scholarly journals The profile of ovarian tissue of female Sprague-Dawley rats (Rattus norvegicus L.) after vitrification using ethylene glycol and egg yolk as cryoprotectant

2019 ◽  
Author(s):  
A. Awanis ◽  
N. Anita ◽  
A. Abinawanto ◽  
A. A. Jusuf ◽  
I. Muhiardi ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Rattus Norvegicus ◽  
Ovarian Tissue ◽  
Egg Yolk ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Pengaruh Pemberian Kurma Muda (Phoenix dactylifera) Terhadap Kadar FSH dan Reseptor FSH Ovarium Tikus Putih (Rattus norvegicus)

2019 ◽  
Vol 7 (1) ◽  
pp. 117-121
Author(s):  
Herlambang Herlambang ◽  
Ave Olivia Rahman ◽  
Erny Kusdiyah
Keyword(s):  
Ovarian Tissue ◽  
Scientific Evidence ◽  
Phoenix Dactylifera ◽  
Group Iv ◽  
Sprague Dawley ◽  
Female Reproduction ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Vaginal Swabs ◽  
The Mean

ABSTRACT Background: Infertility may give an impact on psychosocial. In Indonesia, the habit of consuming young dates is often done by couples to increase fertility. FSH is one of the factors that play a role in folliculogenesis. The lack of scientific evidence of young dates consumption effects on female reproduction has led to this study. This study aims to determine the levels of FSH hormone and the picture of ovarian tissue in mice after administration of young dates. Method: This study used an experimental design using 28 female Sprague-Dawley rats which were randomly divided into 4 groups. Group I to III was given young dates in successive doses: 17 mg, 34 mg, 68 mg per 200 grams of body weight and group IV was given distilled water. The treatment was carried out for 28 days. FSH levels were examined before treatment during the proestrus phase which was known from microscopic examination of rat vaginal swabs. The treatment begins during the proestrus phase. Result: The mean baseline and post-treatment FSH levels were 0.08 and 0.09 respectively Conclusion: There is no significant increase of FSH levels and FSH receptors as the effect of giving young dates (Phoenix Dactylifera) to female Sprague-Dawley rats Keywords: Dates, FSH, FSH receptors, Rats   ABSTRAK Latar Belakang: Infertilitas dapat memberikan dampak psikososial. Di Indonesia, kebiasaan mengkonsumsi kurma muda sering dilakukan oleh pasangan untuk meningkatkan kesuburan. Hormon FSH merupakan salah satu faktor yang berperan dalam folikelgenesis.  Belum adanya bukti ilmiah efek konsumsi buah kurma muda terhadap reproduksi wanita mendorong dilakukannya studi ini. Studi ini bertujuan mengetahui kadar hormon FSH dan gambaran jaringan ovarium pada tikus setelah pemberian kurma muda. Metode: Penelitian ini menggunakan desain ekperimental menggunakan tikus Sprague dawney betina sebanyak 28 ekor yang dibagi secara acak menjadi 4 kelompok. Kelompok I-III diberikan kurma muda dosis berturut -turut 17 mg, 34 mg, 68 mg per 200 gram BB dan kelompok IV diberikan aquadest. Perlakuan dilakukan selama 28 hari. Kadar hormon FSH diperiksa sebelum perlakuan saat fase proestrus yang diketahui dari pemeriksaan mikroskopis swab vagina tikus. Perlakuan dimulai saat fase proestrus. Hasil: Rerata kadar FSH baseline dan paska perlakuan adalah 0,08 dan 0,09 Kesimpulan: Tidak terdapat peningkatan yang signifikan kadar FSH dan reseptor FSH terhadap efek pemberian buah kurma muda (Phoenix Dactylifera) pada uterus tikus Spague Dawney Kata kunci : kurma, FSH, Reseptor FSH, tikus

scholarly journals Aversion to Desflurane and Isoflurane in Sprague-Dawley Rats (Rattus norvegicus)

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 950 ◽  
Author(s):  
Katrina Frost ◽  
Maaria Shah ◽  
Vivian S.Y. Leung ◽  
Daniel S.J. Pang
Keyword(s):  
Carbon Dioxide ◽  
Exposure Time ◽  
Washout Period ◽  
Rattus Norvegicus ◽  
Sprague Dawley ◽  
Initial Exposure ◽  
Treatment Groups ◽  
Avoidance Paradigm ◽  
Sprague Dawley Rats

Carbon dioxide and isoflurane are widely used for killing rats, yet may not truly achieve “euthanasia”, because they elicit aversion. The inhalant anesthetic desflurane is faster acting than isoflurane, representing a potential refinement. Using an aversion-avoidance paradigm, 24 rats were exposed to isoflurane or desflurane (n = 12 per group) at initial exposure. Fourteen rats were then re-exposed to isoflurane or desflurane (n = 7 per group), after a 7 days washout period. Initial exposure: time to recumbency was faster for desflurane than isoflurane (p = 0.0008, 95% CI [-12.9 to 32.6 s]), with 9/12 and 6/12 rats becoming recumbent, respectively. At initial exposure, there was no difference between groups in time to withdrawal (p = 0.714). At re-exposure, all rats withdrew and no rats became recumbent. Time to withdrawal at re-exposure did not differ between treatment groups (p = 0.083). Compared to initial exposure, time to withdrawal during re-exposure was similar for isoflurane (p = 0.228) and faster with desflurane (p = 0.012, 95% CI [19.1 to 49.5 s]). Isoflurane and desflurane are similarly aversive, with aversion increasing at re-exposure. The shorter time from exposure to recumbency with desflurane indicates that any distress is of a shorter duration when compared with isoflurane.

scholarly journals Intraperitoneal Alfaxalone and Alfaxalone–Dexmedetomidine Anesthesia in Sprague–Dawley Rats (Rattus norvegicus)

2020 ◽  
Vol 59 (5) ◽  
pp. 531-538
Author(s):  
Sylvia E West ◽  
Jonathan C Lee ◽  
Tinika N Johns ◽  
Elizabeth A Nunamaker
Keyword(s):  
Rattus Norvegicus ◽  
Female Rats ◽  
Male Rats ◽  
Sprague Dawley ◽  
Male And Female ◽  
Withdrawal Reflex ◽  
Male And Female Rats ◽  
Sprague Dawley Rats ◽  
Laboratory Rodent ◽  
Physiologic Parameters

Due to their unpredictability and variable effects, injectable anesthetic regimens in laboratory rodent species warrant refinement. In our study we sought to evaluate alfaxalone, which has gained recent popularity in veterinary medicine, alone and in combination with dexmedetomidine to evaluate their anesthetic ability in Sprague–Dawley rats when administered intraperitoneally. Three doses of alfaxalone only and 4 dose combinations of alfaxalone-dexmedetomidine were tested in males and female rats. The time to induction, anesthetic duration, pulse rate, respiratory rate, temperature, and time to recovery were recorded by a blind observer. The level of anesthesia induced by the various anesthetic protocols was assessed by using pedal withdrawal reflex to a noxious stimulus and scored according to the response. Dependent on the treatment group, atipamezole or saline was administered intraperitoneally once animals reached 60 min of anesthesia. Regardless of the dose, alfaxalone alone achieved only a sedative level of anesthesia, whereas all alfaxalone-dexmedetomidine combinations led to a surgical level of anesthesia in all animals. Anesthesia regimens using alfaxalone alone and in combination with dexmedetomidine demonstrated sex-associated differences, with female rats maintaining longer durations of sedation or anesthesia than their male counterparts. Both male and female rats displayed decreases in physiologic parameters consistent with the effects of dexmedetomidine. Given the results described herein, we recommend 20 mg/kg alfaxalone for sedation and 30 mg/kg alfaxalone combined with 0.05 mg/kg dexmedetomidine for surgical anesthesia in female rats. Appropriate doses of alfaxalone only and alfaxalone-dexmedetomidine for male rats were not determined in this study and need further evaluation.

Antilithiatic activity of a non-pharmacopoeial Unani formulation in chemically induced urolithiasis in rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Rehana Parveen ◽  
Abdul Wadud ◽  
Shariq Shamsi ◽  
Shaista Parveen
Keyword(s):  
Ethylene Glycol ◽  
Urine Volume ◽  
Urinary Calcium ◽  
Urinary Stones ◽  
Test Drug ◽  
Sprague Dawley ◽  
Hydroalcoholic Extract ◽  
Sprague Dawley Rats ◽  
Chemically Induced ◽  
Control Disease

Abstract Objectives Parshioshan (Adiantum capillus-veneris L.), Duqu (Peucedanum grande C.B. Clarke), Kaknaj (Physalis alkekengi L.) and Kharekhasak (Tribulus terresteris L.) have been selected for this study as they have been associated with medicinal actions for litholytic activity. Methods The experiment was carried out in Sprague Dawley rats divided into seven groups, serving as plain control, disease control, standard control, curative A and B and preventive A and B groups. Animals of plain control received distilled water. Remaining six groups received Ethylene glycol 0.75% and Ammonium chloride 1% by adding in the drinking water for the first three days followed by 0.75% Ethylene glycol for 18 days. From 8th day till 21st day, standard control received Cystone in the dose of 750 mg/kg. Preventive and curative test groups were treated with hydroalcoholic extract of the test drug in the dose of 132 mg/kg and 264 mg/kg from 1st to 21st day and 8th to 21st day of calculi induction. Results Test drug reduced the number of calcium oxalate crystals in the urine; the level of urinary calcium, creatinine, magnesium, phosphorus, sodium and chloride decreased significantly in standard and test groups. The urine volume increased significantly in all the test groups. The level of serum calcium, urea, phosphorus and creatinine were significantly reduced in all the test groups. Conclusions These results indicated that the test drug reduced and prevented the growth of urinary stones. Moreover, the test drug also possessed significant antiurolithiatic activity. However, the protective effect was found more than its curative effect.

scholarly journals Immunodetection of Osteopontin at Sites of Resorption in the Pulp of Rat Molars

2002 ◽  
Vol 50 (7) ◽  
pp. 911-921 ◽  
Author(s):  
Yoshihito Shimazu ◽  
Antonio Nanci ◽  
Takaaki Aoba
Keyword(s):  
Egg Yolk ◽  
Tartrate Resistant Acid Phosphatase ◽  
Sprague Dawley ◽  
Resorption Lacuna ◽  
Sprague Dawley Rats ◽  
Golgi Region ◽  
Cytoplasmic Vesicles ◽  
Mediate Cell ◽  
Chicken Egg Yolk ◽  
Rat Molars

Osteopontin (OPN) has been proposed to act as a substrate for osteoclast adhesion during bone resorption. The aim of the present study was to examine the presence and distribution of OPN at sites of resorption in traumatized radicular pulp. The upper first molars of 6-week-old male Sprague-Dawley rats were luxated and then repositioned in the original sockets. The animals were sacrificed by intracardiac perfusion at 10 and 14 days after tooth reimplantation. The teeth were decalcified in EDTA and then processed for embedding in paraffin for histochemistry or LR White resin for immunocytochemistry. Odontoclasts were identified by their multinucleated morphology and expression of tartrate-resistant acid phosphatase (TRAP). Osteopontin was immunolocalized using postem-bedding colloidal gold labeling with a chicken egg yolk anti-rat OPN antibody. After reimplantation of the teeth, TRAP-positive cells were present along the pulp dentin wall. Osteopontin was not consistently detected at exposed predentin/dentin surfaces. However, gold particles were often found at the margin of resorption lacunae. Labeling was also seen over the Golgi region and cytoplasmic vesicles of odontoclasts and of neutrophils and fibroblast-like cells. The results suggest that accumulation of OPN at the predentin/dentin surface is not a prerequisite for adhesion of odontoclasts to the wall substance and that recruited odontoclasts produce OPN locally to mediate cell and/or matrix events within the resorption lacuna.

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