scholarly journals Microevolutionary relationships between biotypes of Elymus confusus, E. peschkovae, and E. sibiricus (Poaceae) according to hybridization and sequencing of the nuclear gene GBSS1 (waxy)

2021 ◽  
Vol 38 ◽  
pp. 00002
Author(s):  
Alexander V. Agafonov ◽  
Maria V. Emtseva ◽  
Elena V. Shabanova (Kobozeva)

According to descriptions, the Siberian species E. peschkovae and E. confusus differ in the presence or absence of trichomes on lemma and rachilla surfaces only. Two methods were used for study microevolutionary relationships between these species: hybridization of biotypes from different locations and analysis of nucleotide sequences of the low-copy nuclear gene GBSS1. Created and grown hybrids in combinations AMU-8804 × BER-0807 and AMU-8804 × BUK-1109 showed complete seed sterility in two field vegetations. Taking into account wide species areas, 3 hybrids were created between Magadan biotypes from a common habitat in the combination E. confusus MOL1887 × E. peschkovae MOL-1882. The plants showed seed fertility (SF) of 0–3 %. This result does not obscure the possibility to assess SF in the F2 generation and the character of inheritance of diagnostic traits. A comparative study of the GBSS1 gene sequences in accessions was carried out in comparison with clones of reference species. Differentiation of clones by the St2 subgenome in E. confusus, E. peschkovae, E. sibiricus and E. caninus did not reveal a clear relationships between the species. Meanwhile, a certain species specificity for the H1 subgenome was noted, confirming the existence of microevolutionary isolation of these species.

2020 ◽  
Vol 24 ◽  
pp. 00001
Author(s):  
Alexander Agafonov ◽  
Maria Emtseva ◽  
Elena Shabanova (Kobozeva) ◽  
Sergey Asbaganov

A comparative study was made of the sequence of the GBSS1 gene fragment in accessions of closely related species Elymus margaritae, E. komarovii, and E. transbaicalensis from Siberia. Nucleotide sequences of the gene were determined for St subgenomes in accessions of E. margaritae and E. komarovii from classical habitats. The StH-genomic constitution was confirmed, and microevolutionary relationships between species were evaluated by constructing the NJ dendrogram. It was shown that variants of the St subgenomes in accessions E. margaritae GUK-1009 and E. komarovii AUK-9803 belong to the North American ancestral line St2 of the genus Pseudoroegneria, in contrast to accessions E. margaritae AUK-0650 and E. komarovii GAR-0501. The latter belong to the Asian branch of St1, together with variants of subgenomes in the species E. gmelinii and E. pendulinus. That is, according to the differentiation levels of the St subgenome, accession E. margaritae GUK-1009 (holotype) is significantly distanted from the accession AUK-0650 (paratype), which in turn is close to accessions of E. komarovii and E. transbaicalensis from East Sayan. According to levels of differentiation of the H subgenome, the studied species did not show noticeable differences.


2007 ◽  
Vol 111 (9) ◽  
pp. 1019-1029 ◽  
Author(s):  
Sigisfredo Garnica ◽  
Michael Weiss ◽  
Grit Walther ◽  
Franz Oberwinkler
Keyword(s):  

1992 ◽  
Vol 41 (4) ◽  
pp. 483 ◽  
Author(s):  
Timothy P. Friedlander ◽  
Jerome C. Regier ◽  
Charles Mitter

Zootaxa ◽  
2021 ◽  
Vol 4974 (2) ◽  
pp. 333-360
Author(s):  
KOJI TOJO ◽  
KEN MIYAIRI ◽  
YUTO KATO ◽  
AYANA SAKANO ◽  
TOMOYA SUZUKI

A new mayfly species, Bleptus michinokuensis sp. nov. (Ephemeroptera: Heptageniidae) is described on the basis of specimens of male and female adults and mature nymphs collected at a seepage zone of a small freshwater branch of the ‘Tachiya-zawa-gawa’ River located amongst the northern foothills of Mt. Gassan (Shonai-machi Town, Yamagata Prefecture, Japan). This new Bleptus species is characterized by its clear fore and hind wings. That is, they neither exhibit the distinct black band on the fore wings, nor the characteristic darkened margins along the edges of both the fore and hind wings. Rather it has a blackish colored terminal half of its fore legs (i.e., tibial, tarsal and pretarsal segments). These features differ clearly when comparing them to the other known species, Bleptus fasciatus Eaton. The information and data describing the habitat and distribution range of this new species are also noted. We also examined and discussed the genetic relationship of two Bleptus mayflies to settle the taxonomic status, inferred from the partially sequenced cytochrome c oxidase subunit I (COI) and large mitochondrial ribosomal subunit (16S rRNA) genes, and also the nuclear internal transcribed spacer 2 (ITS2) gene sequences. Consequently, phenetic and molecular phylogenetic analyses agreed well in terms of clustering. 


Zootaxa ◽  
2018 ◽  
Vol 4450 (3) ◽  
pp. 359 ◽  
Author(s):  
SIMON T. MADDOCK ◽  
MARK WILKINSON ◽  
DAVID J. GOWER

A new species of indotyphlid caecilian amphibian, Hypogeophis montanus sp. nov., is described based on a series of specimens from the Seychelles island of Mahé, collected from two localities in 2013 and 2015. The new species most closely resembles the Seychelles (Mahé) endemic H. brevis in being short (maximum known total length in life ca. 110 mm) and long snouted, but differs by having more vertebrae, a relatively smaller head, and substantially distinct mitochondrial and nuclear gene sequences. Hypogeophis montanus sp. nov. is known from higher elevations (718–731 m) than H. brevis (ca. 350–650 m), and its elevationally restricted distribution on a single small island likely renders it threatened under IUCN Red List criteria. Hypogeophis montanus sp. nov. is the third species of small and long-snouted caecilian reported from the Seychelles. Along with H. brevis and H. pti, H. montanus sp. nov. is among the smallest known species of caecilian and possibly has the smallest global distribution. 


Taxon ◽  
2020 ◽  
Vol 69 (1) ◽  
pp. 67-86
Author(s):  
Carole Sinou ◽  
Warren Cardinal‐McTeague ◽  
Anne Bruneau
Keyword(s):  

1986 ◽  
Vol 6 (2) ◽  
pp. 645-652 ◽  
Author(s):  
J Geliebter ◽  
R A Zeff ◽  
D H Schulze ◽  
L R Pease ◽  
E H Weiss ◽  
...  

Genetic interaction as a mechanism for the generation of mutations is suggested by recurrent, multiple nucleotide substitutions that are identical to nucleotide sequences elsewhere in the genome. We have sequenced the mutant K gene from the bm6 mouse, which is one of a series of eight closely related, yet independently occurring mutants known collectively as the "bg series." Two changes from the Kb gene are found, positioned 15 nucleotides apart: an A-to-T change and a T-to-C change in the codons corresponding to amino acids 116 and 121, resulting in Tyr-to-Phe and Cys-to-Arg substitutions, respectively. Hybridization analysis with an oligonucleotide specific for the altered Kbm6 sequence identifies one donor gene, Q4, located in the Qa region of the H-2 complex. The two altered nucleotides that differentiate Kbm6 and Kb are present in Q4 in a region where Kb and Q4 are otherwise identical for 95 nucleotides, delineating the maximum genetic transfer between the two genes. Because the Kbm6 mutation arose in an homozygous mouse these data indicate that the Q4 gene contains the only donor sequence and demonstrates that Q-region gene sequences can interact with the Kb gene to generate variant K molecules.


2020 ◽  
Vol 65 (3) ◽  
pp. 493-502 ◽  
Author(s):  
Satoko Mori ◽  
Thidalay Thwe ◽  
Wai Min Thu ◽  
Shumpei P. Yasuda ◽  
Saw Bawm ◽  
...  

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