Correlation between the Expression of Interleukin-6, STAT3, E-Cadherin and N-Cadherin Protein and Invasiveness in Nonfunctional Pituitary Adenomas

2019 ◽  
Author(s):  
Xiaoxu Shen ◽  
Qi Liu ◽  
Jian Xu ◽  
Yang Wang
Keyword(s):  
Pituitary Adenoma ◽  
Correlation Analysis ◽  
Pituitary Adenomas ◽  
Signal Transducer ◽  
Epithelial Cadherin ◽  
Nonfunctional Pituitary Adenomas ◽  
Transcription 3 ◽  
Spearman’S Correlation ◽  
E Cadherin

Abstract Objective This study aimed to investigate the expression of interleukin (IL)-6, signal transducer and activator of transcription 3 (STAT3), epithelial-cadherin (E- cadherin) and neural-cadherin (N-cadherin) proteins in nonfunctional pituitary adenomas, and their correlation with invasiveness. Method Thirty cases of nonfunctional pituitary adenoma pathological wax specimens were selected from our hospital, including 20 cases of invasive nonfunctional pituitary adenoma (INFPA) and 10 noninvasive nonfunctional pituitary adenomas (NNFPAs). Envision was used to detect IL-6, STAT3, E-cadherin , and N-cadherin in specimens. Statistical methods were used to analyze the correlation between the four proteins and the Knosp classification of nonfunctional pituitary adenomas. Result IL-6 and STAT3 were highly expressed in INFPAs but poorly expressed in NNFPAs. E-cadherin expression in INFPAs was lower than that in NNFPAs. N-cadherin was positive or strongly positive in both groups. Spearman's correlation analysis showed that the expression of IL-6 and STAT3 was positively correlated with Knosp's classification, whereas the expression of E-cadherin was negatively correlated with Knosp classification. Meanwhile, the expression of N-cadherin was not correlated with Knosp's classification. Conclusion The expression of the IL-6, STAT3, E-cadherin proteins were associated nonfunctional pituitary adenomas. However, the expression of N-cadherin was not correlated with nonfunctional pituitary adenomas.

scholarly journals Emerging role of signal transducer and activator of transcription 3 (STAT3) in pituitary adenomas

2021 ◽  
Author(s):  
Cyndy Liu ◽  
Tae Nakano-Tateno ◽  
Motoyasu Satou ◽  
Constance Chik ◽  
Toru Tateno
Keyword(s):  
Pituitary Adenomas ◽  
Signal Transducer ◽  
Transcription 3

scholarly journals Signal transducer and activator of transcription 3 (STAT3) promoter methylation and expression in pituitary adenoma

2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Indre Valiulyte ◽  
Giedrius Steponaitis ◽  
Daina Skiriute ◽  
Arimantas Tamasauskas ◽  
Paulina Vaitkiene
Keyword(s):  
Pituitary Adenoma ◽  
Promoter Methylation ◽  
Signal Transducer ◽  
Transcription 3

scholarly journals Constitutively Activated Stat3 Induces Tumorigenesis and Enhances Cell Motility of Prostate Epithelial Cells through Integrin β6

2007 ◽  
Vol 27 (12) ◽  
pp. 4444-4453 ◽  
Author(s):  
Janeen Azare ◽  
Kenneth Leslie ◽  
Hikmat Al-Ahmadie ◽  
William Gerald ◽  
Paul H. Weinreb ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Soft Agar ◽  
Cellular Migration ◽  
Mutant Form ◽  
Signal Transducer ◽  
Prostate Tumorigenesis ◽  
Prostate Epithelial Cells ◽  
Integrin Αvβ6 ◽  
Transcription 3 ◽  
E Cadherin

ABSTRACT The persistent activation of signal transducer and activator of transcription 3 (Stat3) is a common feature of prostate cancer. However, little is known about the Stat3 targets that may mediate prostate tumorigenesis. The introduction of an activating mutant form of Stat3 (Stat3-C) into immortalized prostate epithelial cells resulted in tumorigenesis. Stat3-C-expressing cells had decreased E-cadherin levels, increased numbers of lamellipodia and stress fibers, and enhanced migratory capacities compared to vector control-expressing cells, with a concomitant increase in the expression of integrin β6 and its ligand, fibronectin (FN). Exogenously added FN increased cellular migration, with a concomitant loss of E-cadherin expression. The blockade of integrin αvβ6 in Stat3-C-expressing cells inhibited migration, increased E-cadherin levels, and reduced colony formation in soft agar. These results demonstrate the sufficiency of constitutively activated Stat3 in mediating prostate tumorigenesis and identify novel Stat3 targets that are involved in promoting cell migration and transformation.

scholarly journals CCNB1 affects cavernous sinus invasion in pituitary adenomas through the epithelial–mesenchymal transition

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Bin Li ◽  
Jianhua Cheng ◽  
Hongyun Wang ◽  
Sida Zhao ◽  
Haibo Zhu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pituitary Adenoma ◽  
Cell Lines ◽  
Cavernous Sinus ◽  
Pituitary Adenomas ◽  
Epithelial Mesenchymal Transition ◽  
Mesenchymal Transition ◽  
Cavernous Sinus Invasion ◽  
The Relationship ◽  
E Cadherin

Abstract Background To investigate the relationship between cyclin B1 (CCNB1) gene expression and cavernous sinus invasion in pituitary adenomas. Methods Twenty-four pituitary adenoma tissue samples were examined by RT-qPCR and Western blot to assess the mRNA expression levels and protein levels of CCNB1, E-cadherin and N-cadherin. Correlation analyses between the expression levels of E-cadherin, N-cadherin and CCNB1 were performed. After lentivirus-mediated knockdown of CCNB1 in rat pituitary adenoma cell lines (GH3 and GT1-1), cell function changes were studied. The relationship between CCNB1 and epithelial-mesenchymal transition (EMT) was further verified by animal experiments. Results CCNB1 and N-cadherin gene expression were significantly higher in the invasive pituitary adenomas than in the non-invasive pituitary adenomas. Conversely, E-cadherin expression in the invasive pituitary adenomas was significantly lower. CCNB1 gene expression was downregulated in the GH3 and GT1-1 pituitary adenoma cell lines; N-cadherin expression was also decreased, but E-cadherin expression was increased. These results were confirmed in vivo. After downregulation of CCNB1, cell invasion and migration was significantly reduced in Transwell experiments. Conclusion High CCNB1 expression in pituitary adenoma affects cavernous sinus invasion through EMT.

Hypoxia is a potent inducer of the iron masterswitch hepcidin via Signal Transducer and Activator of Transcription 3 (STAT3)

2017 ◽  
Author(s):  
I Silva ◽  
V Rausch ◽  
T Peccerella ◽  
G Millonig ◽  
HK Seitz ◽  
...  
Keyword(s):  
Signal Transducer ◽  
Potent Inducer ◽  
Transcription 3

Molecular classification of pituitary adenomas

2015 ◽  
Author(s):  
Antonio Pico ◽  
Laura Sanchez-Tejada ◽  
Ruth Sanchez-Ortiga ◽  
Rosa Camara ◽  
Cristina Lamas ◽  
...  
Keyword(s):  
Pituitary Adenomas ◽  
Molecular Classification
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