Assessment of cultured fish hepatocytes for studying cellular uptake and (eco)toxicity of nanoparticles

2010 ◽  
Vol 7 (1) ◽  
pp. 36 ◽  
Author(s):  
Tessa M. Scown ◽  
Rhys M. Goodhead ◽  
Blair D. Johnston ◽  
Julian Moger ◽  
Mohammed Baalousha ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Metal Oxide Nanoparticles ◽  
Aquatic Organisms ◽  
Engineered Nanoparticles ◽  
Toxic Effects ◽  
Toxicity Screening ◽  
Glutathione S Transferase ◽  
The Sustainable Development ◽  
Vitro Model

Environmental context. The production and application of engineered nanoparticles is rapidly increasing, and development of suitable models for screening nanoparticles for possible toxic effects is essential to protect aquatic organisms and support the sustainable development of the nanotechnology industry. Here, the suitability of isolated rainbow trout hepatocytes was assessed for high through-put toxicity screening of nanoparticles and for studying uptake of nanoparticles into cells. Abstract. Relatively little is known regarding the fate and possible toxic effects of engineered nanoparticles (ENPs) in the aquatic environment. We assessed the suitability of isolated trout hepatocytes for high throughput toxicity screening of ENPs, exposing them to a variety of metal and metal oxide nanoparticles and their bulk counterparts. We found no effects of the ENPs on cell viability, or on lipid peroxidation, with the exception of exposure to ZnO nanoparticles, or on glutathione-S-transferase (GST) levels, for exposure concentrations up to 500 μg mL–1. All ENPs, however, were internalised in the cultured hepatocytes, as shown by coherent anti-Stokes Raman scattering (CARS) as an imaging technique. Our findings suggest that fish hepatocyte cultures are suitable for studies investigating the cellular uptake of ENPs, but they do not appear to be sensitive to ENP exposure and thus not a good in vitro model for nanoparticle toxicity screening.

scholarly journals Assessing the Immunosafety of Engineered Nanoparticles with a Novel in Vitro Model Based on Human Primary Monocytes

2016 ◽  
Vol 8 (42) ◽  
pp. 28437-28447 ◽  
Author(s):  
Yang Li ◽  
Paola Italiani ◽  
Eudald Casals ◽  
Dirk Valkenborg ◽  
Inge Mertens ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Engineered Nanoparticles ◽  
Model Based ◽  
Vitro Model

Bovine chromaffin cells as in vitro model for the study of the non cholinergic toxic effects of the organophosphorus compound

1996 ◽  
Vol 88 ◽  
pp. 9-10
Author(s):  
Miguel A. Sogorb ◽  
Sonia Bas ◽  
Luis Miguel Gutiérrez ◽  
Eugenio Vilanova ◽  
Salvador Viniegra
Keyword(s):  
Organophosphorus Compound ◽  
Chromaffin Cells ◽  
In Vitro Model ◽  
Toxic Effects ◽  
Bovine Chromaffin Cells ◽  
Vitro Model

An in vitro model of human airway epithelium (EpiAirway) for in vitro metabolism and toxicity screening

2007 ◽  
Vol 172 ◽  
pp. S79 ◽  
Author(s):  
Patrick Hayden ◽  
Joseph Kubilus ◽  
Helena Kandárová ◽  
Mitchell Klausner ◽  
George Jackson ◽  
...  
Keyword(s):  
Airway Epithelium ◽  
In Vitro Model ◽  
In Vitro Metabolism ◽  
Toxicity Screening ◽  
Human Airway ◽  
Human Airway Epithelium ◽  
Vitro Model

scholarly journals Rho family GTPase Rnd2 interacts and co-localizes with MgcRacGAP in male germ cells

2003 ◽  
Vol 372 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Nathalie NAUD ◽  
Aminata TOURÉ ◽  
Jianfeng LIU ◽  
Charles PINEAU ◽  
Laurence MORIN ◽  
...  
Keyword(s):  
Germ Cells ◽  
In Vitro Model ◽  
Cell Types ◽  
Glutathione S Transferase ◽  
Rac Gtpase ◽  
Male Germ Cells ◽  
Rho Family ◽  
Vitro Model ◽  
Null Mutant Mice

The male-germ-cell Rac GTPase-activating protein gene (MgcRacGAP) was initially described as a human RhoGAP gene highly expressed in male germ cells at spermatocyte stage, but exhibits significant levels of expression in most cell types. In somatic cells, MgcRacGAP protein was found to both concentrate in the midzone/midbody and be required for cytokinesis. As a RhoGAP, MgcRacGAP has been proposed to down-regulate RhoA, which is localized to the cleavage furrow and midbody during cytokinesis. Due to embryonic lethality in MgcRacGAP-null mutant mice and to the lack of an in vitro model of spermatogenesis, nothing is known regarding the role and mode of action of MgcRacGAP in male germ cells. We have analysed the expression, subcellular localization and molecular interactions of MgcRacGAP in male germ cells. Whereas MgcRacGAP was found only in spermatocytes and early spermatids, the widespread RhoGTPases RhoA, Rac1 and Cdc42 (which are, to various extents, in vitro substrates for MgcRacGAP activity) were, surprisingly, not detected at these stages. In contrast, Rnd2, a Rho family GTPase-deficient G-protein was found to be co-expressed with MgcRacGAP in spermatocytes and spermatids. MgcRacGAP was detected in the midzone of meiotic cells, but also, unexpectedly, in the Golgi-derived pro-acrosomal vesicle, co-localizing with Rnd2. In addition, a stable Rnd2–MgcRacGAP molecular complex could be evidenced by glutathione S-transferase pull-down and co-immunoprecipitation experiments. We conclude that Rnd2 is a probable physiological partner of MgcRacGAP in male germ cells and we propose that MgcRacGAP, and, quite possibly, other RhoGAPs, may participate in signalling pathways involving Rnd family proteins.

scholarly journals Use of the in vitro model for the evaluation of toxic effects of metabolites produced by fungi

2007 ◽  
Author(s):  
Z. Kováčiková ◽  
E. Piecková ◽  
E. Tátrai ◽  
Z. Pivovarová ◽  
M. Mataušic-Pišl
Keyword(s):  
In Vitro Model ◽  
Toxic Effects ◽  
Vitro Model

scholarly journals Sublethal Toxic Effects and Induction of gGutathione S-transferase by Short-Chain Chlorinated Paraffins (SCCPs) and C-12 alkane (dodecane) in Xenopus laevis Frog Embryos

2006 ◽  
Vol 75 (1) ◽  
pp. 115-122 ◽  
Author(s):  
B. Burýšková ◽  
L. Bláha ◽  
D. Vršková ◽  
K. Šimková ◽  
B. Maršálek
Keyword(s):  
Xenopus Laevis ◽  
Developmental Toxicity ◽  
Aquatic Organisms ◽  
Short Chain ◽  
Toxic Effects ◽  
Industrial Chemicals ◽  
Glutathione S Transferase ◽  
Commercial Mixture ◽  
Chlorinated Paraffins ◽  
Embryotoxic Effects

Short chain chlorinated paraffins (SCCPs) are important industrial chemicals with high persistence in the environment but poorly characterized ecotoxicological effects. We studied embryotoxic effects of commercial mixture of SCCP (carbon length C-12, 56% of chlorine; CP56-12) and non-chlorinated n-alkane (dodecane, C-12) in the 96h Frog Embryo Teratogenesis Assay - Xenopus (FETAX). Only weak lethal effects were observed for both substances (the highest tested concentration 500 mg/L of both chemicals caused up to 11% mortality). On the other hand, we observed developmental malformations and reduced embryo growth at 5 mg/l and higher concentrations. However, the effects were not related to chlorination pattern as both SCCPs and dodecane induced qualitatively similar effects. SCCPs also significantly induced phase II detoxification enzyme glutathione S-transferase (GST) in Xenopus laevis embryos even at 0.5 mg/L, and this biomarker might be used as another early warning of chronic toxic effects. Our results newly indicate significant developmental toxicity of both SCCPs and n-dodecane to aquatic organisms along with inductions of specific biochemical toxicity mechanisms.

Doxorubicin-Mediated Toxic Effects Are Mediated Via NO/eNOS in a Novel 3D in Vitro Model of the Human Heart

2016 ◽  
Vol 100 ◽  
pp. S142
Author(s):  
Liudmila Polonchuk ◽  
Mamta Chabria ◽  
Michael J. Davies ◽  
Carmine Gentile
Keyword(s):  
Human Heart ◽  
In Vitro Model ◽  
Toxic Effects ◽  
3D In Vitro Model ◽  
Vitro Model

scholarly journals Comprehensive phenotyping and transcriptome profiling to study nanotoxicity in C. elegans

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8684 ◽  
Author(s):  
Charles Viau ◽  
Orçun Haçariz ◽  
Farial Karimian ◽  
Jianguo Xia
Keyword(s):  
Expression Profiles ◽  
Metal Oxide Nanoparticles ◽  
Gene Expression Profiles ◽  
Mapk Signaling ◽  
Engineered Nanoparticles ◽  
Toxic Effects ◽  
Receptor Interaction ◽  
Suitable Model ◽  
Reproductive Process ◽  
C Elegans

Engineered nanoparticles are used at an increasing rate in both industry and medicine without fully understanding their impact on health and environment. The nematode Caenorhabditis elegans is a suitable model to study the toxic effects of nanoparticles as it is amenable to comprehensive phenotyping, such as locomotion, growth, neurotoxicity and reproduction. In this study, we systematically evaluated the effects of silver (Ag) and five metal oxide nanoparticles: SiO2, CeO2, CuO, Al2O3 and TiO2. The results showed that Ag and SiO2 exposures had the most toxic effects on locomotion velocity, growth and reproduction, whereas CeO2, Al2O3 and CuO exposures were mostly neurotoxic. We further performed RNAseq to compare the gene expression profiles underlying Ag and SiO2toxicities. Gene set enrichment analyses revealed that exposures to Ag and SiO2consistently downregulated several biological processes (regulations in locomotion, reproductive process and cell growth) and pathways (neuroactive ligand-receptor interaction, wnt and MAPK signaling, etc.), with opposite effects on genes involved in innate immunity. Our results contribute to mechanistic insights into toxicity of Ag and SiO2 nanoparticles and demonstrated that C. elegans as a valuable model for nanotoxicity assessment.

Sign in / Sign up

Export Citation Format

Share Document