Antifungal activity of plant essential oils against wild strains of Candida spp. isolated from hospitalized patients

Abstract The aims of this research were: evaluate the chemical composition and the cytotoxicity of the Cuminum cyminum (cumin), Anethum graveolens (dill), Pimpinella anisum (anise) and Foeniculum vulgare (fennel) essential oils, as well as their antifungal activity in vitro against ten Candida spp. isolates. The chemical composition of the oils was analyzed by means of gas chromatography coupled with mass spectrometry (GC/MS). The cytotoxicity assays were performed, using the cell proliferation reagent WST-1 in L929 mouse fibroblasts (20x103 well-1). The determinate the Minimum Inhibitory Concentration (MIC), was performed through the Broth Microdilution technique (CLSI). The chemical main components were the cuminaldehyde (32.66%) for cumin, carvone (34.89%) for the dill, trans-anethole (94.01%) for the anise and anethole (79.62%) for the fennel. Anise and fennel did not were cytotoxic in all the tested concentrations, however the cumin oil was cytotoxic in the concentration of 20 mg.mL-1 and the dill in the concentrations of 20 and 8 mg.mL-1. All yeasts were susceptible against the evaluated essential oils. Cumin presented the lowest MIC against yeasts. We concluded that all the essential oils presented inhibitory action against Candida spp., and C . cyminum, P. anisum and F. vulgare were not cytotoxic in the same minimum inhibitory concentrations for the fungi.

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In vitro Antiproliferative and Antifungal Activity of Essential Oils from Erigeron acris L. and Erigeron annuus (L.) Pers.

Zeitschrift für Naturforschung C ◽

10.1515/znc-2010-11-1202 ◽

2010 ◽

Vol 65 (11-12) ◽

pp. 642-646 ◽

Cited By ~ 9

Author(s):

Jolanta Nazaruk ◽

Ewa Karna ◽

Piotr Wieczorek ◽

Paweł Sacha ◽

Elżbieta Tryniszewska

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

Antiproliferative Activity ◽

Endometrial Adenocarcinoma ◽

Candida Spp ◽

Cell Viability Assay ◽

Erigeron Annuus ◽

Mcf 7

Antiproliferative and antifungal activities of essential oils from Erigeron acris root and herb and from Erigeron annuus herb were investigated. The cell viability assay was performed in cultured fi broblasts, cancer cell lines (MCF-7 and MDA-MBA-231), and endometrial adenocarcinoma (Ishikawa) cells as well as colon adenocarcinoma (DLD-1) cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The essential oil from E. acris root showed the highest antiproliferative activity in the MCF-7 cell line with an IC50 value of 14.5 μg/mL. No effect of the essential oil on normal cells at that concentration was found. Antifungal activity against various strains of five Candida species, i.e. C. albicans, C. glabrata, C. tropicalis, C. krusei, and C. parapsilosis, was tested by the microdilution method. It was found that all examined oils can be useful as antifungal agents against the abovementioned species, but the essential oil of E. acris herb was the most active. Their minimum inhibitory concentrations (MIC) ranged from 30 to 0.4 μL/mL. The data presented suggest that essential oils from E. acris and E. annuus possess antifungal activity against Candida spp. and antiproliferative activity against breast cancer MCF-7 cells

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Synergistic Activity of New Diclofenac and Essential Oils Combinations against Different Candida spp.

Antibiotics ◽

10.3390/antibiotics10060688 ◽

2021 ◽

Vol 10 (6) ◽

pp. 688

Author(s):

Antonio Rosato ◽

Elisabetta Altini ◽

Sabina Sblano ◽

Lara Salvagno ◽

Filippo Maggi ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oils ◽

Diclofenac Sodium ◽

Synergistic Activity ◽

Candida Spp ◽

Pelargonium Graveolens ◽

Mentha X Piperita ◽

Inflammatory Drugs ◽

Antimicrobial And Antifungal Activity

According to recent studies, Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) have shown a good antimicrobial and antifungal activity. Their association with essential oils (EOs) could be useful for the treatment of infections caused by Candida spp. The aim of this studyis to evaluate the synergistic antifungal activity of new combinations between Diclofenac Sodium Salt (DSS), a widely used NSAID, with EOs of Mentha x piperita, Pelargonium graveolens and Melaleuca alternifolia. The in-vitroantifungal activity was determined on different Candida strains. The determination of the chemical composition of EOs was carried out by gaschromatography-massspectrometry (GC-MS). Susceptibility testing of planktonic cells was performed by using the broth microdilution assay and checkerboard methods. Minimum Inhibitory Concentrations (MIC) of DSS was in a range from 1.02 to 2.05 mg/mL reaching a MIC value of 0.05 mg/mL when combined with Pelargonium graveolens (FICI= 0.23–0.35) or Menthapiperita (FICI= 0.22–0.30) EOs. These preliminary results show thatthe combination of the EOs with DSS improves the antifungal activity on all the tested Candida strains.

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Antifungal activity of epecific plant essential oils against fusarium graminearum

Journal of Plant Science and Phytopathology ◽

10.29328/journal.jpsp.1001052 ◽

2020 ◽

Vol 4 (3) ◽

pp. 060-062

Author(s):

Özsoy Esma ◽

Kesercan Buket ◽

Yörük Emre

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

Fusarium Graminearum ◽

Management Strategies ◽

Head Blight ◽

Fungicide Treatment ◽

Plant Essential Oils ◽

Cultivar Development ◽

Potential Use

fusarium graminearum is one of the most popular phytopathogens of cereals worldwide. F. graminearum is the major causal agent of head blight of wheat and barley. Disease-resistant cultivar development, antagonistic microorganism usage and fungicide treatment are the most common strategies in head blight management strategies. However, these methods have some important disadvantages. The use of plant-derived essential oil against F. graminearum seems to be a promising approach due to the recent researches. This review summarizes the potential use of essential oils to fight against F. graminearum.

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Antifungal activity of six plant essential oils from Serbia against Trichoderma aggressivum f. europaeum

Pesticidi i fitomedicina ◽

10.2298/pif1404291d ◽

2014 ◽

Vol 29 (4) ◽

pp. 291-297 ◽

Cited By ~ 2

Author(s):

Rada Djurovic-Pejcev ◽

Ivana Potocnik ◽

Svetlana Milijasevic-Marcic ◽

Biljana Todorovic ◽

Emil Rekanovic ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oils ◽

Juglans Regia ◽

Mentha Piperita ◽

Button Mushroom ◽

Minimum Fungicidal Concentration ◽

Plant Essential Oils ◽

Hypericum Perforatum L ◽

Green Mould ◽

Main Components

Six essential oils (EOs) extracted from plants originating in Serbia were assayed for inhibitory and fungicidal activity against a major fungal pathogen of button mushroom causing green mould disease, Trichoderma agressivum f. europaeum. The strongest activity was demonstrated by the oils of basil (Ocimum basilicum L.) and peppermint (Mentha piperita L.). Medium antifungal activity of St. John's wort (Hypericum perforatum L.) and walnut [Juglans regia (F)] oils was also recorded. Oils extracted from yarrow (Achillea millepholium L.) and juniper (Juniperus communis L.) exhibited the lowest activity. Peppermint oil showed fungicidal effect on the pathogen, having a minimum fungicidal concentration of 0.64 ?l ml-1. The main components of peppermint essential oil were menthone (37.02%), menthol (29.57%) and isomenthone (9.06%).

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In vitro activity of essential oils extracted from plants used as spices against fluconazole-resistant and fluconazole-susceptible Candida spp.

Canadian Journal of Microbiology ◽

10.1139/w08-097 ◽

2008 ◽

Vol 54 (11) ◽

pp. 950-956 ◽

Cited By ~ 53

Author(s):

Patrícia Pozzatti ◽

Liliane Alves Scheid ◽

Tatiana Borba Spader ◽

Margareth Linde Atayde ◽

Janio Morais Santurio ◽

...

Keyword(s):

Chemical Composition ◽

Antifungal Activity ◽

Essential Oils ◽

Zingiber Officinale ◽

Retention Indices ◽

Thymus Vulgaris ◽

Candida Spp ◽

Fluconazole Resistant ◽

Rosmarinus Officinalis L

In the present study, the antifungal activity of selected essential oils obtained from plants used as spices was evaluated against both fluconazole-resistant and fluconazole-susceptible Candida spp. The Candida species studied were Candida albicans , Candida dubliniensis , Candida tropicalis , Candida glabrata , and Candida krusei. For comparison purposes, they were arranged in groups as C. albicans, C. dubliniensis, and Candida non-albicans. The essential oils were obtained from Cinnamomum zeylanicum Breyn, Lippia graveolens HBK, Ocimum basilicum L., Origanum vulgare L., Rosmarinus officinalis L., Salvia officinalis L., Thymus vulgaris L., and Zingiber officinale . The susceptibility tests were based on the M27-A2 methodology. The chemical composition of the essential oils was obtained by gas chromatography – mass spectroscopy and by retention indices. The results showed that cinnamon, Mexican oregano, oregano, thyme, and ginger essential oils have different levels of antifungal activity. Oregano and ginger essential oils were found to be the most and the least efficient, respectively. The main finding was that the susceptibilities of fluconazole-resistant C. albicans, C. dubliniensis, and Candida non-albicans to Mexican oregano, oregano, thyme, and ginger essential oils were higher than those of the fluconazole-susceptible yeasts (P < 0.05). In contrast, fluconazole-resistant C. albicans and Candida non-albicans were less susceptible to cinnamon essential oil than their fluconazole-susceptible counterparts (P < 0.05). A relationship between the yeasts’ susceptibilities and the chemical composition of the essential oils studied was apparent when these 2 parameters were compared. Finally, basil, rosemary, and sage essential oils did not show antifungal activity against Candida isolates at the tested concentrations.

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