Evaluation of Serum Immunoglobulins (IgG, IgA, IgM) and Circulating Immune Complexes in Oral Precancer and Cancer Patients

Abstract Objectives The aim of the present study is to evaluate the role of immunoglobulins (IgA, IgG, and IgM) and circulating immune complexes (CIC) as tumor marker in oral cancer and precancer patients. Materials and Methods The present study was performed on 45 individuals subdivided into three groups, that is, oral precancer, oral cancer and healthy individuals, and levels of immunoglobulins, and CIC was estimated by turbidometry and ELISA method. Results In the present study, the mean serum IgA levels in oral precancer were 161.00 ( ± 118.02) mg/dL, oral cancers were 270.67 ( ± 171.44) mg/dL, and controls were 133.73 ( ± 101.31) mg/dL. Mean serum levels of IgG in oral precancer were 1,430.87 ( ± 316) mg/dL, oral cancers were 1,234.27 ( ± 365.42) mg/dL, and controls were 593.87 ( ± 323.06) mg/dL. Conclusion We found that the levels of serum IgG and IgA were elevated consistently in precancer and cancer group, and Serum IgM levels were increased only in precancer. Also, significant increase in serum CIC levels were seen in oral precancer and cancer group on comparison with control.

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Accumulation of antinuclear associated antibodies in circulating immune complexes is more prominent in SLE patients from Sudan than Sweden

Scientific Reports ◽

10.1038/s41598-020-78213-5 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Sahwa Elbagir ◽

Azita Sohrabian ◽

Amir I. Elshafie ◽

Elnour M. Elagib ◽

Nasr Eldeen A. Mohammed ◽

...

Keyword(s):

Immune Complexes ◽

Disease Duration ◽

Serum Levels ◽

Circulating Immune Complexes ◽

Limited Extent ◽

Sledai Score ◽

Acr Criteria ◽

Black Populations ◽

Ribosomal P

AbstractThe role of anti-nuclear autoantibody (ANA) specificities in immune complexes (IC) formation has been studied to a limited extent in SLE, and not at all in African SLE patients. We compared ANA in IC from Sudanese and Swedish SLE patients. We included 93 Sudanese and 332 Swedish SLE patients fulfilling the 1982 ACR criteria. IC were captured using C1q-coated beads. ANA specificities were quantified in sera and IC. Results were related to modified SLEDAI. Whereas serum levels of anti-Sm, anti-dsDNA and anti-ribosomal P were higher in Swedish patients, IC levels of most ANA specificities were higher among Sudanese patients. This difference was especially prominent for anti-chromatin antibodies, which remained after adjustment for age, disease duration and treatment. Total levels of C1q-binding IC correlated with levels of specific ANA in IC, with highest correlations for anti-chromatin antibodies among Sudanese patients. Whereas occurrence of anti- SSA/Ro60, anti-histone and anti-U1RNP in both serum and IC associated with high SLEDAI score, anti-dsDNA in IC but not in serum associated with high SLEDAI. ANA, especially antibodies targeting chromatin, accumulate more in IC from Sudanese SLE patients. If the autoantibody fraction forming IC is pathogenically important, this might explain the generally described severe SLE in black populations.

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Role of Circulating Immune Complexes in the Pathogenesis of Canine Leishmaniasis: New Players in Vaccine Development

Microorganisms ◽

10.3390/microorganisms9040712 ◽

2021 ◽

Vol 9 (4) ◽

pp. 712

Author(s):

Cristina Cacheiro-Llaguno ◽

Nuria Parody ◽

Marta R. Escutia ◽

Jerónimo Carnés

Keyword(s):

Immune Complexes ◽

Vaccine Development ◽

Correct Diagnosis ◽

Circulating Immune Complexes ◽

Response To Treatment ◽

Canine Leishmaniasis ◽

Leishmania Infection ◽

Serum Samples ◽

Humoral Immune

During canine visceral leishmaniasis (CanL), due to Leishmania infantum (L. infantum), uncontrolled infection leads to a strong humoral immune response. As a consequence of the production of high antibody levels and the prolonged presence of parasite antigens, circulating immune complexes (CIC) are formed, which can be deposited in certain organs and tissues, inducing vasculitis, uveitis, dermatitis and especially glomerulonephritis and renal failure. A method to detect CIC and quantify their levels in serum samples from dogs infected with L. infantum has been recently described. It allowed demonstration of a correlation between CIC levels and disease severity. Thus, CIC measurement may be useful for diagnosis, assessment of disease progression and monitoring response to treatment. This is an interesting finding, considering that there remains an urgent need for identification of novel biomarkers to achieve a correct diagnosis and for optimal disease staging of dogs suffering from Leishmania infection. The objective of the present review is to shed light on the role of CIC in CanL, as well as to highlight their potential use not only as diagnostic and prognostic biomarkers but also as a valuable tool in vaccine development and new immunotherapy strategies to prevent or control disease outcome.

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Circulating Immune Complexes, the Concentration of Serum IgA and the Distribution of HLA Antigens in IgA Nephropathy

Nephron ◽

10.1159/000182350 ◽

1981 ◽

Vol 29 (3-4) ◽

pp. 170-175 ◽

Cited By ~ 24

Author(s):

J. Mustonen ◽

A. Pasternack ◽

H. Helin ◽

A. Rilva ◽

K. Penttinen ◽

...

Keyword(s):

Iga Nephropathy ◽

Immune Complexes ◽

Hla Antigens ◽

Circulating Immune Complexes ◽

Serum Iga

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The in vivo effect of thymic factor (thymostimulin) administration on circulating immune complexes and serum lysozyme levels in untreated Hodgkin's disease patients.

Journal of Clinical Oncology ◽

10.1200/jco.1983.1.2.117 ◽

1983 ◽

Vol 1 (2) ◽

pp. 117-125 ◽

Cited By ~ 4

Author(s):

A Velardi ◽

F Spinozzi ◽

P Rambotti ◽

A Tabilio ◽

A Losito ◽

...

Keyword(s):

Immune Complexes ◽

Hodgkin's Disease ◽

Hodgkin’S Disease ◽

Serum Levels ◽

Mean Value ◽

Circulating Immune Complexes ◽

Calf Thymus ◽

Serum Lysozyme ◽

Thymic Factor

The in vivo effect of a calf thymus extract, thymostimulin, on the levels of circulating immune complexes (CIC) and serum lysozyme was evaluated in 32 patients with untreated Hodgkin's disease. Using the platelet aggregation test for detecting CICs, 12 patients (37%) had positive titers before thymostimulin treatment; 3 patients (10%) remained positive following therapy. Serum levels of Clq-binding immune complexes were evaluated (greater than 24.5 micrograms/ml) in 8 patients prior to thymostimulin therapy (mean value: 42.3 micrograms/ml); 3 patients continued to have elevated levels after treatment. Serum lysozyme levels for Hodgkin's patients was similar to control values (10.6 vs. 8.3 micrograms/ml); however, the Hodgkin's patients with initially elevated CICs had a lower serum lysozyme level than patients with initially normal CICs (12.9 vs. 7.3, p less than 0.02). Thymostimulin increased serum lysozyme levels in the Hodgkin's patients in whom the CICs were initially elevated (7.3 vs. 10.4 micrograms/ml, p less than 0.05). These data suggest that thymostimulin exerts an effect on the nonspecific immune system of Hodgkin's disease patients.

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Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice

Journal of Virology ◽

10.1128/jvi.74.9.4116-4126.2000 ◽

2000 ◽

Vol 74 (9) ◽

pp. 4116-4126 ◽

Cited By ~ 16

Author(s):

Nobutada Tabata ◽

Masaaki Miyazawa ◽

Ryuichi Fujisawa ◽

Yumiko A. Takei ◽

Hiroyuki Abe ◽

...

Keyword(s):

New Zealand ◽

Immune Complexes ◽

Severe Combined Immunodeficiency ◽

Serum Levels ◽

Gene Products ◽

Linkage Studies ◽

Pathogenic Role ◽

Antinuclear Autoantibodies ◽

Glomerular Lesions

ABSTRACT Several strains of mice, including MRL/MpJ mice homozygous for the Fas mutant lpr gene (MRL/lpr mice), F1 hybrids of New Zealand Black and New Zealand White mice, and BXSB/MpJ mice carrying a Y-linked autoimmune acceleration gene, spontaneously develop immune complex-mediated glomerulonephritis. The involvement of the envelope glycoprotein gp70 of an endogenous xenotropic virus in the formation of circulating immune complexes and their deposition in the glomerular lesions have been demonstrated, as has the pathogenicity of various antinuclear, antiphospholipid, and rheumatoid factor autoantibodies. In recent genetic linkage studies as well as in a study of cytokine-induced protection against nephritis development, the strongest association of serum levels of gp70–anti-gp70 immune complexes, rather than the levels of antinuclear autoantibodies, with the development and severity of glomerulonephritis has been demonstrated, suggesting a major pathogenic role of anti-gp70 autoantibodies in the lupus-prone mice. However, the pathogenicity of anti-gp70 autoantibodies has not yet been directly tested. To examine if anti-gp70 autoantibodies induce glomerular pathology, we established from unmanipulated MRL/lpr mice hybridoma clones that secrete monoclonal antibodies reactive with endogenous xenotropic viralenv gene products. Upon transplantation, a high proportion of these anti-gp70 antibody-producing hybridoma clones induced in syngeneic non-autoimmune and severe combined immunodeficiency mice proliferative or wire loop-like glomerular lesions. Furthermore, deposition of gp70 in glomeruli and pathological changes were observed after intravenous injection of representative clones of purified anti-gp70 immunoglobulin G, demonstrating pathogenicity of at least some anti-gp70 autoantibodies.

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Diagnostic Role of Circulating Immune Complexes During Cow Leukemia

Bioscience Biotechnology Research Communications ◽

10.21786/bbrc/13.15/6 ◽

2021 ◽

Vol 13 (15) ◽

pp. 27-29

Author(s):

Talgat R. Yakupov

Keyword(s):

Immune Complexes ◽

Circulating Immune Complexes ◽

Diagnostic Role

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Evaluating the levels of D-dimer as a primary marker in oral cancers using immunoturbidimetric assay: The original research

International Journal of Molecular and Immuno Oncology ◽

10.25259/ijmio_11_2020 ◽

2020 ◽

Vol 5 ◽

pp. 96-100

Author(s):

Ashok K. Vikey ◽

Rajkumar Parwani ◽

Madhusudan Asteker ◽

Deepali Gupta ◽

Simran Parwani

Keyword(s):

Oral Cancer ◽

Cell Biology ◽

Control Group ◽

Original Research ◽

Oral Cancers ◽

Cancer Group ◽

Tissue Factors ◽

Dual Action ◽

And Control ◽

D Dimer

Objectives: Oral cancer is major health threat; with 90% mortality and ranks sixth among worldwide cancers. So to overcome this mortality; newer bio-markers are explored and one of such biomarker is D-dimer, which is end product of fibrinogen formed by plasmin. The raised levels of D-dimer play significant role in proliferation and progress of cancer cells. In cancers D-dimer is formed by dual action, where UPA (Urokinase type Plasminogen Activator) and Tissue factor play important role simultaneously. To understand correlation between D- dimer and oral cancers, by immunoturbidimetry; quantitative assay. Material and Methods: After obtaining consents of patients and Institutional ethical clearance, we randomly selected; age and sex matched; 216 samples. Further these samples were subdivides as oral cancer group and control group, consisting 108 samples in each group respectively. Results: Statistical analysis was done; using SPSS version 20, unpaired -T test, and one way ANOVA were applied. Plasma D-dimer levels were; 497.32±872.28μl/ml and 165.30±150.43 28μl/ml, among cancer and control groups respectively, (P≤ 0.0001), which was statistically highly significant. Conclusion: D-dimer is altered during carcinogenesis by activation of UPA and Tissue factors, and this distinguishes form routine levels of D-dimer. This suggests that, cancer cell biology is greatly affected by D-dimer levels during growth and spread of cancers. So raised levels of D-dimer can be considered during interventions of cancers, and incorporated as a biomarker. However for its scientific applications; there is need of further study, with collaborative approach and larger samples, to restrict cancer related mortalities.

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