Combining ability and heterosis analysis for mineral elements by using cytoplasmic male-sterile systems in non-heading Chinese cabbage (Brassica rapa)

2018 ◽  
Vol 69 (3) ◽  
pp. 296 ◽  
Author(s):  
Fei Xie ◽  
Jun Zha ◽  
Hongyu Tang ◽  
Yuying Xu ◽  
Xujia Liu ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Combining Ability ◽  
Nutritional Quality ◽  
Mineral Elements ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Positive Effects ◽  
Cytoplasmic Effects ◽  
Cms Line ◽  
Heading Chinese Cabbage

Brassica vegetables are an important source of dietary nutrition. The nutritional quality of mineral elements is becoming one of the most important studied traits because of the year-round supply of vegetables in China. However, there are few reports about breeding and utilisation of mineral elements in non-heading Chinese cabbage (Brassica napus L.). Using two newly reported CMS (cytoplasmic male-sterile) lines of non-heading Chinese cabbage, we conducted incomplete diallel experiments to analyse heterosis, combining ability and cytoplasmic effects for mineral elements such as calcium (Ca), iron (Fe), magnesium (Mg) and zinc (Zn). Heterosis analysis of mineral elements indicated that the crossing combinations A1 (hau CMS) × C03, A2 (eru CMS) × C03 and A2 × C11 exhibited desirable positive effects of mid-parent heterosis and high-parent heterosis in terms of mineral element content that could be exploited for commercial purposes. Analysis of general combining ability (GCA) effects of the parental lines indicated that the tester C11 was superior for the improvement of the four mineral elements; CMS line A1 had greater GCA effects than CMS line A2 for Ca and Fe. The hybrid combinations A2 × C11, B × C05 and B × C12 showed positive specific combining ability (SCA) effects for the four mineral elements on overall performance. The analysis revealed that cytoplasmic effects of hau CMS and eru CMS were both positive for Ca and Fe, and that A1 had more obvious cytoplasmic effects than did A2. These results indicated that the two isonuclear, alloplasmic CMS lines of non-heading Chinese cabbage might be useful for improving the nutritional quality traits of cruciferous vegetables and for heterosis utilisation.

Microtubule and male sterility in a gene-cytoplasmic male sterile line of non-heading Chinese cabbage

2012 ◽  
Vol 92 (15) ◽  
pp. 3046-3054 ◽  
Author(s):  
Jingyi Zhang ◽  
Changwei Zhang ◽  
Yan Cheng ◽  
Li Qi ◽  
Shumin Wang ◽  
...  
Keyword(s):  
Male Sterility ◽  
Chinese Cabbage ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Cytoplasmic Male Sterile ◽  
Heading Chinese Cabbage

scholarly journals Changes of Endogenous Hormones and Polyamines in Cytoplasmic Male Sterile Non-heading Chinese Cabbage During Anther Development

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 881D-881
Author(s):  
Gong-Jun Shi* ◽  
Xi-Lin Hou ◽  
Wei Hu ◽  
Zhong-Chun Jiang
Keyword(s):  
Chinese Cabbage ◽  
Brassica Campestris ◽  
Anther Development ◽  
Endogenous Hormones ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Heading Chinese Cabbage

It studies the changes of endogenous hormones and polyamines in cytoplasmic male sterile non-heading Chinese cabbage (Brassica campestris L. ssp. chinensis Makino var. communis Tsen et Lee). Results showed that the microspore was prone to being sterile when there were lack of IAA, GA and polyamines, especially Put and abundant with ZRs and ABA in the anther. The imbalance of IAA/ZRs also easily caused the anther sterile.

Fertility and Cytomorphology of Anthers in New Cytoplasmic Male-Sterile Lines of Chinese Cabbage(Brassica campestris L. ssp. pekinensis)

2012 ◽  
Vol 30 (1) ◽  
pp. 49
Author(s):  
Zhan SHI ◽  
Zheng-Jie WAN ◽  
Yue-Jin XU ◽  
Rui-Chang ZOU ◽  
Tao HUANG ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Brassica Campestris ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

scholarly journals Development of cytoplasmic male sterile lines and restorer lines of various elite Indica Group rice cultivars using CW-CMS/Rf17 system

Rice ◽  
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Kinya Toriyama ◽  
Tomohiko Kazama ◽  
Tadashi Sato ◽  
Yoshimichi Fukuta ◽  
Masaaki Oka
Keyword(s):  
Nuclear Gene ◽  
Oryza Rufipogon ◽  
Rice Cultivars ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Cms Line

Abstract Background A cytoplasm of CW-type cytoplasmic male sterile (CMS) line is derived from Oryza rufipogon strain W1 and fertility is restored by a single nuclear gene, Rf17. We have previously reported that CW-CMS were effective for breeding CMS lines of Indica Group rice cultivars, IR 24 and IR 64. The applicability of this CW-CMS/Rf17 system to produce other elite Indica Group rice cultivars with CMS was explored. Findings Out of seven elite Indica Group rice cultivars, complete CMS lines were obtained for six cultivars: NSIC Rc 160, NSIC Rc 240, Ciherang, BRRI dhan 29, NERICA-L-19, and Pusa Basmati. The fertility of these six lines was restored when Rf17 was present. A CMS line was not obtained for the cultivar Samba Mahsuri. Conclusions The CW-CMS/Rf17 system will be useful to produce CMS lines and restorer lines of various elite Indica Group rice cultivars.

scholarly journals Characterization for Drought Tolerance and Physiological Efficiency in Novel Cytoplasmic Male Sterile Sources of Sunflower (Helianthus annuus L.)

2018 ◽  
Author(s):  
Vikrant Tyagi ◽  
Gurpreet Kaur ◽  
Prashant Kaushik ◽  
Satwinder Kaur Dhillon
Keyword(s):  
Water Stress ◽  
Drought Tolerance ◽  
Seed Yield ◽  
Female Parent ◽  
F1 Hybrids ◽  
Male Sterile ◽  
Water Regimes ◽  
Cytoplasmic Male Sterile ◽  
Cytoplasmic Effects ◽  
Biological Yield

Abstract:Sunflower is sensitive to drought and its hybrids have a limited cytoplasmic diversity. The wild cytoplasmic sources of sunflower are not well exploited to their potential for drought tolerance and hybrid development. In this respect, we carried out a Line × Tester based genetic study using 19 sunflower genotypes representing, 13 cytoplasmic male sterile (CMS) lines from wild and conventional sources, 2 maintainer lines, and 4 restorer lines. The CMS and maintainer lines were crossed with restorer lines to develop sixty one-way F1 hybrids. The parents and their hybrids were evaluated under two water regimes viz., normal irrigated and water stress. A total of twelve important plant descriptors were studied over a period of two years. The significant differences were observed between parents and hybrids in both water regimes. Hybrids were higher in average values for all the descriptors than parents. The role of female parent was more prominent in the expression of traits in hybrids as compared to male parents. The CMS sources varied significantly regarding seed yield per plant and other physiological traits. Proline content was three times higher in parents and their hybrids under water stress, and it was not correlated with any other descriptor. Accession CMS-PKU-2A was identified as the best general combiner for leaf area and specific leaf weight. Whereas, CMS-234A was the best general combiner for biological yield and photosynthetic efficiency under both the conditions. The cross combinations CMS-ARG-2A × RCR-8297, CMS-234A × P124R, and CMS-38A × P124R were found significant for biological yield, seed yield and oil content under both environments. Overall, this study provides useful information about the cytoplasmic effects on important sunflower traits and drought stress tolerance when used in the different combinations.

scholarly journals Breeding of Indica glutinous cytoplasmic male sterile line WX209A via CRISPR/Cas9 mediated genomic editing

2019 ◽  
Vol 55 (No. 3) ◽  
pp. 93-100 ◽  
Author(s):  
Xin Wang ◽  
Yue Han ◽  
Xuan Feng ◽  
Yun-Zhen Li ◽  
Bao-Xiang Qin ◽  
...  
Keyword(s):  
Amylose Content ◽  
Rice Variety ◽  
High Yield ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
Cytoplasmic Male Sterile ◽  
Glutinous Rice ◽  
Short Period ◽  
Cms Line

Glutinous cytoplasmic male sterile (CMS) line is necessary to select hybrid glutinous rice combination with high yield and quality. To develop glutinous CMS with low amylose content, in this study, we firstly knocked out the granule-bound starch synthase OsWaxy in 209B using CRISPR/Cas9 mediated genome editing technology and successfully obtained a glutinous maintainer line WX209B. Comparing with maintainer line 209B, WX209B showed decreased amylose contents and similar agronomic characters. And then, through one generation of hybridization and two generations of backcrossing with WX209B as the male parent and 209A as the female parent, the glutinous CMS line WX209A was successfully achieved. Our study provides a strategy to efficiently breed for the glutinous cytoplasmic male sterile line by combining CRISPR/Cas9-mediated gene editing technology with conventional backcross breeding method in a short period, which prepares the ground for further breeding of hybrid glutinous rice variety.  

Sign in / Sign up

Export Citation Format

Share Document