In Vitro activity of a Novel Benzoquinolizine Antibiotic, Levonadifloxacin (WCK 771) against Blood Stream Gram-Positive Isolates from a Tertiary Care Hospital

Abstract Background Blood stream infections (BSIs) due to Gram-positive pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) are associated with high mortality ranging from 10 to 60%. The current anti-MRSA agents have limitations with regards to safety and tolerability profile which limits their prolonged usage. Levonadifloxacin and its oral prodrug alalevonadifloxacin, a novel benzoquinolizine antibiotic, have recently been approved for acute bacterial skin and skin structure infections including diabetic foot infections and concurrent bacteremia in India. Methods The present study assessed the potency of levonadifloxacin, a novel benzoquinolizine antibiotic, against Gram-positive blood stream clinical isolates (n = 31) collected from January to June 2019 at a tertiary care hospital in Mumbai, India. The susceptibility of isolates to antibacterial agents was defined following the Clinical and Laboratory Standard Institute interpretive criteria (M100 E29). Results High prevalence of MRSA (62.5%), quinolone-resistant Staphylococcus aureus (QRSA) (87.5%), and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) (82.35%) were observed among bacteremic isolates. Levonadifloxacin demonstrated potent activity against MRSA, QRSA, and MR-CoNS strains with significantly lower minimum inhibitory concentration MIC50/90 values of 0.5/1 mg/L as compared with levofloxacin (8/32 mg/L) and moxifloxacin (2/8 mg/L). Conclusion Potent bactericidal activity coupled with low MICs support usage of levonadifloxacin for the management of BSIs caused by multidrug resistant Gram-positive bacteria.

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In-vitro susceptibility of linezolid against methicillin resistant Staphylococcus aureus at a tertiary care hospital in Pakistan

Journal of Microbiology and Infectious Diseases ◽

10.5799/ahinjs.02.2013.04.0109 ◽

2013 ◽

Vol 03 (04) ◽

pp. 203-206 ◽

Cited By ~ 1

Author(s):

Ali Khalid

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Care Hospital ◽

In Vitro Susceptibility ◽

Methicillin Resistant

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Correlation between Vancomycin MIC Values and Those of Other Agents against Gram-Positive Bacteria among Patients with Bloodstream Infections Caused by Methicillin-Resistant Staphylococcus aureus

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00307-09 ◽

2009 ◽

Vol 53 (12) ◽

pp. 5141-5144 ◽

Cited By ~ 19

Author(s):

N. Patel ◽

P. Lubanski ◽

S. Ferro ◽

M. Bonafede ◽

S. Harrington ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Bloodstream Infections ◽

Care Hospital ◽

Gram Positive ◽

Methicillin Resistant ◽

Gram Positive Bacteria ◽

Included Patient

ABSTRACT An increase in the distribution of vancomycin MIC values among methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) isolates has been noted. It is postulated that the shift in vancomycin MIC values may be associated with a concurrent rise in the MIC values of other anti-MRSA agents. Scant data are available on the correlation between vancomycin MIC values and the MIC values of other anti-MRSA agents. This study examined the correlation between vancomycin MIC values and the MIC values of daptomycin, linezolid, tigecycline, and teicoplanin among 120 patients with bloodstream infections caused by MRSA at a tertiary care hospital between January 2005 and May 2007. For each included patient, the MIC values of the antibiotics under study were determined by the Etest method and were separated into the following two categories: day 1 (index) and post-day 1 (subsequent). For subsequent isolates, the MIC values for each antibiotic from the post-day 1 terminal isolate were used. Among the index isolates, there was a significant correlation (P value, <0.01) between the MIC values for vancomycin and daptomycin and between the MIC values for vancomycin and teicoplanin. The MIC values for daptomycin were significantly correlated with linezolid, tigecycline, and teicoplanin MIC values. Among the 48 patients with subsequent isolates, vancomycin MIC values were significantly correlated with MIC values for daptomycin, linezolid, and teicoplanin (ρ value of ≥0.38 for all comparisons). This study documented an association between vancomycin MIC values and the MIC values of other anti-MRSA antibiotics among patients with bloodstream infections caused by MRSA primarily treated with vancomycin.

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Prevalence, Risk factors and Antibiogram analysis of Nosocomial Infection in Tertiary Care Hospital of Rawalpindi, Pakistan

10.21203/rs.3.rs-145858/v1 ◽

2021 ◽

Author(s):

Jadoon Khan ◽

Israr Ahmad ◽

Nosheen Basharat ◽

Alam Khan ◽

Asma Sadiq ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Nosocomial Infection ◽

Nosocomial Infections ◽

Methicillin Resistant Staphylococcus Aureus ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Care Hospital ◽

Gram Positive ◽

Gram Negative ◽

Methicillin Resistant

Abstract BackgroundNosocomial infections are a global health problem, affecting 1.4 million people in treatment centers, responsible for 80,000 estimated annual deaths. The current study aimed to assess the factor influencing nosocomial infections and to choose the best antibiotic for its treatment through culture analysis.MethodologyThe current study was conducted in a tertiary care hospital Rawalpindi. A total of 120 patients with at least one nosocomial episode were included. Blood, urine, and wound swab sample were collected for hematology, biochemistry, electrolyte, and microbial analysis. Multivariate regression analysis through SPSS (ver. 16.0) were done, p-value of ≤ 0.05 was considered statistically significant.ResultThe prevalence of culture-confirmed nosocomial infection was 25%, among which age groups (P = 0.00, 95% CI, − .382-.271) were significantly correlated. Hematological analysis shows that 73.3% have lymphopenia (P = 0.00, 95% CI .567-1.175), 73.3% have Neutrocytosis (P = 0.00, 95% CI .553-1.122) and anemia (26.7%) (P = 0.002, 95% CI .097-.404) were statistically significant. Raised level of ALT (60%), Bilirubin (26.7%) and ALP (13.3%) among liver functional tests (P = 0.68, 95% CI .686- .280) found insignificant however abnormal level of urea (33.3%) and creatinine (46.7%) (P = 0.00, 95% CI -1.227-.392) were significantly correlated with nosocomial infections. Electrolytes profile shows that Hypernatremia (26.7%) (P = 0.000, 95% CI-.491–.227) were strongly correlated. Culture analysis isolated six bacterial agents, comprising 83.3%:16.6% ratio of gram-negative and gram-positive isolates. Klebsiella pneumonia was frequently isolated gram-negative, while Methicillin-Resistant Staphylococcus aureus was the only gram-positive isolate collected. Urinary tract infection (UTI) (36.6%) was frequently found, followed by bloodstream infection (26.6%) (BSI). The majority of the gram-negative isolates were sensitive to Imipenem while resistant to Amoxicillin + Clavulanic acid, Trimethoprim/sulfamethoxazole, cefoxitin, Levofloxacin, Norfloxacin, and linezolid antibiotics. Methicillin-resistant staphylococcus aureus was found sensitive against Trimethoprim/sulfamethoxazole while resistant towards linezolid, Imipenem, and Cefotaxime.ConclusionThe current study revealed that nosocomial infection is still prevalent in our hospital environment and the leading cause of drug resistance and dysfunctions of various factors like WBCs, LFTs, RFTs, electrolytes, coagulation factors and anemia, which can lead to morbidity and mortality.

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