Arabidopsis thaliana MYB75/PAP1 transcription factor induces anthocyanin production in transgenic tomato plants

2008 ◽  
Vol 35 (7) ◽  
pp. 606 ◽  
Author(s):  
Diana Lucia Zuluaga ◽  
Silvia Gonzali ◽  
Elena Loreti ◽  
Chiara Pucciariello ◽  
Elena Degl'Innocenti ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Transgenic Plants ◽  
Anthocyanin Biosynthesis ◽  
High Irradiance ◽  
Transgenic Tomato ◽  
Vascular Bundles ◽  
Tomato Plants ◽  
Transgenic Tomato Plants ◽  
Anthocyanin Production

Tomato (Solanum lycopersicum L.) cv. Micro-Tom plants were transformed with the Arabidopsis thaliana (L.)Heyhn. MYB75/PAP1 (PRODUCTION OF ANTHOCYANIN PIGMENT 1) gene. This gene encodes for a well known transcription factor, which is involved in anthocyanin production and is modulated by light and sucrose. Transgenic tomato plants expressing AtMYB75 were characterised by a significantly higher anthocyanin production in leaves, stems, roots and flowers under normal growth conditions. Further, they also exhibited anthocyanins in fruits. Anthocyanin accumulation was not widespread but took place in specific groups of cells located in epidermal or cortical regions or in proximity of vascular bundles. In all the organs of the transgenic plants, where AtMYB75 overexpression was determined, a clear increase in the accumulation of DFR (DIHYDROFLAVONOL 4-REDUCTASE) transcript was also detected. The expression of the tomato MYB-gene ANT1 (ANTHOCYANIN1), which had previously been identified as a transcriptional endogenous regulator of anthocyanin biosynthesis, was not altered. The higher basal content of anthocyanins in the leaves of the transgenic plants could be further increased in the presence of high light conditions and contributed to mitigate photobleaching damages under high irradiance.

scholarly journals Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

2019 ◽  
Author(s):  
Yuting Qi ◽  
Caihong Gu ◽  
Xingjun Wang ◽  
Shiqing Gao ◽  
Changsheng Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transgenic Plants ◽  
Anthocyanin Biosynthesis ◽  
Ectopic Expression ◽  
Myb Transcription Factor ◽  
Anthocyanin Synthesis ◽  
Eutrema Salsugineum ◽  
Tobacco Transgenic Plants ◽  
Myb Genes ◽  
Anthocyanin Production

Abstract Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum.Result: Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum, which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that EsMYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by EsMYB90 in 35S:EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that EsMYB90 promoted expression of early (PAL, CHS, and CHI) and late (DFR, ANS, and UFGT) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S:EsMYB90 tobacco transgenic plants.Conclusions: Our results indicated that EsMYB90 is a novel MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants.

MODIFIED COMPOSITION OF MICROBIAL COMMUNITY IN THE RHIZOSPHERE OF TRANSGENIC TOMATO PLANTS WITH A GLYCINE BETAINE SYNTESIS GENE

2020 ◽  
pp. 18-29
Author(s):  
A.A. ANTONOV ◽  
◽  
E.N. BARANOVA ◽  
A.A. GULEVICH ◽  
L.V. KURENINA ◽  
...  
Keyword(s):  
Microbial Community ◽  
Transgenic Plants ◽  
Root Zone ◽  
Microbial Community Composition ◽  
Transgenic Tomato ◽  
Choline Oxidase ◽  
Soil Culture ◽  
Oxidase Gene ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

The change in the composition of soil microbiota as a result of the cultivation of various crops in agrocenoses is currently of great interest. The authors studied the effect of the root system of transgenic tomato plants (Solanum lycopersicum L.) grown in soil culture on the microbial community in the rhizosphere. The results showed that as a result of the cultivation of transgenic plants with the choline oxidase gene, the microbial community composition in the rhizosphere has changed significantly. A significant increase in the proportion (73%) and species diversity (Shannon index 2.25) of actinobacteria in the soil root zone of tomato transgenic codA plants as compared with control plants (10% and 0.95, respectively) has been revealed. The content of pseudomonads and micromycetes is significantly reduced (25 and 12% in transgenic plants; 70 and 81% in control plants, respectively). Thus, genetically modified plants are able to influence the microbial community structure in the rhizosphere.

scholarly journals Transgenic Plants Expressing Geminivirus Movement Proteins: Abnormal Phenotypes and Delayed Infection by Tomato mottle virus in Transgenic Tomatoes Expressing the Bean dwarf mosaic virus BV1 or BC1 Proteins

2000 ◽  
Vol 13 (3) ◽  
pp. 297-308 ◽  
Author(s):  
Yu-Ming Hou ◽  
Rick Sanders ◽  
Virgina M. Ursin ◽  
Robert L. Gilbertson
Keyword(s):  
Transgenic Plants ◽  
Mosaic Virus ◽  
Viral Disease ◽  
Transgenic Tomato ◽  
Wild Type ◽  
Significant Delay ◽  
Tomato Plants ◽  
Movement Proteins ◽  
Transgenic Tomato Plants ◽  
Bean Dwarf Mosaic Virus

Transgenic tomato plants expressing wild-type or mutated BV1 or BC1 movement proteins from Bean dwarf mosaic virus (BDMV) were generated and examined for phenotypic effects and resistance to Tomato mottle virus (ToMoV). Fewer transgenic plants were recovered with the wild-type or mutated BC1 genes, compared with the wild-type or mutated BV1 genes. Transgenic tomato plants expressing the wild-type or mutated BV1 proteins appeared normal. Interestingly, although BDMV induces only a symptomless infection in tomato (i.e., BDMV is not well adapted to tomato), transgenic tomato plants expressing the BDMV BC1 protein showed a viral disease-like phenotype (i.e., stunted growth, and leaf mottling, curling, and distortion). This suggests that the symptomless phenotype of BDMV in tomato is not due to a host-specific defect in the BC1 protein. One transgenic line expressing the BC1 gene did not show the viral disease-like phenotype. This was associated with a deletion in the 3′ region of the gene, which resulted in expression of a truncated BC1 protein. Several R0 plants, expressing either wild-type or mutated BV1 or BC1 proteins, showed a significant delay in ToMoV infection, compared with non-transformed plants. R1 progeny plants also showed a significant delay in ToMoV infection, but this delay was less than that in the R0 parents. These results also demonstrate that expression of viral movement proteins, in transgenic plants, can have deleterious effects on various aspects of plant development.

scholarly journals Transgenic Tomato Plants Overexpressing Tyramine N-Hydroxycinnamoyltransferase Exhibit Elevated Hydroxycinnamic Acid Amide Levels and Enhanced Resistance to Pseudomonas syringae

2014 ◽  
Vol 27 (10) ◽  
pp. 1159-1169 ◽  
Author(s):  
Laura Campos ◽  
Purificación Lisón ◽  
María Pilar López-Gresa ◽  
Ismael Rodrigo ◽  
Laura Zacarés ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Pseudomonas Syringae ◽  
Acid Amide ◽  
Hydroxycinnamic Acid ◽  
Transgenic Tomato ◽  
Tomato Plants ◽  
Antibacterial And Antifungal Activities ◽  
Enhanced Resistance ◽  
Transgenic Tomato Plants ◽  
Key Enzyme

Hydroxycinnamic acid amides (HCAA) are secondary metabolites involved in plant development and defense that have been widely reported throughout the plant kingdom. These phenolics show antioxidant, antiviral, antibacterial, and antifungal activities. Hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyl transferase (THT) is the key enzyme in HCAA synthesis and is induced in response to pathogen infection, wounding, or elicitor treatments, preceding HCAA accumulation. We have engineered transgenic tomato plants overexpressing tomato THT. These plants displayed an enhanced THT gene expression in leaves as compared with wild type (WT) plants. Consequently, leaves of THT-overexpressing plants showed a higher constitutive accumulation of the amide coumaroyltyramine (CT). Similar results were found in flowers and fruits. Moreover, feruloyltyramine (FT) also accumulated in these tissues, being present at higher levels in transgenic plants. Accumulation of CT, FT and octopamine, and noradrenaline HCAA in response to Pseudomonas syringae pv. tomato infection was higher in transgenic plants than in the WT plants. Transgenic plants showed an enhanced resistance to the bacterial infection. In addition, this HCAA accumulation was accompanied by an increase in salicylic acid levels and pathogenesis-related gene induction. Taken together, these results suggest that HCAA may play an important role in the defense of tomato plants against P. syringae infection.

scholarly journals Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

2020 ◽  
Author(s):  
Yuting Qi ◽  
Caihong Gu ◽  
Xingjun Wang ◽  
Shiqing Gao ◽  
Changsheng Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transgenic Plants ◽  
Anthocyanin Biosynthesis ◽  
Ectopic Expression ◽  
Myb Transcription Factor ◽  
Anthocyanin Synthesis ◽  
Eutrema Salsugineum ◽  
Tobacco Transgenic Plants ◽  
Myb Genes ◽  
Anthocyanin Production

Abstract Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum.Result: Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum, which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that EsMYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by EsMYB90 in 35S:EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that EsMYB90 promoted expression of early (PAL, CHS, and CHI) and late (DFR, ANS, and UFGT) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S:EsMYB90 tobacco transgenic plants.Conclusions: Our results indicated that EsMYB90 is a MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants.

scholarly journals Transgenic Tomato Plants Expressing Satellite RNA Are Tolerant to Some Strains of Cucumber Mosaic Virus

1994 ◽  
Vol 119 (3) ◽  
pp. 642-647 ◽  
Author(s):  
P.B. McGarvey ◽  
M.S. Montasser ◽  
J.M. Kaper
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Unit Length ◽  
Transgenic Tomato ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Satellite Rna ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

Transgenic tomato plants (Lycopersicon esculentum Mill.) expressing cucumber mosaic virus (CMV) satellite RNA fused to a gene for β-glucuronidase were produced using Agrobacterium-mediated transformation. The R1 progeny of self-crossed R0 plants were challenge-inoculated with virion or RNA preparations of CMV or tomato aspermy virus (TAV). The transgenic plants challenged with CMV-1 showed mild disease symptoms in the first 2 weeks postchallenge followed by a decrease in symptoms, resulting in little difference between the transgenic and uninfected control group by the fourth week. Enzyme-linked immunosorbent assay results showed about a 10-fold decrease in virus accumulation in the transgenic plants compared to controls. Tolerance was evident only in plants that contained the recombinant insert and produced mature unit-length satellite RNA after CMV infection. Plants challenged with TAV showed no significant tolerance to virus-induced symptoms.

scholarly journals Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

2020 ◽  
Author(s):  
Yuting Qi ◽  
Caihong Gu ◽  
Xingjun Wang ◽  
Shiqing Gao ◽  
Changsheng Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transgenic Plants ◽  
Anthocyanin Biosynthesis ◽  
Ectopic Expression ◽  
Myb Transcription Factor ◽  
Anthocyanin Synthesis ◽  
Eutrema Salsugineum ◽  
Tobacco Transgenic Plants ◽  
Myb Genes ◽  
Anthocyanin Production

Abstract Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum . Result: Here we report the identification of an important anthocyanin biosynthesis regulator Es MYB90 from Eutrema salsugineum , which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that Es MYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by Es MYB90 in 35S : EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that Es MYB90 promoted expression of early ( PAL , CHS , and CHI ) and late ( DFR , ANS , and UFGT ) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S : EsMYB90 tobacco transgenic plants. Conclusions: Our results indicated that Es MYB90 is a MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants.

scholarly journals Efficacy of Endogenous Satellite Expression to Confer Resistance to CMV in Satellite Transgenic Tomato under Field Conditions

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 569c-569
Author(s):  
John R. Stommel ◽  
Marie E. Tousignant ◽  
Thanda Wai ◽  
Jacobus M. Kaper
Keyword(s):  
Transgenic Plants ◽  
Field Tests ◽  
Field Trials ◽  
Transgenic Tomato ◽  
Field Conditions ◽  
Test Plot ◽  
Tomato Plants ◽  
Transgenic Tomato Plants ◽  
Transgenic Lines ◽  
Crop Disease

Viral satellite RNA associated with cucumber mosaic virus (CMV) is know to modulate CMV symptomology. Virulent CMV associated RNA 5 (CARNA 5) satellites may intensify crop disease. Naturally occurring variants of these satellites, however, attenuate CMV symptoms. Satellite transgenic tomato plants expressing the S-CARNA 5 or 1-CARNA 5 ameliorating forms of the satellite were evaluated under simulated CMV epidemic conditions in USDA–APHIS approved field trials. Trials conducted at Beltsville, Md., in 1994 and 1995 demonstrated that CMV can be effectively controlled under field conditions in satellite transgenic plants. Yields of transgenic lines infected with CMV were 50%–65% greater than that of non-transgenic infected controls. Yields of noninfected transgenic lines ranged from 5% greater than, to 33% less than, noninfected nontransgenic controls. Expression of CARNA 5 in inoculated transgenic plants greatly reduced CMV foliar symptoms and virus titers when compared to inoculated control plants. Levels of CARNA 5 were detected at varying levels in infected transgenic plants throughout the growing season. Virus or satellite was not detected in samples collected from tomato border plants and weeds growing inside and outside a nonhost crop border surrounding the test plot. Field tests conducted in 1996 will evaluate transgenic tomato plants with a double construct coding for the CMV coat protein gene and 1-CARNA 5 satellite.

scholarly journals Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

2020 ◽  
Author(s):  
Yuting Qi ◽  
Caihong Gu ◽  
Xingjun Wang ◽  
Shiqing Gao ◽  
Changsheng Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transgenic Plants ◽  
Anthocyanin Biosynthesis ◽  
Ectopic Expression ◽  
Myb Transcription Factor ◽  
Anthocyanin Synthesis ◽  
Eutrema Salsugineum ◽  
Tobacco Transgenic Plants ◽  
Myb Genes ◽  
Anthocyanin Production

Abstract Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum.Result: Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum, which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that EsMYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by EsMYB90 in 35S:EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that EsMYB90 promoted expression of early (PAL, CHS, and CHI) and late (DFR, ANS, and UFGT) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S:EsMYB90 tobacco transgenic plants.Conclusions: Our results indicated that EsMYB90 is a MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants.

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