Interspecific amplification of tea tree (Melaleuca alternifolia-Myrtaceae) microsatellite loci-potential implications for conservation studies

2000 ◽  
Vol 48 (3) ◽  
pp. 367 ◽  
Author(s):  
M. Rossetto ◽  
F. C. L. Harriss ◽  
A. Mclauchlan ◽  
R. J. Henry ◽  
P. R. Baverstock ◽  
...  

This study investigated the interspecific amplification of 35 microsatellite loci developed for M. alternifolia across seven other species within the Myrtaceae. All the primers used gave successful amplification of loci in at least one of the species tested. The level of success varied between species; 88.6% of primers gave amplification products for Melaleuca spp., 74.3% for Callistemon salignus, 45.7% for Eucalyptus spp. and 25.7% for Backhousia citriodora. Sequencing of a number of amplification products confirmed the presence of microsatellites in those loci. This study shows that the development of species-specific microsatellite libraries might not always be necessary. Cross-species amplification could enable the application of microsatellite technology to studies with limited resources, a feature characteristic of conservation projects.

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257031
Author(s):  
Francesco Martoni ◽  
Mark J. Blacket

Psyllids, also known as jumping plant lice, are phloem feeding Hemiptera that often show a strict species-specific relationship with their host plants. When psyllid-plant associations involve economically important crops, this may lead to the recognition of a psyllid species as an agricultural or horticultural pest. The Australian endemic tea tree, Melaleuca alternifolia (Maiden & Betche) Cheel., has been used for more than a century to extract essential oils and, long before that, as a traditional medicine by Indigenous Australian people. Recently, a triozid species has been found to damage the new growth of tea trees both in Queensland and New South Wales, raising interest around this previously undocumented pest. Furthermore, adults of the same species were also collected from Citrus plantations, leading to potential false-positive records of the exotic pest Trioza erytreae (Del Guercio 1918), the African Citrus psyllid. Here we describe for the first time Trioza melaleucae Martoni sp. nov. providing information on its distribution, host plant associations and phylogenetic relationships to other Trioza species. This work enables both morphological and molecular identification of this new species, allowing it to be recognized and distinguished for the first time from exotic pests as well as other Australian native psyllids. Furthermore, the haplotype network analysis presented here suggests a close relationship between Trioza melaleucae and the other Myrtaceae-feeding Trioza spp. from Australia, New Zealand, and Taiwan.


Aquaculture ◽  
2021 ◽  
pp. 736954
Author(s):  
Tamires R. dos Reis ◽  
Matheus D. Baldissera ◽  
Carine F. Souza ◽  
Bernardo Baldisserotto ◽  
Julia Corá Segat ◽  
...  

2010 ◽  
Vol 58 (5) ◽  
pp. 373 ◽  
Author(s):  
L. Baskorowati ◽  
M. W. Moncur ◽  
J. C. Doran ◽  
P. J. Kanowski

Melaleuca alternifolia (Maiden & Betche) Cheel is commercially important as the source of essential oil for the Australian tea tree-oil industry. Information on reproductive biology of M. alternifolia is important to the Australian breeding program directed at improving the quality and quantity of tea tree oil. Flowering in three geographically separated sites – two planted seed orchards and one managed natural population, all in NSW – was observed in the present study, with supporting data obtained from glasshouse-grown plants in Canberra. The majority of the work was conducted from 2004 to 2007, although the study also drew on some prior observations. M. alternifolia has spikes of flowers that open acropetally over a 6-day period. No strong separation of male and female phases was found in any individual flower; pollen was shed by 1.4 days after anthesis and the stigma reached peak receptivity 3–5 days after anthesis. Dichogamy and acropetal floral development may lead to geitonogamy. Flowering occurred during the months of October–November, with the peak in November, and was synchronous across all three sites. Flowering intensity and success in producing capsules appeared to be associated with total spring rainfall. Initiation of flowering in M. alternifolia appears to be correlated with daylength, or an environmental parameter closely correlated with daylength. Flowering intensity varied considerably among the years surveyed, sites and families, and appears to be promoted by a period of winter minimum temperatures below 5°C. In M. alternifolia, the morphological development of buds, flowers and fruit leading to the development of mature seed takes place over a period 16–18 months from flowering. M. alternifolia differed significantly in the number of viable seeds per capsule from individual trees, from 26 ± 3.8 to 57 ± 3.8 germinants.


2011 ◽  
Vol 13 (4) ◽  
pp. 492-499 ◽  
Author(s):  
A.C.M. Oliveira ◽  
A. Fontana ◽  
T.C. Negrini ◽  
M.N.M. Nogueira ◽  
T.B.L. Bedran ◽  
...  

O interesse por medicamentos alternativos, principalmente daqueles provenientes de extratos naturais, tem aumentado nas últimas décadas. A Melaleuca alternifolia é um arbusto pertencente ao gênero Melaleuca, popularmente conhecida como "árvore de chá", cujo principal produto é o óleo essencial (TTO - tea tree oil), de grande importância medicinal por possuir comprovada ação bactericida e antifúngica contra diversos patógenos humanos. Em virtude da atividade terapêutica em diversas especialidades médicas, o TTO passou a ser empregado na área odontológica. Esta revisão de literatura foi realizada com o objetivo de discutir os ensaios já realizados com o TTO contra microrganismos relacionados à doença cárie, doença periodontal e problemas pulpares. O óleo de Melaleuca tem demonstrado boa ação antibacteriana in vitro contra microrganismos bucais, porém, pesquisas envolvendo o estudo do mecanismo de ação sobre as células microbianas ou estudos in vivo ainda são escassos e precisam ser realizados, já que esse produto pode ser útil na odontologia, seja na manutenção química da higiene ou prevenção de doenças bucais.


2008 ◽  
Vol 18 (12) ◽  
pp. 1644-1650 ◽  
Author(s):  
Seun-Ah Yang ◽  
Sang-Kyung Jeon ◽  
Eun-Jung Lee ◽  
Nam-Kyung Im ◽  
Ji-Young Jung ◽  
...  

2021 ◽  
Author(s):  
Alpna Bisht ◽  
Chetna Hemrajani ◽  
Navneet Upadhyay ◽  
Prakriti Nidhi ◽  
Rajan Rolta ◽  
...  

Aim: Azelaic acid (AzA), a comedolytic, antibacterial, anti-inflammatory anti-melanogenic agent, prescribed against acne vulgaris is safe on skin. Its combination with another widely used anti-acne agent, tea tree oil (EO) whose delivery is limited by volatility, instability and lipophilicity constraints was attempted. Method: Solvent injection was used to prepare AzA-EO integrated ethosomes. Result: Ethosomes were transformed into carbopol hydrogel, which exhibited pseudo-plastic properties with appreciable firmness, work of shear, stickiness and work of adhesion. The hydrogel showed better permeation and retention characteristics vis-a-vis commercial formulation (AzidermTM), when evaluated in Wistar rat skin. Further, ethosome hydrogel composite was better tolerated with no side effects. Conclusion: The findings suggests that the aforementioned strategy could be a potential treatment used for acne management.


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