Laith AL-Eitan
◽
Fouad Abdelaziz Almomani
◽
Sohaib Mahmoud Al-Khatib
◽
Hanan Abdulraheem Aljamal
◽
Mohammed Nayef Al-Qusami
◽
...
BACKGROUND:
Helicobacter pylori
infection is widespread, affecting about 50% of the global population. Polymorphisms in host genes such as the toll-like receptor 4 (
TLR4
) might affect the susceptibility and severity of infection and treatment success.
OBJECTIVE:
Investigate the susceptibility and severity of
H pylori
infection with host
TLR4
(rs11536889, rs4986790, rs200109652, rs10759932),
TLR5
(rs5744174, rs2072493, rs746250566),
TLR10
(rs559182335, rs10004195) polymorphisms.
DESIGN:
Analytical, cross-sectional.
SETTING:
Endoscopy clinic at tertiary care center.
PATIENTS AND METHODS:
Genomic DNA was extracted from formalin-fixed paraffin-embedded tissues collected from
H pylori
-infected patients and healthy individuals. The single nucleotide polymorphisms (SNPs) within the targeted TLR genes were genotyped to assess the genetic association of various SNPs with disease severity.
MAIN OUTCOME MEASURES:
Effect of genotype distribution on
H pylori
infection.
SAMPLE SIZE:
250 peptic ulcer patients and 217 controls.
RESULTS:
The
TLR10
genotype showed no significant association with
H pylori
infection except for rs10004195 (T>A) (
P
=.002). The genotype frequency of Rs5744174 in
TLR5
had a significant association with the presence of
H pylori
infection (
P
=.046, OR=0.52). Except for gender (P=.022), there were no significant associations between clinical and demographic variables and SNPs relating to the severity of the
H pylori
infections.
CONCLUSIONS:
Our findings are consistent with differences in severity of
H pylori
infection due to TLR SNPs in different ethnic groups. Understanding differences in genetic susceptibility could help in classifying patients and matching patients with various treatment options on a genetic basis.
LIMITATIONS:
Lack of
H pylori
pathogenicity features assessment.
CONFLICTS OF INTEREST:
None.
Lack of association between gene polymorphisms of Angiotensin converting enzyme, Nod-like receptor 1, Toll-like receptor 4, FAS/FASL and the presence of Helicobacter pylori-induced premalignant gastric lesions and gastric cancer in Caucasians
