Susceptibility of Japanese pears to low concentrations of ethylene during storage

2007 ◽  
Vol 47 (12) ◽  
pp. 1480 ◽  
Author(s):  
M. J. Szczerbanik ◽  
K. J. Scott ◽  
J. E. Paton ◽  
D. J. Best
Keyword(s):  
Carbon Dioxide ◽  
Low Cost ◽  
Pyrus Pyrifolia ◽  
Green Colour ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Internal Browning ◽  
Term Storage

The ‘Nijisseiki’ cultivar of Japanese pears (Pyrus pyrifolia) is also known as nashi in Australia. Nashi were exposed to levels of <0.005, 0.01, 0.1 and 1.0 µL/L of ethylene in air during 26 weeks storage at 0°C. Levels of ethylene as low as 0.01 µL/L increased chlorophyll loss and visual green colour. Increasing ethylene levels also increased softening and internal browning, although flesh spot decay was reduced in the presence of ethylene. While it would be worthwhile to remove ethylene during long-term storage of ‘Nijisseiki’ in air, another alternative, adding 2% carbon dioxide to the atmosphere, is suggested as a possible low cost means to overcome the ripening effect of ethylene.

Effects of Aquifer Parameters on Long-Term Storage of Carbon Dioxide in Saline Aquifers

2012 ◽  
Author(s):  
Andrii Erich Torn ◽  
Farshid Torabi ◽  
Koorosh Asghari ◽  
Mehdi Mohammadpoor
Keyword(s):  
Carbon Dioxide ◽  
Saline Aquifers ◽  
Aquifer Parameters ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Biological Parts for Plant Biodesign to Enhance Land-Based Carbon Dioxide Removal

2021 ◽  
Vol 2021 ◽  
pp. 1-22
Author(s):  
Xiaohan Yang ◽  
Degao Liu ◽  
Haiwei Lu ◽  
David J. Weston ◽  
Jin-Gui Chen ◽  
...  
Keyword(s):  
Climate Change ◽  
Carbon Dioxide ◽  
Carbon Allocation ◽  
Value Added ◽  
Carbon Dioxide Removal ◽  
Grand Challenge ◽  
Terrestrial Plants ◽  
Long Term Storage ◽  
Term Storage

A grand challenge facing society is climate change caused mainly by rising CO2 concentration in Earth’s atmosphere. Terrestrial plants are linchpins in global carbon cycling, with a unique capability of capturing CO2 via photosynthesis and translocating captured carbon to stems, roots, and soils for long-term storage. However, many researchers postulate that existing land plants cannot meet the ambitious requirement for CO2 removal to mitigate climate change in the future due to low photosynthetic efficiency, limited carbon allocation for long-term storage, and low suitability for the bioeconomy. To address these limitations, there is an urgent need for genetic improvement of existing plants or construction of novel plant systems through biosystems design (or biodesign). Here, we summarize validated biological parts (e.g., protein-encoding genes and noncoding RNAs) for biological engineering of carbon dioxide removal (CDR) traits in terrestrial plants to accelerate land-based decarbonization in bioenergy plantations and agricultural settings and promote a vibrant bioeconomy. Specifically, we first summarize the framework of plant-based CDR (e.g., CO2 capture, translocation, storage, and conversion to value-added products). Then, we highlight some representative biological parts, with experimental evidence, in this framework. Finally, we discuss challenges and strategies for the identification and curation of biological parts for CDR engineering in plants.

scholarly journals Is adipose tissue suitable for detection of (synthetic) cannabinoids? A comparative study analyzing antemortem and postmortem specimens following pulmonary administration of JWH-210, RCS-4, as well as ∆9-tetrahydrocannabinol to pigs

2020 ◽  
Vol 94 (10) ◽  
pp. 3421-3431
Author(s):  
Nadine Schaefer ◽  
Frederike Nordmeier ◽  
Ann-Katrin Kröll ◽  
Christina Körbel ◽  
Matthias W. Laschke ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Synthetic Cannabinoids ◽  
Standard Addition ◽  
Storage Conditions ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Concentration Changes ◽  
Term Storage ◽  
Plateau Level

Abstract Examining fatal poisonings, chronic exposure may be reflected by the concentration in tissues known for long-term storage of drugs. Δ9-tetrahydrocannabinol (THC) persists in adipose tissue (AT), but sparse data on synthetic cannabinoids (SC) are available. Thus, a controlled pig study evaluating antemortem (AM) disposition and postmortem (PM) concentration changes of the SC 4-ethylnaphthalene-1-yl-(1-pentylindole-3-yl)methanone (JWH-210) and 2-(4-methoxyphenyl)-1-(1-pentyl-indole-3-yl)methanone (RCS-4) as well as THC in AT was performed. The drugs were administered pulmonarily (200 µg/kg body weight) to twelve pigs. Subcutaneous (s.c.) AT specimens were collected after 15 and 30 min and then hourly up to 8 h. At the end, pigs were sacrificed and s.c., perirenal, and dorsal AT specimens were collected. The carcasses were stored at room temperature (RT; n = 6) or 4 °C (n = 6) and specimens were collected after 24, 48, and 72 h. After homogenization in acetonitrile and standard addition, LC–MS/MS was performed. Maximum concentrations were reached 0.5–2 h after administration amounting to 21 ± 13 ng/g (JWH-210), 24 ± 13 ng/g (RCS-4), and 22 ± 20 ng/g (THC) and stayed at a plateau level. Regarding the metabolites, very low concentrations of N-hydroxypentyl-RCS-4 (HO-RCS-4) were detected from 0.5 to 8 h. PM concentrations of parent compounds did not change significantly (p > 0.05) over time under both storage conditions. Concentrations of HO-RCS-4 significantly (p < 0.05) increased in perirenal AT during storage at RT. These results suggest a rapid distribution and persistence in s.c. AT. Furthermore, AT might be resistant to PM redistribution of parent compounds. However, significant PM increases of metabolite concentrations might be considered in perirenal AT.

scholarly journals Capture and Purification of Plant Genomic DNA on a Readily-available Cellulose Matrix

2017 ◽  
Author(s):  
Megan M. Thompson ◽  
Estelle M. Hrabak
Keyword(s):  
Arabidopsis Thaliana ◽  
Nucleic Acids ◽  
Plant Species ◽  
Efficient Method ◽  
Genomic Dna ◽  
Low Cost ◽  
Long Term Storage ◽  
Cellulose Matrix ◽  
Term Storage

AbstractWhatman FTA ®Cards are a fast and efficient method for capturing and storing nucleic acids but are cost-prohibitive for some researchers. We developed a method that substitutes readily-available cellulose-based paper and homemade washing buffer for commercial FTA ®Cards and FTA ®Purification Reagent. This method is suitable for long-term storage of DNA from many plant species, including Arabidopsis thaliana, prior to purification and PCR.Method SummaryHere we report a low-cost method for long-term storage of plant genomic DNA on a readily available cellulose matrix.

scholarly journals Elevated Carbon Dioxide Storage of `Anjou' Pears Using Purge-controlled Atmosphere

HortScience ◽  
1994 ◽  
Vol 29 (4) ◽  
pp. 299-301 ◽  
Author(s):  
Stephen R. Drake
Keyword(s):  
Carbon Dioxide ◽  
Elevated Carbon Dioxide ◽  
Controlled Atmosphere ◽  
Refrigerated Storage ◽  
Long Term Storage ◽  
Keeping Quality ◽  
Flow Through ◽  
Computer Controlled ◽  
Term Storage

`Anjou' pears (Pyrus communis L.) were placed in controlled-atmosphere (CA) storage immediately after harvest (<24 hours) or after a 10-day delay in refrigerated storage, and held there for 9 months at 1C. Oxygen in all atmospheres was 1.5% and CO2 was at either 1% or 3%. Atmospheres in the flow-through system were computer-controlled at ±0.1%. After removal from CA storage, pears were evaluated immediately and after ripening at 21C for 8 days. Pears stored in 3% CO2 were firmer, greener, and displayed less scald, internal breakdown, and stem-end decay than pears stored in 1% CO2. In addition, no internal discoloration of `Anjou' pears was evident when held with 3% CO2. `Anjou' pears held in 3%. CO2 retained the ability to ripen after long-term storage. A 10-day delay in atmosphere establishment had little or no influence on the long-term keeping quality or ripening ability of `Anjou' pears.

scholarly journals Preservation of Avian Cells at Sub-zero Temperatures

1979 ◽  
Vol 32 (5) ◽  
pp. 475 ◽  
Author(s):  
N Ratnamohan ◽  
PB Spradbrow
Keyword(s):  
Dimethyl Sulfoxide ◽  
Cryoprotective Agent ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Short And Long Term ◽  
Term Storage

The cryoprotective agents dimethyl sulfoxide (DMSO), glycerol, polyvinylpyrrolidone (PVP) and dextran were evaluated for their ability to protect avian cells during storage at sub-zero temperatures. DMSO was the most effective cryoprotective agent for the short- and long-term storage of avian cells and glycerol was also effective when used at low concentrations. PVP and dextran did not protect avian cells during storage in our experiments. Primary chicken cells and avian cells at higher passage levels were successfully recovered after storage with DMSO for periods ranging from 4 to 12 months.

Evaluation of a low-cost procedure for sampling, long-term storage, and extraction of RNA from blood for qPCR analyses

2015 ◽  
Vol 53 (8) ◽  
Author(s):  
Rasmus B. Mærkedahl ◽  
Hanne Frøkiær ◽  
Lotte Lauritzen ◽  
Stine B. Metzdorff
Keyword(s):  
Gene Expression ◽  
Clinical Trials ◽  
Low Cost ◽  
Rna Extraction ◽  
Initial Step ◽  
Rna Integrity ◽  
Long Term Storage ◽  
Significant Cost Reduction ◽  
Term Storage

AbstractIn large clinical trials, where RNA cannot be extracted immediately after sampling, preserving RNA in whole blood is a crucial initial step in obtaining robust qPCR data. The current golden standard for RNA preservation is costly and designed for time-consuming column-based RNA-extraction. We investigated the use of lysis buffer for long-term storage of blood samples for qPCR analysis.Blood was collected from 13 healthy adults and diluted in MagMAX lysis/binding solution or PAXgene Blood RNA tubes and stored at –20 °C for 0, 1, or 4 months before RNA extraction by the matching method. RNA integrity, yield and purity were evaluated and the methods were compared by subsequent analyses of the gene expression levels ofThe MagMAX system extracted 2.3–2.8 times more RNA per mL blood, with better performance in terms of purity, and with comparable levels of integrity relative to the PAXgene system. Gene expression analysis using qPCR of: The MagMAX system can be used for storage of human blood for up to 4 months and is equivalent to the PAXgene system for RNA extraction. It furthermore, provides a means for significant cost reduction in large clinical trials.

Comparative Analysis of the Effect of Immersion of Porous Silicon in Aqueous Solutions of Li and Fe Salts on the Stability, Peak Position and Intensity of its Photoluminescence

2018 ◽  
Vol 386 ◽  
pp. 75-79
Author(s):  
Nikolay G. Galkin ◽  
Dmitrii Tkhyarbonovich Yan ◽  
Konstantin N. Galkin ◽  
Evgeniy Anatolievich Chusovitin ◽  
Mikhail Victorovich Bozhenko
Keyword(s):  
Porous Silicon ◽  
Aqueous Solutions ◽  
Blue Shift ◽  
Peak Position ◽  
Maximum Increase ◽  
Long Term Storage ◽  
Low Concentrations ◽  
The Stability ◽  
Term Storage

In the process of comparative studies of immersing layers of porous silicon (PS) in aqueous solutions of LiBr and Fe (NO3)3 with subsequent long-term storage up to 150 days, it is established that there exists: (1) the range of concentrations of LiBr (S/2 - S/4) and Fe (NO3)3 (0.2 M), which provide the maximum increase in the intensity of PL; (2) at low concentrations of both salts, a blue shift of the PL peaks and an increase in their intensity are observed during the long-term storage, which is associated with a decrease in the size of the NC in the PS and the influence of silicon bonds with lithium or iron ions; (3) full protection of the PS layer is observed in case of immersion in Fe (NO3)3 with a concentration of 0.7M - 0.8M.

scholarly journals Carbon Dioxide Capture and Storage

MRS Bulletin ◽  
2008 ◽  
Vol 33 (4) ◽  
pp. 303-305 ◽  
Author(s):  
Sally M. Benson ◽  
Franklin M. Orr
Keyword(s):  
Carbon Dioxide ◽  
Carbon Dioxide Capture ◽  
Long Term Storage ◽  
Sea Bottom ◽  
Deep Underground ◽  
Basic Approaches ◽  
And Storage ◽  
Geological Formations ◽  
Term Storage

Reducing CO2 emissions from the use of fossil fuel is the primary purpose of carbon dioxide capture and storage (CCS). Two basic approaches to CCS are available.1,2 In one approach, CO2 is captured directly from the industrial source, concentrated into a nearly pure form, and then pumped deep underground for long-term storage (see Figure 1). As an alternative to storage in underground geological formations, it has also been suggested that CO2 could be stored in the ocean. This could be done either by dissolving it in the mid-depth ocean (1–3 km) or by forming pools of CO2 on the sea bottom where the ocean is deeper than 3 km and, consequently, CO2 is denser than seawater. The second approach to CCS captures CO2directly from the atmosphere by enhancing natural biological processes that sequester CO2 in plants, soils, and marine sediments. All of these options for CCS have been investigated over the past decade, their potential to mitigate CO2 emissions has been evaluated,1 and several summaries are available.1,3,4

Sign in / Sign up

Export Citation Format

Share Document