Experimental evidence of collective vibrations in DNA double helix (Raman spectroscopy)

1983 ◽  
Vol 78 (10) ◽  
pp. 5937-5939 ◽  
Author(s):  
Hisako Urabe ◽  
Yasunori Tominaga ◽  
Kenji Kubota
Keyword(s):  
Raman Spectroscopy ◽  
Experimental Evidence ◽  
Double Helix ◽  
Collective Vibrations ◽  
Dna Double Helix

Coupled dynamics between DNA double helix and hydrated water by low frequency Raman spectroscopy

1985 ◽  
Vol 83 (11) ◽  
pp. 5972-5975 ◽  
Author(s):  
Yasunori Tominaga ◽  
Megumi Shida ◽  
Kenji Kubota ◽  
Hisako Urabe ◽  
Yoshifumi Nishimura ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Double Helix ◽  
Low Frequency ◽  
Coupled Dynamics ◽  
Dna Double Helix

Low-lying collective modes of DNA double helix by Raman spectroscopy

Biopolymers ◽  
1982 ◽  
Vol 21 (12) ◽  
pp. 2477-2481 ◽  
Author(s):  
Hisako Urabe ◽  
Yasunori Tominaga
Keyword(s):  
Raman Spectroscopy ◽  
Double Helix ◽  
Collective Modes ◽  
Dna Double Helix

Protamine-DNA interaction

1978 ◽  
Vol 36 (2) ◽  
pp. 200-201
Author(s):  
D.P. Bazett-Jones ◽  
F.P. Ottensmeyer
Keyword(s):  
Small Volume ◽  
Double Helix ◽  
Dna Interaction ◽  
Dark Field ◽  
Sperm Head ◽  
Nuclear Proteins ◽  
Field Electron Microscopy ◽  
Dark Field Electron ◽  
Dna Double Helix ◽  
Positively Charged

Dark field electron microscopy has been used for the study of the structure of individual macromolecules with a resolution to at least the 5Å level. The use of this technique has been extended to the investigation of structure of interacting molecules, particularly the interaction between DNA and fish protamine, a class of basic nuclear proteins of molecular weight 4,000 daltons.Protamine, which is synthesized during spermatogenesis, binds to chromatin, displaces the somatic histones and wraps up the DNA to fit into the small volume of the sperm head. It has been proposed that protamine, existing as an extended polypeptide, winds around the minor groove of the DNA double helix, with protamine's positively-charged arginines lining up with the negatively-charged phosphates of DNA. However, viewing protamine as an extended protein is inconsistent with the results obtained in our laboratory.

Mercury Electrodes in Nucleic Acid Electrochemistry: Sensitive Analytical Tools and Probes of DNA Structure. A Review

2004 ◽  
Vol 69 (4) ◽  
pp. 715-747 ◽  
Author(s):  
Miroslav Fojta
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Double Helix ◽  
Dna Structure ◽  
Electrochemical Analysis ◽  
Label Free ◽  
Helix Formation ◽  
Mercury Electrodes ◽  
Dna Strand ◽  
Dna Double Helix

This review is devoted to applications of mercury electrodes in the electrochemical analysis of nucleic acids and in studies of DNA structure and interactions. At the mercury electrodes, nucleic acids yield faradaic signals due to redox processes involving adenine, cytosine and guanine residues, and tensammetric signals due to adsorption/desorption of polynucleotide chains at the electrode surface. Some of these signals are highly sensitive to DNA structure, providing information about conformation changes of the DNA double helix, formation of DNA strand breaks as well as covalent or non-covalent DNA interactions with small molecules (including genotoxic agents, drugs, etc.). Measurements at mercury electrodes allow for determination of small quantities of unmodified or electrochemically labeled nucleic acids. DNA-modified mercury electrodes have been used as biodetectors for DNA damaging agents or as detection electrodes in DNA hybridization assays. Mercury film and solid amalgam electrodes possess similar features in the nucleic acid analysis to mercury drop electrodes. On the contrary, intrinsic (label-free) DNA electrochemical responses at other (non-mercury) solid electrodes cannot provide information about small changes of the DNA structure. A review with 188 references.

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