scholarly journals Immunocytochemical detection of acrosomal damage following cold shock : loss of acrosin from the acrosomal region of ram, bull and boar spermatozoa

1980 ◽  
Vol 20 (6) ◽  
pp. 1801-1810 ◽  
Author(s):  
R. A. P. HARRISON ◽  
J. E. FLÉCHON
Keyword(s):  
Cold Shock ◽  
Immunocytochemical Detection ◽  
Boar Spermatozoa ◽  
Shock Loss

Effect of Incubation and Cold Shock on Motility of Boar Spermatozoa and Their Relationship to Lipid Content

1967 ◽  
Vol 26 (5) ◽  
pp. 1078-1081 ◽  
Author(s):  
R. W. Benson ◽  
B. W. Pickett ◽  
R. J. Komarek ◽  
J. J. Lucas
Keyword(s):  
Lipid Content ◽  
Cold Shock ◽  
Boar Spermatozoa

In vitro phagocytosis of boar spermatozoa by neutrophils from peripheral blood of sows

Reproduction ◽  
2000 ◽  
pp. 265-273 ◽  
Author(s):  
A Matthijs ◽  
W Harkema ◽  
B Engel ◽  
H Woelders
Keyword(s):  
Cold Shock ◽  
Female Genital Tract ◽  
Polymorphonuclear Leucocytes ◽  
Freezing And Thawing ◽  
Phagocytosis Assay ◽  
Acrosomal Exocytosis ◽  
Boar Spermatozoa ◽  
Female Genital ◽  
Serum Components

A considerable number of spermatozoa are used in each sow in routine artificial insemination. However, within a few hours after insemination, many spermatozoa are phagocytosed by polymorphonuclear leucocytes. Some aspects of sperm transport in the female genital tract in the sow have been thoroughly investigated, whereas little is known about the mechanisms involved in the phagocytosis of spermatozoa, or about which spermatozoa (fresh, capacitated or dead) are the most susceptible to ingestion by polymorphonuclear leucocytes. In this study, phagocytosis was investigated by use of an in vitro phagocytosis assay. Polymorphonuclear leucocytes were challenged with either untreated, cold-shocked or frozen-thawed spermatozoa, or with spermatozoa that had been treated to induce capacitation in vitro. The influence of serum on phagocytosis was also investigated. Treatment of the semen to induce capacitation in vitro considerably reduced the phagocytosis of spermatozoa, whereas crude treatments like cold-shock or freezing and thawing reduced phagocytosis only in the first 15-30 min of incubation with polymorphonuclear leucocytes. Viable spermatozoa were phagocytosed mainly through a pathway that was independent of complement or other serum components (for example, antibodies). Complement had little effect on phagocytosis of spermatozoa, but did cause acrosomal exocytosis and cell death.

scholarly journals Properties of spermatozoal superoxide dismutase and lack of involvement of superoxides in metal-ion-catalysed lipid-peroxidation and reactions in semen

1980 ◽  
Vol 191 (2) ◽  
pp. 289-297 ◽  
Author(s):  
M R F Mennella ◽  
R Jones
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Metal Ion ◽  
Cold Shock ◽  
Total Activity ◽  
Cellular Damage ◽  
Purification And Characterization ◽  
Boar Spermatozoa

1. The distribution and properties of superoxide dismutase were examined in mammalian semen, and the enzyme was used to investigate the role of superoxides in metal-ion-catalysed lipid-peroxidation reactions in spermatozoa. 2. Superoxide dismutase activity was detected in seminal plasma and spermatozoa from all species studied, exceptionally high activity being found in donkey semen. The enzyme is easily solubilized from spermatozoa, as 85-90% of the total activity is released by cold shock, a relatively mild form of cellular damage. 3. Purification and characterization of the enzyme from supernatant fractions prepared from cold-shocked boar spermatozoa showed it to be cyanide-sensitive, to have a mol.wt. of 31 000, a pI of 5.9 and to contain 1.85 g-atoms of copper and 1.91 g-atoms of zinc per mol of protein. However, extensive sonication of spermatozoa released a small amount of a cyanide-insensitive enzyme, presumably a mangano superoxide dismutase, from the mitochondrial matrix. 4. The presence of superoxide dismutase in spermatozoa, either intracellularly or extracellularly, did not inhibit ascorbate/Fe2+-catalysed lipid-peroxidation reactions, suggesting that superoxides are not essential intermediates in this system.

scholarly journals The Effect of Cold Shock and Freeze-Thawing on Release of Phospholipids by Ram, Bull, and Boar Spermatozoa

1973 ◽  
Vol 26 (6) ◽  
pp. 1409 ◽  
Author(s):  
Annabelle Darin-Bennett ◽  
A Poulos ◽  
IG White
Keyword(s):  
Phosphatidyl Choline ◽  
Species Differences ◽  
Cold Shock ◽  
Cell Structure ◽  
Temperature Treatment ◽  
Absolute Amount ◽  
The Absolute ◽  
Boar Spermatozoa

The effects of cold shock and freeze-thawing on the release of total phospholipids phosphorus and of specific phospholipids from ram, bull, and boar spermatozoa are examined. Species differences are apparent, both in the absolute amount of total phospholipids released and in the conditions required to effect a loss of individual phospholipids. The phosphoglycerides most affected by the temperature treatment are choline plasmalogen, phosphatidyl choline, and phosphat idyl ethanolamine. The loss of phospholipids is most specific in boar spermatozoa. Bull spermatozoa suffer a greater overall and more general breakdown of phospholipids, indicating disruption of the cell structure. This is in contrast to the more specific phospholipid losses from ram and boar spermatozoa, which may indicate a more localized region of damage and release of material, possibly acrosomal. The loss of phospholipid may be correlated with the species differences in the fertility of the frozen spermatozoa.

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