scholarly journals Use of 2-aminoethanesulfonic acid (taurine) for cryopreservation and storage of Siberian sturgeon sperm

2021 ◽  
Vol 273 ◽  
pp. 03010
Author(s):  
Konstantin Kovalev ◽  
Olga Dokina ◽  
Natalia Pronina ◽  
Dmitry Balashov ◽  
Aleksandra Krasilnikova
Keyword(s):  
Fertility Preservation ◽  
Siberian Sturgeon ◽  
Taurine Concentration ◽  
And Storage ◽  
Long Term Preservation

The research aimed to determine the effect of different taurine concentrations on the duration of sperm fertility preservation and the results of further use during cryopreservation. The taurine was injected into the sperm of the Siberian sturgeon by various methods under conditions of low positive temperatures. Immediately after preparation, the taurine was injected into native sperm at concentrations of 0.01, 0.05, and 0.1 mmol/ml. Two methods of introducing taurine into native sperm were tested: 1 (dry method) - adding taurine powder directly to native sperm and 2 (wet method) - adding taurine dissolved in saline to native sperm. The studies have shown that injections of taurine in an amount of 0.05 - 0.1 mmol/ml both dry and wet can be recommended as the method of long-term preservation of the native sperm of Siberian sturgeon viability. While storing Siberian sturgeon sperm for further cryopreservation the optimal taurine concentration is 0.01 mmol/ml.

scholarly journals Preservation of Azotobacter chroococcum vegetative cells in dry polymers

2014 ◽  
Vol 20 (2) ◽  
pp. 201 ◽  
Author(s):  
Daniel Rojas-Tapias ◽  
Oriana Ortega Sierra ◽  
Diego Rivera Botía ◽  
Ruth Bonilla
Keyword(s):  
Storage Conditions ◽  
Azotobacter Chroococcum ◽  
Bacterial Degradation ◽  
Protective Agent ◽  
Degradation Rates ◽  
Different Temperatures ◽  
And Storage ◽  
Temperature Time ◽  
Long Term Preservation

We studied the preservation of Azotobacter chroococcum C26 using three dry polymers: carrageenin, sodium alginate, and HPMC, using a method of accelerated degradation. Bacterial viability, as response variable, was measured at three temperatures in four different times, which was followed by calculation of bacterial degradation rates. Results showed that temperature, time of storage, and protective agent influenced both viability and degradation rates (P;lt;0.05). We observed, using the Arrhenius thermodynamic model, that the use of polymers increased the activation energy of bacterial degradation compared to control. We obtained thermodynamic models for each polymer, based on the Arrhenius equation, which predicted the required time for thermal degradation of the cells at different temperatures. Analysis of the models showed that carrageenin was the best polymer to preserve A. chroococcum C26 since ~ 900 days are required at 4 ºC to reduce its viability in two log units. We conclude, therefore, that long-term preservation of A. chroococcum C26 using dry polymers is suitable under adequate preservation and storage conditions.

scholarly journals Long-Term Preservation and Storage of Mycobacteria

1972 ◽  
Vol 24 (3) ◽  
pp. 311-317 ◽  
Author(s):  
Thomas H. Kim ◽  
George P. Kubica
Keyword(s):  
And Storage ◽  
Long Term Preservation

scholarly journals Long-Term Preservation and Storage of Mycobacteria

1972 ◽  
Vol 24 (3) ◽  
pp. 311-317 ◽  
Author(s):  
Thomas H. Kim ◽  
George P. Kubica
Keyword(s):  
And Storage ◽  
Long Term Preservation

scholarly journals Do not Dispose of Historic Fluid Collections: Evaluating Research Potential and Range of Use

2020 ◽  
Vol 34 (1) ◽  
pp. 157-169
Author(s):  
M. Herbin ◽  
M.D. Santin ◽  
R. Toro ◽  
K. Heuer ◽  
J. Cuisin ◽  
...  
Keyword(s):  
Analytical Methods ◽  
Future Research ◽  
Museum Professionals ◽  
Determining Factors ◽  
Formaldehyde Exposure ◽  
Fluid Collections ◽  
And Storage ◽  
Long Term Preservation ◽  
Over Time

Abstract The use of specific preservative solutions by museum professionals to maintain fluid-preserved specimens has fluctuated over the years with advances in chemistry. The determining factors for the original choice of solution closely correlate with the historical parameters and original usage of the collections. Consequently, for any given collection, changes and substitutions over time in the types of preservative fluids used have likely occurred. The present comparative analysis of the state of brain preservation, carried out at macroscopic, microscopic, and molecular levels, allowed us to evaluate the effect of the different treatments applied over time to fluid-preserved collections. Our results confirm that the duration of formaldehyde exposure of the tissues clearly has an effect on their long-term preservation. Despite the controversies associated with the quality or use of some historic fixatives, modern analytical methods such as medical imagery reveal the preservation quality in historic specimens and their potential for future research use. However, the choice of fixatives and storage fluids to preserve the specimens is of critical importance because today's choices will influence the use of the specimen for advanced analytical methods in the future.

Problems of treatment of primary openangle glaucoma in combination with thrombosis of the central retinal vein

GlaucomaNews ◽  
2020 ◽  
pp. 65-69
Author(s):  
T.E. Lipatkina ◽  
◽  
Е.V. Karlova ◽  
A.V. Zolotarev ◽  
◽  
...  
Keyword(s):  
Macular Edema ◽  
Primary Open Angle Glaucoma ◽  
Antihypertensive Drugs ◽  
Open Angle Glaucoma ◽  
Vascular Wall ◽  
Open Angle ◽  
Retinal Edema ◽  
Long Term Preservation ◽  
Central Retinal Vein

Patients with primary open-angle glaucoma (POAG) and ophthalmic hypertension have an increased likelihood of developing occlusions (thrombosis) of the central retinal vein. Different groups of antihypertensive drugs differ in their mechanism of action and may affect concomitant ocular pathology, in particular, retinal edema, which occurs, for example, in occlusion of the central retinal vein. Used in most patients with glaucoma, prostaglandin analogs can contribute to the long-term preservation of macular edema due to the effect on the permeability of the vascular wall. Preparations of other pharmacological groups, reducing the production of aqueous humor, on the contrary, may contribute to its regression. Therefore, the question of choosing a drug for antihypertensive therapy in patients with primary open-angle glaucoma and concomitant macular edema is relevant and is for further study.

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