scholarly journals The effect of mung bean sprouts (Phaseolus radiatus L.) to lipid profile of male Sprague-Dawley rats fed with high-fat diet

2016 ◽  
Author(s):  
Muhammad Asrullah ◽  
Lily Arsanti Lestari ◽  
Siti Helmyati ◽  
Arta Farmawati
Keyword(s):  
Lipid Profile ◽  
High Fat Diet ◽  
Mung Bean ◽  
Sprague Dawley ◽  
High Fat ◽  
Mung Bean Sprouts ◽  
Sprague Dawley Rats ◽  
Bean Sprouts

scholarly journals Pengaruh pemberian kecambah kacang hijau (Phaseolus radiatus (L.)) terhadap kadar malondealdehid (MDA) plasma dan jaringan hati tikus Sprague Dawley yang diberi pakan lemak tinggi

2016 ◽  
Vol 13 (2) ◽  
pp. 82
Author(s):  
Novidiyanto Novidiyanto ◽  
Arta Farmawati ◽  
Lily Arsanti Lestari
Keyword(s):  
Lipid Peroxidation ◽  
Plasma Level ◽  
High Fat Diet ◽  
Mung Bean ◽  
Liver Tissue ◽  
Control Group ◽  
Sprague Dawley ◽  
High Fat ◽  
Mung Bean Sprouts ◽  
Bean Sprouts

Background: High-fat diet increases the levels of fat, especially cholesterol and triglycerides that cause hyperlipidemia. Polyunsaturated fatty acids are very easily oxidized by free radicals reactive oxygens species (ROS) called lipid peroxidation, to produce compounds that malondialdehyde (MDA), is toxic and can damage plasma membrane and liver tissue. Mung bean sprouts are known to contain antioxidants which act to inhibit lipid peroxidation process.Objective: Determine the effect of mung bean sprouts to the level of plasma MDA and liver tissue of rats with high-fat diets.Method: This research is an experimental study with an only post-test design with the control group. Thirty-five male Sprague-Dawley rats were divided into 5 groups. Group K1 received fed standard; K2 received high-fat diet without mung bean sprouts. Group P1 received high-fat diet and mung bean sprouts dose of  0.5 mL/gBW. Group P2 received high-fat diet and mung bean sprouts dose of 1 mL/gBW. Group P3 received high-fat diet and vitamin E doses of 23 IU. All group were treated for 28 days. Analysis of MDA plasma and liver tissue performed after treatment.Results: Group K1 has plasma MDA levels of 1.17 ± 0.14 nmol/mL Mean MDA plasma level in the group K1 Group of K1 Mean MDA plasma level in the group K2 (39.7±0.19 nmol/mL) was higher than the group of K1 (1,17±0,14 nmol/mL), group P1 (2.94±0.09 nmol/mL), group P2 (1.73±0.08 nmol/mL) and group P3 (1.53±0.07 nmol/mL). Group K1 has liver tissue MDA Levels of 1,64±0,11 nmol/g. Mean MDA liver tissue level in the group of K2 (4.90±0.29 nmol/g) was higher than the group of P1 (3.68±0.45 nmol/g), group P2 (2.27±0.10 nmol/g), and group P3 (2.01±0.11 nmol/g).Conclusion: Level of MDA plasma and liver tissue of rat in group P1 and P2 was lower than a level of MDA plasma and liver tissue of rat in group K1, but higher than the level of MDA plasma and liver tissue of rat in group P3. There was a very strong correlation between the level of MDA plasma and liver tissue of rat was given the mung bean sprouts and high-fat diet.

scholarly journals Pencegahan hipertensi dan penebalan dinding aorta dengan pemberian kecambah kacang hijau (Phaseolus radiatus (L)) pada tikus putih Sprague Dawley

2016 ◽  
Vol 12 (3) ◽  
pp. 116
Author(s):  
Novian Swasono Hadi ◽  
Arta Farmawati ◽  
Ahmad Ghozali
Keyword(s):  
Blood Pressure ◽  
Vitamin E ◽  
High Fat Diet ◽  
Mung Bean ◽  
Male Rats ◽  
Sprague Dawley ◽  
High Fat ◽  
Bean Sprout ◽  
Mung Bean Sprouts ◽  
Bean Sprouts

Background: Lifestyle changes with high-fat food consumption is one of the factors the risks of cardiovascular diseases like of coronary heart disease and atherosclerosis. A healthy diet and a balanced diet and consume foods that contain lots of antioxidants is one of the effective ways to prevent hyperlipidemia. Mung bean sprouts have properties that neutralize free radicals cause Hyperlipidemia and cardiovascular diseases because it is an antioxidant compound.Objective: The aim of this study was to determinate the effect of mung bean sprouts (Phaseolus radiatus (L)) to blood pressure and histopathology aorta of Sprague-Dawley male rats.Method: The type of study was experimental research using pre-post test controlled group design for blood pressure variable and post test only controlled group design histopathology aorta. The thirty-five of Sprague-Dawley male rats was eight weeks divided into 5 groups. The first group was given standard diet, group 2 was given a hight fat diet, the third group was given a high-fat diet and mung bean sprout 0,67 gram, group 4 was given a high-fat diet and mung bean 1,34 gram, and group 5 was given a high-fat diet and vitamin E doses of 23 IU.Results: Result of this study showed that after 4 weeks of treatment, increased in blood pressure systole in the given of  high fat diet higher than group who were given a high fat diet and mung bean sprout and also on group who were given high fat diet and vitamin E, but there is no difference effect a decrease in blood pressure between the provision of mung bean sprouts and vitamin E (p>0,05). Statistical analysis to thick the wall the aorta show the similarity meaningful in all the treatment group, it can be said that overall thick the wall the aorta in this research is not different.Conclusion: A dose of mung bean sprout 0,67 g is optimal doses in preventing a rise in blood pressure and prevent alterations histopathology Sprague-Dawley male rats.

scholarly journals Effects ofPiper nigrumextracts: Restorative perspectives of high-fat diet-induced changes on lipid profile, body composition, and hormones in Sprague–Dawley rats

2015 ◽  
Vol 53 (9) ◽  
pp. 1318-1328 ◽  
Author(s):  
BrahmaNaidu Parim ◽  
Nemani Harishankar ◽  
Meriga Balaji ◽  
Sailaja Pothana ◽  
Ramgopal Rao Sajjalaguddam
Keyword(s):  
Body Composition ◽  
Lipid Profile ◽  
High Fat Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Induced Changes

scholarly journals Peran kecambah kacang hijau (Phaseolus radiatus (L.)) pada sel lemak retroperitoneal tikus Sprague Dawley yang diberi diet tinggi lemak

2019 ◽  
Vol 16 (1) ◽  
pp. 31
Author(s):  
Dwi Lestari ◽  
Wiryatun Lestariana ◽  
Lily Arsanti Lestari ◽  
Arta Farmawati
Keyword(s):  
Vitamin E ◽  
Mung Bean ◽  
Feeding Tube ◽  
Cell Number ◽  
Cell Diameter ◽  
Sprague Dawley ◽  
High Fat ◽  
Bean Sprout ◽  
Mung Bean Sprouts ◽  
Bean Sprouts

The role of mung bean sprouts (Phaseolus radiatus (L.)) on retroperitoneal fat cells of Sprague Dawley rats fed a high-fat dietBackground: The tendency of current lifestyles with high consumption patterns of fat is one of the factors causing obesity which is a risk of cardiovascular disease. Prevention of cardiovascular disease can be done by adopting a dietary pattern that is rich in antioxidants including consumption of sources of vitamin E. Sprouts from mung beans contain vitamin E and phytochemicals rich in antioxidants, so they can be used as an alternative to prevent hyperlipidemia sourced from daily functional food.Objective: To assess the effects of mung bean sprouts on retroperitoneal fat weight, cell number and cell diameter in rats fed high-fat feed.Method: This research is an experimental study with a post-test design with the control group. Thirty-four male Sprague Dawley rats ± 2 months old were divided into five groups, group I (standard feed), group II (high-fat feed), group III (high-fat feed and intervention of feeding tube of mung bean sprout 0.67 g/ 200 g BW), group IV (high fat feed and intervention of feeding tube of mung bean sprout 1.34 g / 200 g BW), and group V (high-fat feed and 23 IU dose of vitamin E supplement with feeding tube). After adaptation for three days, the intervention was carried out for four weeks. Analysis of fat weight, fat cell number, and fat cell diameter were done after the treatments. Data analysis using the One-Way ANOVA test. Results: Mung bean sprout dose of 1.34 g given for 4 weeks did not significantly decrease fat weight and cell number, but it decreased the diameter of retroperitoneal fat cells.Conclusion: Mung bean sprouts dose 1.34 g was better than a dose of 0.67 g and vitamin E supplementation in reducing the diameter of retroperitoneal fat cells in rats fed a high-fat diet.

scholarly journals Effect Supplementation of Mung Bean Sprouts (Phaseolus radiatus L.) and Vitamin E in Rats Fed High Fat Diet

2019 ◽  
Vol 4 (11) ◽  
pp. 36
Author(s):  
Novidiyanto . ◽  
M Asrullah ◽  
L Arsanti Lestari ◽  
S Helmyati ◽  
A Farmawati
Keyword(s):  
Vitamin E ◽  
High Fat Diet ◽  
Mung Bean ◽  
High Fat ◽  
Mung Bean Sprouts ◽  
Bean Sprouts

.

The Effects of Maternal High Fat Diet on the Lingual CD36 Lipid Sensors of the Offspring Sprague Dawley Rats

Metabolism ◽  
2021 ◽  
Vol 116 ◽  
pp. 154497
Author(s):  
Elif Günalan ◽  
Meyli Ezgi Karagöz ◽  
Bayram Yılmaz ◽  
Burcu Gemici
Keyword(s):  
High Fat Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Lipid Sensors

Abstract 382: Anti-MAA Antibodies in Sprague Dawley Rats are Increased in Response to a High Fat Western Diet

2013 ◽  
Vol 33 (suppl_1) ◽  
Author(s):  
Michael J Duryee ◽  
Anand Dusad ◽  
Scott W Shurmur ◽  
Michael D Johnston ◽  
Robert P Garvin ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Normal Diet ◽  
Western Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Stable Plaque ◽  
Circulating Antibodies ◽  
Modified Proteins ◽  
Progression Of Atherosclerosis

Introduction Malondialdehyde/Acetaldehyde (MAA) modified proteins have been suggested to play a role in the development/progression of atherosclerosis. Circulating antibodies directed against these proteins have recently been shown to be associated with the severity of the disease. More specifically, the isotype of the antibody to MAA correlated with either an acute MI (IgG) or stable plaque formation (IgA) formation. MAA is thought to form as a result of the oxidation of fat(s) and thus the concentration and antibody response should reflect the amount of fat in the diet. Objective The purpose of this study was to evaluate the antibody responses to MAA modified proteins following immunization and high fat western diet feeding in rats. Methods Male Sprague Dawley rats were immunized with MAA-modified protein weekly for 5 weeks and then assayed for antibodies to these proteins. Animals were then separated into the following groups: chow sham, chow MAA immunized, high fat sham, and high fat MAA immunized. The high fat animals were fed a Western diet with 2-thiouracil for 12 weeks, bled every 3 weeks, and serum assayed for the presence of circulating MAA antibodies. Results Prior to feeding with high fat diet, rats immunized with MAA-modified protein had a significant increase (P<0.001) in serum antibodies directed against these modified proteins compared to controls (N of 4 per group). Following feeding of high fat diet antibody concentrations increased 6 fold in the high fat MAA immunized group compared to the chow MAA immunized group (P<0.05). Antibodies in the high fat sham and chow sham had only minimal increases in antibodies to these proteins. Conclusions These data demonstrate that following immunization with MAA-modified proteins, circulating antibodies are produced that increase following consumption of a high fat Western diet. It suggests that MAA-modified proteins are produced at low levels following normal diet, producing antibodies which act as a normal clearance method for altered protein. When high fat consumption increases these antibody levels are increased in response to the oxidative stress. Implications Use of these antibodies as a biomarker in the future may help predict the onset or progression of atherosclerosis.

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