Variety study of black currant plants (Ribes nigrum L.) of Bashkir breeding obtained by clonal micropropagation in vitro

2020 ◽  
Vol 60 (1) ◽  
pp. 36-41
Author(s):  
L. A. Golovina ◽  
R. A. Nigmatzyanov
Keyword(s):  
Ribes Nigrum ◽  
Black Currant ◽  
Clonal Micropropagation

In vitro rooting cultivars of black currant (Ribes nigrum L.) of the Bashkir breeding

Biomics ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 332-335 ◽  
Author(s):  
L.A. Golovina ◽  
M.M. Ishmuratova
Keyword(s):  
In Vitro Rooting ◽  
Ribes Nigrum ◽  
Black Currant

scholarly journals Influence of climatic conditions of the introduction period and varietal characteristics of black currant (Ribes nigrum L.) on the effectiveness of culture initiation in vitro

2021 ◽  
Vol 36 ◽  
pp. 04010
Author(s):  
T.M. Khromova ◽  
L.V. Tashmatova ◽  
O.V. Matsneva ◽  
V.V. Shakhov
Keyword(s):  
Survival Rate ◽  
Physiological State ◽  
Climatic Conditions ◽  
Source Material ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Active Growth ◽  
Murashige Skoog ◽  
One Year

The article presents data from the effectiveness studies of the initial introduction stage of black currant (Ribes nigrum L.) into in vitro culture depending on the introduction period and the corresponding climatic conditions. The research objects were varieties of black currants selected by the Russian Research Institute of Fruit Crop Breeding: Azhurnaya, Orlovskaya serenada, Ocharovanie, Chudnoye mgnovenye. The introduction into in vitro culture was carried out in several periods characterized by different physiological states of the explants: the period of dormancy release (mid-March), the period of active growth (June), and the period of growth decay (mid-September). The source material in the spring and autumn periods were the buds of one-year stiffened shoots, in the summer introduction period - the buds of growing green shoots. The cultivation was carried out on Murashige-Skoog medium supplemented with 6-BAP (0.5 mg/l). It was noted that the survival rate of explants is determined by the physiological state of the source material due to the corresponding agro-climatic conditions during the introduction period, as well as the genotypic characteristics of the varieties. Thus, explants isolated during the active growing season are characterized by a higher and more stable survival rate. When explants were cultivated in spring and autumn, the physiological state of the explants and their survival rate were influenced by the genotypic response of varieties to the corresponding agroclimatic conditions.

Modified elements of the technology of in vitro propagation of horticultural crops

2020 ◽  
Vol 61 ◽  
pp. 44-53
Author(s):  
O. V. Matushkina ◽  
I. N. Pronina ◽  
S. A. Matushkin ◽  
L. V. Kruzhkova
Keyword(s):  
In Vitro Propagation ◽  
Root Formation ◽  
Water Soluble ◽  
Advanced Technology ◽  
Ex Vitro ◽  
Labor Costs ◽  
Black Currant ◽  
Horticultural Crops ◽  
Clonal Micropropagation

The optimized technology for in vitro propagation of such horticultural crops as apple and pear clonal rootstocks, raspberry and black currant varieties is based on modification of culture factors and on realization of morphogenetic potential of the studied genotypes. It was found, that regeneration ability in vitro is determined not only by chemical factors of culturing, but as well as by genotypical peculiarities of plants. The paper regards the advanced technology peculiarities for clonal apple rootstock propagation where the application of water-soluble substances as mineral base allows to simplify the process of preparing the nutrient media. The number of micro-shoots with their length of more than 1.5 cm will be increased by 13.4-43.6 %, the root formation process will be accelerated by 1-2 weeks. Micro-shoot and the root system quality will be improved. The rooting ability occurred to be accelerated by 20.0-93.3 % and adaptability of ex vitro plants increased by 4.7-33.3 % and the yield of the regenerated plants over 6 passages by 1.1-27.0. All these measures allowed us to reduce labor costs by 16.7 % and Mo cost price by 52.01 roubles. The optimized technique for clonal micropropagation of promising raspberry varieties is based on rooting of microshoots directly in soil substrate, excluding the in vitro stage of rooting. The elimination of the rhizogenesis stage from in vitro cycle allows reducing the number of subcultures by one passage. The technique above allows us to reduce labor costs for production of Mo by 27.9 % and the cost of adapted ex vitro plant by 44.6 roubles. The improved technique for clonal micropropagation of promising black currant varieties allows us to better the micro-shoot quality and to increase the number of micro-shoots suitable for rooting by 6.2- 86,5 % depending ok genotype and use hormon free nutrient medium for root formation.

USE OF BIOTECHNOLOGICAL TECHNIQUES IN BLACK CURRANT REPRODUCTION

1970 ◽  
pp. 69-73
Author(s):  
Т. М. Khromova ◽  
L. V. Tashmatova ◽  
О. V. Matsneva ◽  
V. V. Shakhov
Keyword(s):  
Nutrient Medium ◽  
Culture Media ◽  
Biologically Active ◽  
Cultivated Plants ◽  
Quantitative Composition ◽  
Black Currant ◽  
Clonal Micropropagation ◽  
Emerging Issues ◽  
Selection Of

Currently, methods for the culture of isolated tissues and organs have been widely used to solve theoretical and applied problems of biotechnology. The article discusses the theoretical aspects of the use of biotechnological techniques in the reproduction of black currants (Ribes nigrum L.). The need for the use of biotechnological methods in the rapid reproduction of various forms and varieties of black currants due to the difficulty of reproduction of a number of genotypes by traditional methods, especially forms obtained using interspecific hybridization. Among the emerging issues are the following: find donors of valuable traits with the aim of increasing ecological plasticity of cultivated plants; a study of the influence of qualitative and quantitative composition of nutrient media for the cultivated plants, optimization of the timing of the introduction of plants and selection of optimal modifications of culture media with the varietal characteristics at different stages of cultivation; the study of the influence of hormonal and other biologically active substances on development of plants in conditions of culture in vitro. In the article literary sources, which describe those additional issues relating to process optimization clonal micropropagation of black currant. All stages of cultivation of the given culture in conditions in vitro from initiation to adaptation in non-sterile conditions are considered. The factors that determine the success of reproduction are described: terms of introduction into the culture and types of explants, selection of sterilizing agent, optimization of the nutrient medium depending on the passage, the influence of different components of the medium on regenerative plants. The methods of microclonal reproduction, developed by scientists of leading research organizations, data on ways to combat phenolic oxidation of the nutrient medium.

scholarly journals Effect of aqueous extracts of aspen, black currant, folded blackberry and walnut leaves on development of pathogenic fungi

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 623-625
Author(s):  
E. Stompor-Chrzan
Keyword(s):  
Juglans Regia ◽  
Fusarium Culmorum ◽  
Aqueous Extracts ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Inhibiting Effect ◽  
Walnut Leaves

The antifungal activity of water extracts of dried leaves of folded blackberry (Rubus plicatus W. et N.), walnut (Juglans regia L.), black currant (Ribes nigrum L.), and aspen (Populus tremula L.) was assessed in the in vitro experiment. The tested fungi were Alternaria alternata, Botrytis cinerea, Rhizoctonia solani, Fusarium culmorum, F. oxysporum and F. solani. It was found that all tested extracts inhibited the development of A. alternaria and F. oxysporum, whereas the strongest inhibiting effect was exhibited by extracts of aspen (A. alternata and F. oxysporum), black current (A. alternata) and folded blackberry (F. oxysporum). The tested extracts had no effect on B. cinerea.

scholarly journals Clonal propagation of black currant (Ribes nigrum L.)

2020 ◽  
Vol 19 (2) ◽  
pp. 005-010
Author(s):  
K. Yu. Guseva
Keyword(s):  
Gibberellic Acid ◽  
Growth Regulators ◽  
Clonal Propagation ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Rooting Medium

At the stage of transfer to soil, a scheme is defined that provides production of improved plantlets of thestudied varieties. 'Kuharsky's Memory' and 'Kanakhama' black сurrant varieties are characterized by high regenerativecapacity in vitro (the proportion of viable explants is 93.5 % and 96.4 %, respectively). At the multiplication stage, the influence of growth regulators (6-benzylaminopurine, gibberellic acid) on the morphogenesis of currant plantlets was studied. It shows their impact on such morphological indicators of development of plantlets as the height (cm), quantity, theaverage length of roots (cm). At the rooting stage, the concentration (0.5 mg/l) of auxin (β-indolylbutyric acid) was selected, the introduction of which into the rooting medium provided the maximum indicators of rhizogenesis: the numberand length of roots.

Fungus Spaeropsis Malorum peck. One of the currant stalk drying agents

2020 ◽  
Vol 62 ◽  
pp. 172-178
Author(s):  
S. E. Golovin
Keyword(s):  
Causative Agent ◽  
High Frequency ◽  
Frequency Of Occurrence ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Causative Agents ◽  
Pear Trees ◽  
Red Currant

The article highlights the results of research carried out in 2019-2020 to study the reasons for the drying out of the stems of black currant (Ribes nigrum L.), red currant (Ribes rubrum L.) and golden currant (Ribes aureum L.). As a result of research, the fungus Sphaeropsis malorum Peck. in the first in Russia it was revealed from the drying stems of three types of black currants, red currants and golden currants. This  pathogen inhabited currant stems together with fungi from the genus Cytospora spp., causative agents of stem drying. On red currants, it was established that S. malorum was the main causative agent of branch drying, as indicated by the high frequency of occurrence (75.0-83.3 %) of this fungus on the affected stems, compared with species from the genus Cytospora spp. (16.7-33.3 %) and Phomopsis ribesia (25.0 %). Due to the fact that the cultivation of currants in the immediate vicinity of apple and pear trees leads to infection of plants with the fungus S. malorum, it is necessary to avoid planting currants in the vicinity of fruit-bearing seed gardens.

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