scholarly journals Pax3 Gene Regulated Melanin Synthesis by Tyrosinase Pathway in Pteria penguin

2018 ◽  
Vol 19 (12) ◽  
pp. 3700 ◽  
Author(s):  
Feifei Yu ◽  
Bingliang Qu ◽  
Dandan Lin ◽  
Yuewen Deng ◽  
Ronglian Huang ◽  
...  
Keyword(s):  
Total Content ◽  
Tissue Expression ◽  
Melanin Synthesis ◽  
Mizuhopecten Yessoensis ◽  
Pax3 Gene ◽  
Pteria Penguin ◽  
Tissue Expression Profile ◽  
Polymerase Chain ◽  
Rapid Amplification ◽  
Race Pcr

The paired-box 3 (Pax3) is a transcription factor and it plays an important part in melanin synthesis. In this study, a new Pax3 gene was identified from Pteria penguin (Röding, 1798) (P. penguin) by RACE-PCR (rapid-amplification of cDNA ends-polymerase chain reaction) and its effect on melanin synthesis was deliberated by RNA interference (RNAi). The cDNA of PpPax3 was 2250 bp long, containing an open reading fragment of 1365 bp encoding 455 amino acids. Amino acid alignment and phylogenetic tree showed PpPax3 shared the highest (69.2%) identity with Pax3 of Mizuhopecten yessoensis. Tissue expression profile showed that PpPax3 had the highest expression in mantle, a nacre-formation related tissue. The PpPax3 silencing significantly inhibited the expression of PpPax3, PpMitf, PpTyr and PpCdk2, genes involved in Tyr-mediated melanin synthesis, but had no effect on PpCreb2 and an increase effect on PpBcl2. Furthermore, the PpPax3 knockdown obviously decreased the tyrosinase activity, the total content of eumelanin and the proportion of PDCA (pyrrole-2,3-dicarboxylic acid) in eumelanin, consistent with influence of tyrosinase (Tyr) knockdown. These data indicated that PpPax3 played an important regulating role in melanin synthesis by Tyr pathway in P. penguin.

scholarly journals Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) 2-Hydroxyisoflavanone Dehydratasedase Gene

2021 ◽  
Vol 292 ◽  
pp. 03098
Author(s):  
Meiwei Zhao ◽  
Song Miao ◽  
Jun Guo ◽  
Yongyu Li ◽  
Zhengxiong Zhao
Keyword(s):  
Nicotiana Tabacum ◽  
Profile Analysis ◽  
Tissue Expression ◽  
Reading Frame ◽  
Lycopersicon Pennellii ◽  
Nicotiana Tomentosiformis ◽  
Expression Profile Analysis ◽  
Tissue Expression Profile ◽  
Rapid Amplification ◽  
Single Exon Gene

The complete mRNA sequence of one tobacco (nicotiana tabacum) gene—2-hydroxyisoflavanone dehydratasedase, was amplified using the rapid amplification of cDNA ends methods based on one tobacco EST. The full-length tobacco 2-hydroxyisoflavanone dehydratasedase gene mRNA was 1,278bp containing a 966 bp open reading frame, which encodes a protein of 321 amino acids. Sequence analysis revealed that the 2-hydroxyisoflavanone dehydratasedase of tobacco shares high homology with the 2-hydroxyisoflavanone dehydratasedase of nicotiana tomentosiformis(99%), capsicum annuum(78%), potato(75%), lycopersicon pennellii(73%) and lycopersicon esculentum(72%). BLAST analysis within the tobacco high throughout genomic sequences database revealed that this gene has no intron and is a single exon gene. Results also showed that tobacco 2-hydroxyisoflavanone dehydratasedase gene has a closer genetic relationship with the 2-hydroxyisoflavanone dehydratasedase gene of nicotiana tomentosiformis. Tissue expression profile analysis revealed that the tobacco 2-hydroxyisoflavanone dehydratasedase gene was highly expressed in leaf and flower, but moderately expressed in root and stem. Our experiment established the foundation for further research on this tobacco gene.

scholarly journals Complete Genome Sequence of a Putative Novel Ilarvirus Isolated From Eleocharis Dulcis

2021 ◽  
Author(s):  
Lanqing Lv ◽  
Xinyang Wu ◽  
Jiajia Weng ◽  
Yuchao Lai ◽  
Kelei Han ◽  
...  
Keyword(s):  
Genomic Sequence ◽  
Phylogenetic Analyses ◽  
Genetic Distances ◽  
Open Reading Frames ◽  
Serological Reaction ◽  
Water Chestnut ◽  
Polymerase Chain ◽  
Rapid Amplification ◽  
Race Pcr ◽  
Next Generation Sequencing Ngs

Abstract The complete genomic sequence of a novel ilarvirus from Eleocharis dulcis, tentatively named water chestnut virus A (WCVA), was determined using next generation sequencing (NGS) combined with reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The three genomic RNA components of WCVA were 3578 (RNA1), 2873 (RNA2) and 2073 (RNA3) nucleotides long, with four predicted open reading frames containing conserved domains and motifs typical of ilarviruses. Phylogenetic analyses of each predicted protein consistently placed WCVA in subgroup 4 of the genus Ilarvirus, together with prune dwarf virus, viola white distortion associated virus, fragaria chiloensis latent virus and potato yellowing virus. The genetic distances and lack of serological reaction to antisera of other ilarviruses suggest that WCVA is a novel member of the genus.

scholarly journals Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) HMGS Gene

2021 ◽  
Vol 292 ◽  
pp. 03094
Author(s):  
Meiwei Zhao ◽  
Tao Zhang ◽  
Lei Yang ◽  
Hongtao Feng ◽  
Zhengxiong Zhao
Keyword(s):  
Nicotiana Tabacum ◽  
Withania Somnifera ◽  
Profile Analysis ◽  
Nicotiana Sylvestris ◽  
Tissue Expression ◽  
Nicotiana Attenuata ◽  
Reading Frame ◽  
Expression Profile Analysis ◽  
Tissue Expression Profile ◽  
Rapid Amplification

3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGS) is a member of condensing enzymes that catalyze a Claisen-like condensation reaction.The tobacco (nicotiana tabacum) HMGS gene was firstly characterized using the rapid amplification of cDNA ends methods based on one tobacco EST. The full-length tobacco HMGS gene mRNA was 1,773bp containing a 1389 bp open reading frame, which encodes a protein of 462 amino acids. Sequence analysis revealed that the HMGS of tobacco shares high homology with the HMGS of nicotiana tomentosiformis (96%), nicotiana attenuata (95%), Nicotiana sylvestris (95%), nicotiana benthamiana(94%), solanum lycopersicum(94%), solanum tuberosum(93%) and withania somnifera(93%). Results also showed that tobacco HMGS gene has a closer genetic relationship with the HMGS gene of withania somnifera. Tissue expression profile analysis revealed that the tobacco HMGS gene was highly expressed in flower, but moderately expressed in leaf and stem, and weakly expressed in root. Our experiment established the foundation for further research on this tobacco gene.

scholarly journals Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) GMP Synthase Gene

2021 ◽  
Vol 292 ◽  
pp. 03070
Author(s):  
Meiwei Zhao ◽  
Lei Yang ◽  
Jiacan Wu ◽  
Haijuan Wang ◽  
Zhengxiong Zhao
Keyword(s):  
Nicotiana Tabacum ◽  
Profile Analysis ◽  
Tissue Expression ◽  
Guanosine Monophosphate ◽  
Nicotiana Attenuata ◽  
Reading Frame ◽  
Lycopersicon Pennellii ◽  
Expression Profile Analysis ◽  
Tissue Expression Profile ◽  
Rapid Amplification

The complete mRNA sequence of one tobacco (nicotiana tabacum) gene—guanosine monophosphate (GMP)synthase, was amplified using the rapid amplification of cDNA ends methods. The full-length tobacco GMP synthase gene mRNA was 2,127bp containing a 1,617 bp open reading frame, which encodes a protein of 538 amino acids. Sequence analysis revealed that the GMP synthase of tobacco shares high homology with the GMP synthase of wood tobacco(99%), nicotiana attenuata(99%), nicotiana tomentosiformis(99%), potato(92%), Lycopersicon pennellii(92%), lycopersicon esculentum(92%), capsicum annuum(91%), capsicum chinense(91%) and capsicum baccatum(90%). BLAST analysis within the tobacco high throughout genomic sequences database revealed that this gene has 5 introns and 6 exons. Results also showed that tobacco GMP synthase gene has a closer genetic relationship with the GMP synthase gene of wood tobacco. Tissue expression profile analysis revealed that the tobacco GMP synthase gene was highly expressed in leaf, but moderately expressed in root, flower and stem. Our experiment established the foundation for further research on this tobacco gene.

scholarly journals Modulation of the Tissue Expression Pattern of Zebrafish CRP-Like Molecules Suggests a Relevant Antiviral Role in Fish Skin

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 78
Author(s):  
Melissa Bello-Perez ◽  
Mikolaj Adamek ◽  
Julio Coll ◽  
Antonio Figueras ◽  
Beatriz Novoa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bacterial Infections ◽  
Quantitative Polymerase Chain Reaction ◽  
Tissue Expression ◽  
Interferon Response ◽  
Fish Skin ◽  
Tissue Expression Pattern ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Antiviral Role

Recent studies suggest that short pentraxins in fish might serve as biomarkers for not only bacterial infections, as in higher vertebrates including humans, but also for viral ones. These fish orthologs of mammalian short pentraxins are currently attracting interest because of their newly discovered antiviral activity. In the present work, the modulation of the gene expression of all zebrafish short pentraxins (CRP-like proteins, CRP1-7) was extensively analyzed by quantitative polymerase chain reaction. Initially, the tissue distribution of crp1-7 transcripts and how the transcripts varied in response to a bath infection with the spring viremia of carp virus, were determined. The expression of crp1-7 was widely distributed and generally increased after infection (mostly at 5 days post infection), except for crp1 (downregulated). Interestingly, several crp transcription levels significantly increased in skin. Further assays in mutant zebrafish of recombinant activation gene 1 (rag1) showed that all crps (except for crp2, downregulated) were already constitutively highly expressed in skin from rag1 knockouts and only increased moderately after viral infection. Similar results were obtained for most mx isoforms (a reporter gene of the interferon response), suggesting a general overcompensation of the innate immunity in the absence of the adaptive one.

Cloning and functional expression of α-galactosidase cDNA from Penicillium janczewskii zaleski

Biologia ◽  
2011 ◽  
Vol 66 (2) ◽  
Author(s):  
Bo Zhang ◽  
Yiqun Chen ◽  
Zhimin Li ◽  
Wenqing Lu ◽  
Yunhe Cao
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Functional Expression ◽  
Recombinant Enzyme ◽  
Methanol Induction ◽  
Potential Applications ◽  
Prospective Application ◽  
Polymerase Chain ◽  
Penicillium Janczewskii ◽  
Rapid Amplification ◽  
Different Levels

AbstractIn recent years, α-galactosidase has been attracting more and more attention because of its potential applications in many aspects. Using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends, a full-length cDNA sequence composed of 2,439 bp was cloned from Penicillium janczewskii zaleski and was subcloned into pPICZαA and transformed into Pichia pastoris strain X-33. In a 10-L fermentor, the recombinant yeast expressed α-galactosidase with a yield of 254 U/mL by methanol induction for 120 h. The recombinant enzyme showed the optimal activity at 40°C and pH 5.2. The K m values of the recombinant enzyme using p-nitrophenyl-α-D-galactopyranoside (pNPG), melibiose, raffinose and stachyose as substrates were 1, 16, 17.8 and 5.3 mM, respectively. V max values were 227.3, 116.7, 104.8, and 80.6 μM/min using pNPG, melibiose, raffinose and stachyose as substrates, respectively. The α-galactosidase exhibited no sensitivity to various metal ions and ethylenediaminetetraacetic acid, and hydrolyzed melibiose, raffinose and stachyose with different levels of galactose release. The biochemical characteristics of the α-galactosidase suggest that the enzyme may have a prospective application in feed industry as an additive.

scholarly journals Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production

Viruses ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 802 ◽  
Author(s):  
Hadad ◽  
Luria ◽  
Smith ◽  
Sela ◽  
Lachman ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Virus Disease ◽  
Middle Eastern ◽  
Sequencing Analysis ◽  
Disease Symptoms ◽  
Next Generation Sequencing Analysis ◽  
Nutrition Deprivation ◽  
Rapid Amplification ◽  
Race Pcr ◽  
Complete Viral Genome

In a survey conducted in Cannabis sativa L. (cannabis) authorized farms in Israel, plants showed disease symptoms characteristic of nutrition deprivation. Interveinal chlorosis, brittleness, and occasional necrosis were observed in older leaves. Next generation sequencing analysis of RNA extracted from symptomatic leaves revealed the presence of lettuce chlorosis virus (LCV), a crinivirus that belongs to the Closteroviridae family. The complete viral genome sequence was obtained using RT-PCR and Rapid Amplification of cDNA Ends (RACE) PCR followed by Sanger sequencing. The two LCV RNA genome segments shared 85–99% nucleotide sequence identity with LCV isolates from GenBank database. The whitefly Bemisia tabaci Middle Eastern Asia Minor1 (MEAM1) biotype transmitted the disease from symptomatic cannabis plants to un-infected ‘healthy’ cannabis, Lactuca sativa, and Catharanthus roseus plants. Shoots from symptomatic cannabis plants, used for plant propagation, constituted a primary inoculum of the disease. To the best of our knowledge, this is the first report of cannabis plant disease caused by LCV.

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