scholarly journals Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) HMGS Gene

2021 ◽  
Vol 292 ◽  
pp. 03094
Author(s):  
Meiwei Zhao ◽  
Tao Zhang ◽  
Lei Yang ◽  
Hongtao Feng ◽  
Zhengxiong Zhao
Keyword(s):  
Nicotiana Tabacum ◽  
Withania Somnifera ◽  
Profile Analysis ◽  
Nicotiana Sylvestris ◽  
Tissue Expression ◽  
Nicotiana Attenuata ◽  
Reading Frame ◽  
Expression Profile Analysis ◽  
Tissue Expression Profile ◽  
Rapid Amplification

3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGS) is a member of condensing enzymes that catalyze a Claisen-like condensation reaction.The tobacco (nicotiana tabacum) HMGS gene was firstly characterized using the rapid amplification of cDNA ends methods based on one tobacco EST. The full-length tobacco HMGS gene mRNA was 1,773bp containing a 1389 bp open reading frame, which encodes a protein of 462 amino acids. Sequence analysis revealed that the HMGS of tobacco shares high homology with the HMGS of nicotiana tomentosiformis (96%), nicotiana attenuata (95%), Nicotiana sylvestris (95%), nicotiana benthamiana(94%), solanum lycopersicum(94%), solanum tuberosum(93%) and withania somnifera(93%). Results also showed that tobacco HMGS gene has a closer genetic relationship with the HMGS gene of withania somnifera. Tissue expression profile analysis revealed that the tobacco HMGS gene was highly expressed in flower, but moderately expressed in leaf and stem, and weakly expressed in root. Our experiment established the foundation for further research on this tobacco gene.

scholarly journals Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) GMP Synthase Gene

2021 ◽  
Vol 292 ◽  
pp. 03070
Author(s):  
Meiwei Zhao ◽  
Lei Yang ◽  
Jiacan Wu ◽  
Haijuan Wang ◽  
Zhengxiong Zhao
Keyword(s):  
Nicotiana Tabacum ◽  
Profile Analysis ◽  
Tissue Expression ◽  
Guanosine Monophosphate ◽  
Nicotiana Attenuata ◽  
Reading Frame ◽  
Lycopersicon Pennellii ◽  
Expression Profile Analysis ◽  
Tissue Expression Profile ◽  
Rapid Amplification

The complete mRNA sequence of one tobacco (nicotiana tabacum) gene—guanosine monophosphate (GMP)synthase, was amplified using the rapid amplification of cDNA ends methods. The full-length tobacco GMP synthase gene mRNA was 2,127bp containing a 1,617 bp open reading frame, which encodes a protein of 538 amino acids. Sequence analysis revealed that the GMP synthase of tobacco shares high homology with the GMP synthase of wood tobacco(99%), nicotiana attenuata(99%), nicotiana tomentosiformis(99%), potato(92%), Lycopersicon pennellii(92%), lycopersicon esculentum(92%), capsicum annuum(91%), capsicum chinense(91%) and capsicum baccatum(90%). BLAST analysis within the tobacco high throughout genomic sequences database revealed that this gene has 5 introns and 6 exons. Results also showed that tobacco GMP synthase gene has a closer genetic relationship with the GMP synthase gene of wood tobacco. Tissue expression profile analysis revealed that the tobacco GMP synthase gene was highly expressed in leaf, but moderately expressed in root, flower and stem. Our experiment established the foundation for further research on this tobacco gene.

scholarly journals Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) 2-Hydroxyisoflavanone Dehydratasedase Gene

2021 ◽  
Vol 292 ◽  
pp. 03098
Author(s):  
Meiwei Zhao ◽  
Song Miao ◽  
Jun Guo ◽  
Yongyu Li ◽  
Zhengxiong Zhao
Keyword(s):  
Nicotiana Tabacum ◽  
Profile Analysis ◽  
Tissue Expression ◽  
Reading Frame ◽  
Lycopersicon Pennellii ◽  
Nicotiana Tomentosiformis ◽  
Expression Profile Analysis ◽  
Tissue Expression Profile ◽  
Rapid Amplification ◽  
Single Exon Gene

The complete mRNA sequence of one tobacco (nicotiana tabacum) gene—2-hydroxyisoflavanone dehydratasedase, was amplified using the rapid amplification of cDNA ends methods based on one tobacco EST. The full-length tobacco 2-hydroxyisoflavanone dehydratasedase gene mRNA was 1,278bp containing a 966 bp open reading frame, which encodes a protein of 321 amino acids. Sequence analysis revealed that the 2-hydroxyisoflavanone dehydratasedase of tobacco shares high homology with the 2-hydroxyisoflavanone dehydratasedase of nicotiana tomentosiformis(99%), capsicum annuum(78%), potato(75%), lycopersicon pennellii(73%) and lycopersicon esculentum(72%). BLAST analysis within the tobacco high throughout genomic sequences database revealed that this gene has no intron and is a single exon gene. Results also showed that tobacco 2-hydroxyisoflavanone dehydratasedase gene has a closer genetic relationship with the 2-hydroxyisoflavanone dehydratasedase gene of nicotiana tomentosiformis. Tissue expression profile analysis revealed that the tobacco 2-hydroxyisoflavanone dehydratasedase gene was highly expressed in leaf and flower, but moderately expressed in root and stem. Our experiment established the foundation for further research on this tobacco gene.

scholarly journals Genome-Wide Identification of Barley ABC Genes and Their Expression in Response to Abiotic Stress Treatment

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1281
Author(s):  
Ziling Zhang ◽  
Tao Tong ◽  
Yunxia Fang ◽  
Junjun Zheng ◽  
Xian Zhang ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Amino Acid ◽  
Stress Responses ◽  
Transmembrane Domain ◽  
Profile Analysis ◽  
Tissue Expression ◽  
Expression Profile Analysis ◽  
Genome Wide ◽  
Tissue Expression Profile ◽  
Amino Acid Length

Adenosine triphosphate-binding cassette transporters (ABC transporters) participate in various plant growth and abiotic stress responses. In the present study, 131 ABC genes in barley were systematically identified using bioinformatics. Based on the classification method of the family in rice, these members were classified into eight subfamilies (ABCA–ABCG, ABCI). The conserved domain, amino acid composition, physicochemical properties, chromosome distribution, and tissue expression of these genes were predicted and analyzed. The results showed that the characteristic motifs of the barley ABC genes were highly conserved and there were great diversities in the homology of the transmembrane domain, the number of exons, amino acid length, and the molecular weight, whereas the span of the isoelectric point was small. Tissue expression profile analysis suggested that ABC genes possess non-tissue specificity. Ultimately, 15 differentially expressed genes exhibited diverse expression responses to stress treatments including drought, cadmium, and salt stress, indicating that the ABCB and ABCG subfamilies function in the response to abiotic stress in barley.

scholarly journals Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) Sucrose Synthase Gene

2021 ◽  
Vol 292 ◽  
pp. 03101
Author(s):  
Meiwei Zhao ◽  
Juan Zou ◽  
Sixuan Leng ◽  
Ying Tao ◽  
Zhengxiong Zhao
Keyword(s):  
Nicotiana Tabacum ◽  
Sucrose Synthase ◽  
Dna Analysis ◽  
Pattern Analysis ◽  
Profile Analysis ◽  
Expression Pattern Analysis ◽  
Expression Profile Analysis ◽  
Initial Characterization ◽  
Rapid Amplification ◽  
Gene Mrna

A novel tobacco(nicotiana tabacum) sucrose synthase genewas characterized using the rapid amplification of cDNA ends methods based on one tobacco EST. Thissucrose synthase gene mRNA was 2,954bp in length containing a 2,418bp open reading framewhich encodes a protein of 805 amino acids. Sequence analysis revealed that thissucrose synthase of tobacco shares high homology with the sucrose synthase of Nicotianaattenuata(98%), Nicotianasylvestris(98%), Nicotianatomentosiformis(98%), Capsicumannuum(95%), Capsicumbaccatum(95%), Solanumtuberosum(94%), Solanumlycopersicum(94%) and Solanumpennellii(94%). Results also showed that tobacco sucrose synthase gene has a closer genetic relationship with the sucrose synthase gene of Capsicumannuum.Genomic DNA analysis indicated that tobacco sucrose synthase gene is structured in 13 exons and 12 introns.Tissue expression profile analysis revealed that this tobacco sucrose synthase gene was highly expressed in leaf, but moderately expressed in root, stemand flower. These established the foundation for further research on the tobacco sucrose synthase gene.

scholarly journals Identification and Characterization of Secondary Wall-Associated NAC Genes and Their Involvement in Hormonal Responses in Tobacco (Nicotiana tabacum)

2021 ◽  
Vol 12 ◽  
Author(s):  
Na Xu ◽  
Lin Meng ◽  
Lin Song ◽  
Xiaoxu Li ◽  
Shasha Du ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Stress Responses ◽  
Secondary Wall ◽  
Profile Analysis ◽  
Nicotiana Sylvestris ◽  
Wall Thickening ◽  
Nac Domain ◽  
Qrt Pcr ◽  
Transactivation Assay ◽  
Expression Profile Analysis

Secondary wall-associated NAC (SWN) genes are a subgroup of NAC (NAM, ATAF, and CUC) transcription factors (TF) that play a key role in regulating secondary cell wall biosynthesis in plants. However, this gene family has not been systematically characterized, and their potential roles in response to hormones are unknown in Nicotiana tabacum. In this study, a total of 40 SWN genes, of which 12 from Nicotiana tomentosiformis, 13 from Nicotiana sylvestris, and 15 from Nicotiana tabacum, were successfully identified. The 15 SWNs from Nicotiana tabacum were further classified into three groups, namely, vascular-related NAC domain genes (NtVNDs), NAC secondary wall thickening promoting factor genes (NtNSTs), and secondary wall-associated NAC domain genes (NtSNDs). The protein characteristic, gene structure, and chromosomal location of 15 NtSWNs (also named Nt1 to Nt15) were also analyzed. The NtVND and NtNST group genes had five conserved subdomains in their N-terminal regions and a motif (LP[Q/x] L[E/x] S[P/A]) in their diverged C- terminal regions. Some hormones, dark and low-temperature related cis-acting elements, were significantly enriched in the promoters of NtSWN genes. A comprehensive expression profile analysis revealed that Nt4 and Nt12 might play a role in vein development. Others might be important for stem development. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) revealed that in the NtNST group, genes such as Nt7, Nt8, and Nt13 were more sensitive than the genes in NtVND and NtSND groups under abiotic stress conditions. A transactivation assay further suggested that Nt7, Nt8, and Nt13 showed a significant transactivation activity. Overall, SWN genes were finally identified and characterized in diploid and tetraploid tobacco, revealing new insights into their evolution, variation, and homology relationships. Transcriptome, cis-acting element, qRT-PCR, and transactivation assay analysis indicated the roles in hormonal and stress responses, which provided further resources in molecular mechanism and genetic improvement.

scholarly journals A novel porcine gene-<i>MGLL</i>, differentially expressed in the backfat tissues from Meishan and Large White pigs

2010 ◽  
Vol 53 (5) ◽  
pp. 555-563
Author(s):  
Y. Dawei ◽  
B. Baoliang ◽  
L. Yonggang
Keyword(s):  
Tissue Expression ◽  
Complete Sequence ◽  
Differentially Expressed ◽  
Computer Assisted ◽  
Large White ◽  
Reading Frame ◽  
Tissue Expression Analysis ◽  
Red Jungle Fowl ◽  
Monoglyceride Lipase ◽  
Rapid Amplification

Abstract. One novel gene that was differentially expressed was identified through semi-quantitative RT-PCR and the cDNA complete sequence was then obtained using the rapid amplification of cDNA ends (RACE) method. Nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 303 amino acids has high homology with the monoglyceride lipase (MGLL) of eight species-dog (91 %), human (89 %), cattle (88 %), platypus (83 %), mouse (82 %), rat (81 %), rhesus monkey (80 %), and red jungle fowl (70 %) – so that it can be defined as swine MGLL gene. This gene is structured in seven exons and six introns as revealed by computer-assisted analysis. The tissue expression analysis indicated that the swine MGLL gene is differentially expressed in detected tissues. Our experiment suggested that the swine MGLL gene might play an important roles in the superabundant fat deposition of Chinese pigs.

scholarly journals Pax3 Gene Regulated Melanin Synthesis by Tyrosinase Pathway in Pteria penguin

2018 ◽  
Vol 19 (12) ◽  
pp. 3700 ◽  
Author(s):  
Feifei Yu ◽  
Bingliang Qu ◽  
Dandan Lin ◽  
Yuewen Deng ◽  
Ronglian Huang ◽  
...  
Keyword(s):  
Total Content ◽  
Tissue Expression ◽  
Melanin Synthesis ◽  
Mizuhopecten Yessoensis ◽  
Pax3 Gene ◽  
Pteria Penguin ◽  
Tissue Expression Profile ◽  
Polymerase Chain ◽  
Rapid Amplification ◽  
Race Pcr

The paired-box 3 (Pax3) is a transcription factor and it plays an important part in melanin synthesis. In this study, a new Pax3 gene was identified from Pteria penguin (Röding, 1798) (P. penguin) by RACE-PCR (rapid-amplification of cDNA ends-polymerase chain reaction) and its effect on melanin synthesis was deliberated by RNA interference (RNAi). The cDNA of PpPax3 was 2250 bp long, containing an open reading fragment of 1365 bp encoding 455 amino acids. Amino acid alignment and phylogenetic tree showed PpPax3 shared the highest (69.2%) identity with Pax3 of Mizuhopecten yessoensis. Tissue expression profile showed that PpPax3 had the highest expression in mantle, a nacre-formation related tissue. The PpPax3 silencing significantly inhibited the expression of PpPax3, PpMitf, PpTyr and PpCdk2, genes involved in Tyr-mediated melanin synthesis, but had no effect on PpCreb2 and an increase effect on PpBcl2. Furthermore, the PpPax3 knockdown obviously decreased the tyrosinase activity, the total content of eumelanin and the proportion of PDCA (pyrrole-2,3-dicarboxylic acid) in eumelanin, consistent with influence of tyrosinase (Tyr) knockdown. These data indicated that PpPax3 played an important regulating role in melanin synthesis by Tyr pathway in P. penguin.

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