syn. nov.) of Camponotus yogi, and Camponotus quercicola is a junior synonym (syn. nov.) of Ca. laevigatus. Taxonomic comments are also provided on some members of the Camponotus reticulatus group, with Camponotus adustus (stat. nov.) and Ca. leucodiscus (stat. rev.) being recognized as distinct species rather than subspecies of Ca. bellus. A male-based diagnosis of the Camponotini is provided, and differences between the males of Colobopsis and Camponotus are documented and illustrated for the first time. This study reveals new character systems of potential value to the systematics of these ants, including features of the male genitalia, and emphasizes the value of reciprocal illumination between phylogenomics and critical morphological analysis.

Rediscovery of Dacrymyces pezizoides (Dacrymycetes, Basidiomycota) 80 years after its original description

Phytotaxa ◽  
2018 ◽  
Vol 371 (5) ◽  
pp. 293 ◽  
Author(s):  
TAKASHI SHIROUZU ◽  
KOJIN SANJO
Keyword(s):  
Phylogenetic Analysis ◽  
Morphological Characteristics ◽  
Original Description ◽  
Fruiting Bodies ◽  
Its Regions ◽  
Molecular Phylogenetic ◽  
The Dead ◽  
First Time ◽  
Warm Temperate ◽  
Lsu Nrdna

Dacrymyces pezizoides has been rediscovered for the first time since its original description in 1939. Dacrymycetous fruiting bodies recently collected from the dead branches of broad-leaved trees in Japan were identified as D. pezizoides based on their morphological characteristics, i.e., turbinate or discoid basidiocarps, hyphae without clamp connections, thick-walled marginal hyphae, and 3-septate basidiospores. Molecular phylogenetic analysis based on the LSU nrDNA and ITS regions revealed that D. pezizoides forms a clade with other Dacrymyces spp., such as D. stillatus and D. chrysospermus, and Guepiniopsis buccina. Dacrymyces pezizoides is considered to form basidiocarps in autumn on the dead branches of broad-leaved trees in warm-temperate areas of Japan.

scholarly journals New species and records of Chapsa (Graphidaceae) in China

MycoKeys ◽  
2021 ◽  
Vol 85 ◽  
pp. 73-85
Author(s):  
Ming-Zhu Dou ◽  
Min Li ◽  
Ze-Feng Jia
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Molecular Phylogenetic Analysis ◽  
Phenotypic Traits ◽  
Molecular Phylogenetic ◽  
Chemical Traits ◽  
First Time

We studied the genus Chapsa in China based on morphological characteristics, chemical traits and molecular phylogenetic analysis. One species new to science (C. murioelongata M.Z. Dou & M. Li) and two records new to China were found (C. wolseleyana Weerakoon, Lumbsch & Lücking and C. niveocarpa Mangold). Chapsa murioelongatasp. nov. is characterised by its lobed thalline margin, orange discs with white pruina, clear hymenium, and submuriform and long ascospores. Chapsa wolseleyana was recombined into Astrochapsa based on phenotypic traits. Sequences of this species are for the first time reported here and phylogenetic analyses of three loci (mtSSU, ITS and nuLSU) supported the position of this species within Chapsa. A key for the Chapsa species known in China is provided.

Cas de suie de l'érable et de chancre du peuplier dans le canton de Genève

2016 ◽  
Vol 167 (2) ◽  
pp. 98-104
Author(s):  
Bastien Cochard ◽  
François Lefort
Keyword(s):  
Internal Transcribed Spacer ◽  
Pathogenic Fungi ◽  
Fungal Flora ◽  
Acer Campestre ◽  
Cytospora Chrysosperma ◽  
Pure Cultures ◽  
Populus X Euramericana ◽  
Bark Peeling ◽  
Specific Symptoms ◽  
First Time

A case of sooty bark disease and Cytospora poplar canker in the Canton of Geneva In summer 2014, a case of sooty bark disease caused by Cryptostroma corticale on an individual field maple (Acer campestre) and two cases of poplar canker due to Cytospora chrysosperma on Populus x euramericana were identified genetically for the first time on the territory of the Canton of Geneva. In both cases, the trees presented signs of very advanced dieback, accompanied by specific symptoms such as bark peeling and sooty plaques for the maple, and loose twisted bark layers and black colouring of the wood in structural branches of the poplars. Sampling was carried out in the symptomatic areas and components of the fungal flora were isolated in pure cultures in order to identify any pathogenic fungi. The molecular analysis of the rDNA internal transcribed spacer (ITS) sequences made it possible to identify precisely all pure isolates obtained. The results showed a majority presence of C. corticale in the maple tree, and of C. chrysosperma in the two poplars. Both these fungi are little known in Switzerland and Europe, and their presence is maybe associated with changes in the climate.

First record of Pestalotiopsis spp. from affected leaves of mastic shrubs (Pestacia lentiscus L.) in northeastern of Libya.

2016 ◽  
Vol 5 (08) ◽  
pp. 4744
Author(s):  
Zahra Ibrahim El-Gali
Keyword(s):  
Signs And Symptoms ◽  
Pathogenic Fungi ◽  
First Record ◽  
Plant Pathogenic Fungi ◽  
Pistacia Lentiscus ◽  
Plant Materials ◽  
Al Jabal Al Akhdar ◽  
First Time

This study was carried out to identify the unknown different symptoms and their causes as plant pathogenic fungi from Al-Jabal Al-Akhdar District. Plant materials with fungal signs and symptoms were collected and examined. The main fungi consistently isolated from symptomatic leaves and twigs were Pestalotiopsis spp. Morphology, colony characteristics, and pathogenicity of the isolates were examined. My report the occurrence of Pestalotiopsis spp. on leaves of mastic (Pistacia lentiscus) for the first time in Libya.

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