Efficacy of Endogenous Satellite Expression to Confer Resistance to CMV in Satellite Transgenic Tomato under Field Conditions

Viral satellite RNA associated with cucumber mosaic virus (CMV) is know to modulate CMV symptomology. Virulent CMV associated RNA 5 (CARNA 5) satellites may intensify crop disease. Naturally occurring variants of these satellites, however, attenuate CMV symptoms. Satellite transgenic tomato plants expressing the S-CARNA 5 or 1-CARNA 5 ameliorating forms of the satellite were evaluated under simulated CMV epidemic conditions in USDA–APHIS approved field trials. Trials conducted at Beltsville, Md., in 1994 and 1995 demonstrated that CMV can be effectively controlled under field conditions in satellite transgenic plants. Yields of transgenic lines infected with CMV were 50%–65% greater than that of non-transgenic infected controls. Yields of noninfected transgenic lines ranged from 5% greater than, to 33% less than, noninfected nontransgenic controls. Expression of CARNA 5 in inoculated transgenic plants greatly reduced CMV foliar symptoms and virus titers when compared to inoculated control plants. Levels of CARNA 5 were detected at varying levels in infected transgenic plants throughout the growing season. Virus or satellite was not detected in samples collected from tomato border plants and weeds growing inside and outside a nonhost crop border surrounding the test plot. Field tests conducted in 1996 will evaluate transgenic tomato plants with a double construct coding for the CMV coat protein gene and 1-CARNA 5 satellite.

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MODIFIED COMPOSITION OF MICROBIAL COMMUNITY IN THE RHIZOSPHERE OF TRANSGENIC TOMATO PLANTS WITH A GLYCINE BETAINE SYNTESIS GENE

Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii ◽

10.26897/0021-342x-2020-5-18-29 ◽

2020 ◽

pp. 18-29

Author(s):

A.A. ANTONOV ◽

◽

E.N. BARANOVA ◽

A.A. GULEVICH ◽

L.V. KURENINA ◽

...

Keyword(s):

Microbial Community ◽

Transgenic Plants ◽

Root Zone ◽

Microbial Community Composition ◽

Transgenic Tomato ◽

Choline Oxidase ◽

Soil Culture ◽

Oxidase Gene ◽

Tomato Plants ◽

Transgenic Tomato Plants

The change in the composition of soil microbiota as a result of the cultivation of various crops in agrocenoses is currently of great interest. The authors studied the effect of the root system of transgenic tomato plants (Solanum lycopersicum L.) grown in soil culture on the microbial community in the rhizosphere. The results showed that as a result of the cultivation of transgenic plants with the choline oxidase gene, the microbial community composition in the rhizosphere has changed significantly. A significant increase in the proportion (73%) and species diversity (Shannon index 2.25) of actinobacteria in the soil root zone of tomato transgenic codA plants as compared with control plants (10% and 0.95, respectively) has been revealed. The content of pseudomonads and micromycetes is significantly reduced (25 and 12% in transgenic plants; 70 and 81% in control plants, respectively). Thus, genetically modified plants are able to influence the microbial community structure in the rhizosphere.

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Transgenic Plants Expressing Geminivirus Movement Proteins: Abnormal Phenotypes and Delayed Infection by Tomato mottle virus in Transgenic Tomatoes Expressing the Bean dwarf mosaic virus BV1 or BC1 Proteins

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2000.13.3.297 ◽

2000 ◽

Vol 13 (3) ◽

pp. 297-308 ◽

Cited By ~ 54

Author(s):

Yu-Ming Hou ◽

Rick Sanders ◽

Virgina M. Ursin ◽

Robert L. Gilbertson

Keyword(s):

Transgenic Plants ◽

Mosaic Virus ◽

Viral Disease ◽

Transgenic Tomato ◽

Wild Type ◽

Significant Delay ◽

Tomato Plants ◽

Movement Proteins ◽

Transgenic Tomato Plants ◽

Bean Dwarf Mosaic Virus

Transgenic tomato plants expressing wild-type or mutated BV1 or BC1 movement proteins from Bean dwarf mosaic virus (BDMV) were generated and examined for phenotypic effects and resistance to Tomato mottle virus (ToMoV). Fewer transgenic plants were recovered with the wild-type or mutated BC1 genes, compared with the wild-type or mutated BV1 genes. Transgenic tomato plants expressing the wild-type or mutated BV1 proteins appeared normal. Interestingly, although BDMV induces only a symptomless infection in tomato (i.e., BDMV is not well adapted to tomato), transgenic tomato plants expressing the BDMV BC1 protein showed a viral disease-like phenotype (i.e., stunted growth, and leaf mottling, curling, and distortion). This suggests that the symptomless phenotype of BDMV in tomato is not due to a host-specific defect in the BC1 protein. One transgenic line expressing the BC1 gene did not show the viral disease-like phenotype. This was associated with a deletion in the 3′ region of the gene, which resulted in expression of a truncated BC1 protein. Several R0 plants, expressing either wild-type or mutated BV1 or BC1 proteins, showed a significant delay in ToMoV infection, compared with non-transformed plants. R1 progeny plants also showed a significant delay in ToMoV infection, but this delay was less than that in the R0 parents. These results also demonstrate that expression of viral movement proteins, in transgenic plants, can have deleterious effects on various aspects of plant development.

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Transgenic Tomato Plants Overexpressing Tyramine N-Hydroxycinnamoyltransferase Exhibit Elevated Hydroxycinnamic Acid Amide Levels and Enhanced Resistance to Pseudomonas syringae

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-04-14-0104-r ◽

2014 ◽

Vol 27 (10) ◽

pp. 1159-1169 ◽

Cited By ~ 36

Author(s):

Laura Campos ◽

Purificación Lisón ◽

María Pilar López-Gresa ◽

Ismael Rodrigo ◽

Laura Zacarés ◽

...

Keyword(s):

Transgenic Plants ◽

Pseudomonas Syringae ◽

Acid Amide ◽

Hydroxycinnamic Acid ◽

Transgenic Tomato ◽

Tomato Plants ◽

Antibacterial And Antifungal Activities ◽

Enhanced Resistance ◽

Transgenic Tomato Plants ◽

Key Enzyme

Hydroxycinnamic acid amides (HCAA) are secondary metabolites involved in plant development and defense that have been widely reported throughout the plant kingdom. These phenolics show antioxidant, antiviral, antibacterial, and antifungal activities. Hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyl transferase (THT) is the key enzyme in HCAA synthesis and is induced in response to pathogen infection, wounding, or elicitor treatments, preceding HCAA accumulation. We have engineered transgenic tomato plants overexpressing tomato THT. These plants displayed an enhanced THT gene expression in leaves as compared with wild type (WT) plants. Consequently, leaves of THT-overexpressing plants showed a higher constitutive accumulation of the amide coumaroyltyramine (CT). Similar results were found in flowers and fruits. Moreover, feruloyltyramine (FT) also accumulated in these tissues, being present at higher levels in transgenic plants. Accumulation of CT, FT and octopamine, and noradrenaline HCAA in response to Pseudomonas syringae pv. tomato infection was higher in transgenic plants than in the WT plants. Transgenic plants showed an enhanced resistance to the bacterial infection. In addition, this HCAA accumulation was accompanied by an increase in salicylic acid levels and pathogenesis-related gene induction. Taken together, these results suggest that HCAA may play an important role in the defense of tomato plants against P. syringae infection.

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Overexpression of PbDHAR2 from Pyrus sinkiangensis in Transgenic Tomato Confers Enhanced Tolerance to Salt and Chilling Stresses

HortScience ◽

10.21273/hortsci.50.6.789 ◽

2015 ◽

Vol 50 (6) ◽

pp. 789-796 ◽

Cited By ~ 8

Author(s):

An Qin ◽

Xiaosan Huang ◽

Huping Zhang ◽

Juyou Wu ◽

Jie Yang ◽

...

Keyword(s):

Ascorbic Acid ◽

Chilling Tolerance ◽

Antioxidant Enzyme Activity ◽

Transgenic Tomato ◽

Wild Type ◽

Tomato Plants ◽

Chlorophyll Contents ◽

Transgenic Tomato Plants ◽

Transgenic Lines ◽

Redox Buffer

Ascorbic acid (AsA) is a major antioxidant and redox buffer in plants. Dehydroascorbate reductase (DHAR; EC 1.8.5.1) catalyzes the conversion of dehydroascorbate (DHA) to AsA and is crucial for AsA regeneration. In this study, we developed transgenic tomato plants that overexpressed PbDHAR2 to investigate whether PbDHAR2 could limit the deleterious effects of salt and chilling stresses. These transgenic plants contained significantly higher AsA levels than the wild-type (WT) plants. Overexpression of PbDHAR2 increased the expression of the AsA-glutathione (GSH) cycle genes in transgenic lines under salt and chilling stresses. In addition, the transgenic lines subjected to salt and chilling stresses showed higher levels of antioxidant enzyme activity, lower malondialdehyde (MDA) levels, and higher chlorophyll contents than the WT. Thus, our results demonstrate that the regulation of PbDHAR2 during AsA regeneration contributes to enhanced salt and chilling tolerance in tomato.

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Transgenic Tomato Plants Expressing Satellite RNA Are Tolerant to Some Strains of Cucumber Mosaic Virus

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.119.3.642 ◽

1994 ◽

Vol 119 (3) ◽

pp. 642-647 ◽

Cited By ~ 19

Author(s):

P.B. McGarvey ◽

M.S. Montasser ◽

J.M. Kaper

Keyword(s):

Transgenic Plants ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Unit Length ◽

Transgenic Tomato ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Satellite Rna ◽

Tomato Plants ◽

Transgenic Tomato Plants

Transgenic tomato plants (Lycopersicon esculentum Mill.) expressing cucumber mosaic virus (CMV) satellite RNA fused to a gene for β-glucuronidase were produced using Agrobacterium-mediated transformation. The R1 progeny of self-crossed R0 plants were challenge-inoculated with virion or RNA preparations of CMV or tomato aspermy virus (TAV). The transgenic plants challenged with CMV-1 showed mild disease symptoms in the first 2 weeks postchallenge followed by a decrease in symptoms, resulting in little difference between the transgenic and uninfected control group by the fourth week. Enzyme-linked immunosorbent assay results showed about a 10-fold decrease in virus accumulation in the transgenic plants compared to controls. Tolerance was evident only in plants that contained the recombinant insert and produced mature unit-length satellite RNA after CMV infection. Plants challenged with TAV showed no significant tolerance to virus-induced symptoms.

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Arabidopsis thaliana MYB75/PAP1 transcription factor induces anthocyanin production in transgenic tomato plants

Functional Plant Biology ◽

10.1071/fp08021 ◽

2008 ◽

Vol 35 (7) ◽

pp. 606 ◽

Cited By ~ 75

Author(s):

Diana Lucia Zuluaga ◽

Silvia Gonzali ◽

Elena Loreti ◽

Chiara Pucciariello ◽

Elena Degl'Innocenti ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Transcription Factor ◽

Transgenic Plants ◽

Anthocyanin Biosynthesis ◽

High Irradiance ◽

Transgenic Tomato ◽

Vascular Bundles ◽

Tomato Plants ◽

Transgenic Tomato Plants ◽

Anthocyanin Production

Tomato (Solanum lycopersicum L.) cv. Micro-Tom plants were transformed with the Arabidopsis thaliana (L.)Heyhn. MYB75/PAP1 (PRODUCTION OF ANTHOCYANIN PIGMENT 1) gene. This gene encodes for a well known transcription factor, which is involved in anthocyanin production and is modulated by light and sucrose. Transgenic tomato plants expressing AtMYB75 were characterised by a significantly higher anthocyanin production in leaves, stems, roots and flowers under normal growth conditions. Further, they also exhibited anthocyanins in fruits. Anthocyanin accumulation was not widespread but took place in specific groups of cells located in epidermal or cortical regions or in proximity of vascular bundles. In all the organs of the transgenic plants, where AtMYB75 overexpression was determined, a clear increase in the accumulation of DFR (DIHYDROFLAVONOL 4-REDUCTASE) transcript was also detected. The expression of the tomato MYB-gene ANT1 (ANTHOCYANIN1), which had previously been identified as a transcriptional endogenous regulator of anthocyanin biosynthesis, was not altered. The higher basal content of anthocyanins in the leaves of the transgenic plants could be further increased in the presence of high light conditions and contributed to mitigate photobleaching damages under high irradiance.

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