scholarly journals Effect of cold preservation on intracellular calcium concentration and calpain activity in rat sinusoidal endothelial cells

Hepatology ◽  
2003 ◽  
Vol 37 (2) ◽  
pp. 313-323 ◽  
Author(s):  
G Upadhya
Keyword(s):  
Endothelial Cells ◽  
Intracellular Calcium ◽  
Calcium Concentration ◽  
Intracellular Calcium Concentration ◽  
Calpain Activity ◽  
Sinusoidal Endothelial Cells ◽  
Cold Preservation

Effect of hypoxia upon intracellular calcium concentration of human endothelial cells

1992 ◽  
Vol 152 (1) ◽  
pp. 215-221 ◽  
Author(s):  
Thierry Arnould ◽  
Carine Michiels ◽  
Isabelle Alexandre ◽  
Jos� Remacle
Keyword(s):  
Endothelial Cells ◽  
Intracellular Calcium ◽  
Calcium Concentration ◽  
Intracellular Calcium Concentration ◽  
Human Endothelial Cells

Heterogeneous response of microvascular endothelial cells to shear stress

2006 ◽  
Vol 290 (6) ◽  
pp. H2498-H2508 ◽  
Author(s):  
D. Hong ◽  
D. Jaron ◽  
D. G. Buerk ◽  
K. A. Barbee
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Intracellular Calcium ◽  
Purinergic Receptors ◽  
Calcium Concentration ◽  
Intracellular Calcium Concentration ◽  
Calcium Signal ◽  
Calcium Waves ◽  
Aortic Endothelial Cells ◽  
Atp Concentration

We investigated changes in calcium concentration in cultured bovine aortic endothelial cells (BAECs) and rat adrenomedulary endothelial cells (RAMECs, microvascular) in response to different levels of shear stress. In BAECs, the onset of shear stress elicited a transient increase in intracellular calcium concentration that was spatially uniform, synchronous, and dose dependent. In contrast, the response of RAMECs was heterogeneous in time and space. Shear stress induced calcium waves that originated from one or several cells and propagated to neighboring cells. The number and size of the responding groups of cells did not depend on the magnitude of shear stress or the magnitude of the calcium change in the responding cells. The initiation and the propagation of calcium waves in RAMECs were significantly suppressed under conditions in which either purinergic receptors were blocked by suramin or extracellular ATP was degraded by apyrase. Exogenously applied ATP produced similarly heterogeneous responses. The number of responding cells was dependent on ATP concentration, but the magnitude of the calcium change was not. Our data suggest that shear stress stimulates RAMECs to release ATP, causing the increase in intracellular calcium concentration via purinergic receptors in cells that are heterogeneously sensitive to ATP. The propagation of the calcium signal is also mediated by ATP, and the spatial pattern suggests a locally elevated ATP concentration in the vicinity of the initially responding cells.

scholarly journals The effect of the calcium-antagonist nitrendipine on intracellular calcium concentration in endothelial cells

1996 ◽  
Vol 118 (8) ◽  
pp. 1899-1904 ◽  
Author(s):  
Aida Salameh ◽  
Gunhild Schomecker ◽  
Katja Breitkopf ◽  
Stefan Dhein ◽  
Wolfgang Klaus
Keyword(s):  
Endothelial Cells ◽  
Calcium Antagonist ◽  
Intracellular Calcium ◽  
Calcium Concentration ◽  
Intracellular Calcium Concentration
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