Role of thin layer chromatography in detection of antibacterial activity of essential oils

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
G Horváth ◽  
K Ács ◽  
W Jesionek ◽  
I Choma ◽  
A Böszörményi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Thin Layer ◽  
Essential Oils ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF BIXIN PIGMENT FROM ANNATTO (Bixa orellana L.) SEEDS

2010 ◽  
Vol 7 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Pipin T. Kurniawati ◽  
H. Soetjipto ◽  
Leenawati Limantara
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bixa Orellana ◽  
Isolation And Identification ◽  
Double Beam ◽  
Layer Chromatography

Research on Bixa orellana L. have been done to isolate, identify and determine bixin percentage, the antioxidant and antibacterial activities of bixin from B. orellana seed.  Isolation and identification of bixin was done by thin layer chromatography (TLC), column chromatography, chemical test of bixin and UV-Vis double beam spectroscopy. Percentage of bixin was calculated by JECFA method, the antioxidant activity was determined by DPPH (1-1 diphynilpicrylhidrazil) method while antibacterial activity was analyzed by the use of agar diffusion method. Thin layer chromatography (TLC) for the crude extract contained 5 spot, where spot 5th was bixin. Bixa orellana has 75±3% of bixin. Antioxidant activity of bixin had IC50 548.5±20.0 ppm. Whereas the antibacterial activity of bixin against the Escherichia coli and Staphylococus aureus could be classified as weak inhibition category at 500-750 μg and medium inhibition category at 1500 μg.   Keywords: Bixa orellana L., bixin, antioxidant, antibacteria

Role of the solvent in thin-layer chromatography of steroids on alumina

1984 ◽  
Vol 18 (1) ◽  
pp. 37-40 ◽  
Author(s):  
S. M. Petrović ◽  
L. A. Kolarov ◽  
Julijana A. Petrović
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

scholarly journals KAJIAN FRAKSI METANOL DARI EKSTRAK METILEN DIKLORIDA KULIT KAYU BATANG PELAWAN (Tristania whitiana Griff.) SEBAGAI ANTIBAKTERI

Molekul ◽  
2007 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Purwantiningsih Sugita
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Inhibitory Concentration ◽  
Methanol Fraction ◽  
The Third ◽  
The One ◽  
Layer Chromatography ◽  
Chloroform Methanol

Antibacterial compound from methanol fraction of methylene dichloride extract of pelawan bark had been studied. Pelawan bark was extracted using ethanol 95% and CH2Cl2-water (1:1), respectively. Extract of CH2Cl2 was partitioned using hexane-methanol 90% (1:1). Afterwards, methanol fraction was analyzed by thin layer chromatography methods to find the best eluent for column chromatography. The best eluent came from mixture of acetone-chloroform-methanol (0,6:4:0,4). The fractionation yielded 16 fractions with the first (M1) and the third (M5) fractions became the one spotted fractions. M1 and M5 fractions were examined for their antibacterial activity using paper diffusion methods by determining minimum inhibitory concentration value. M1 fraction showed greater activity than M5 fraction with minimum inhibitory concentration value of 2,7857 mg/mL. The result of phytochemistry test showed that both fractions contain terpenoid.

Some Considerations on the Role of Water in Thin Layer Chromatography of Inorganic Anions

1984 ◽  
Vol 39 (2) ◽  
pp. 186-189
Author(s):  
Takaya Iida ◽  
Kaname Ito
Keyword(s):  
Thin Layer ◽  
Partition Coefficient ◽  
Thin Layer Chromatography ◽  
Partition Coefficients ◽  
Ionic Radius ◽  
Stationary Phases ◽  
Partial Charge ◽  
Layer Chromatography ◽  
Water Contents

The volume ratios of the mobile and stationary phases in thin layer chromatography, and the partition coefficients of halide and halate anions and some other anions between these phases were obtained by developing the ions simultaneously with a series of metaphosphates. The water contents of the developing solvents influenced the volume ratios very much, but not so much the partition coefficients. The logarithm of the partition coefficient of the halide anions is proportional to the reciprocal of their ionic radius, and that of the halate anions and oxoanions to the partial charge on the oxygens of them.

Purification of vetiver alcohols and esters for quantitative high-performance thin-layer chromatography determination in Haitian vetiver essential oils and vetiver acetates

2012 ◽  
Vol 1241 ◽  
pp. 103-111 ◽  
Author(s):  
Lionel Paillat ◽  
Christine Périchet ◽  
Jean-Philippe Pierrat ◽  
Sophie Lavoine ◽  
Jean-Jacques Filippi ◽  
...  
Keyword(s):  
Thin Layer ◽  
Essential Oils ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Layer Chromatography
Sign in / Sign up

Export Citation Format

Share Document