The detection and significance of emerging insecticide resistance in mosquitoes

2018 ◽  
Vol 39 (2) ◽  
pp. 80 ◽  
Author(s):  
Nancy M Endersby-Harshman ◽  
Andrew R Weeks ◽  
Ary A Hoffmann

Mosquito-borne arboviruses are increasing in incidence around the world. Australia enjoys some protection from pests and diseases afforded by its geographic isolation coupled with strict biosecurity control at its borders. However, as the volume of global trade, travel and transport expands, risk of exotic incursions to Australia is increasing. Detection of foreign mosquitoes at airports and seaports around Australia is becoming commonplace. The Asian tiger mosquito, Aedes albopictus, which has expanded its range throughout Europe and the Americas1, has not become established in mainland Australia, but is encountered as an exotic incursion2. The yellow fever mosquito and dengue vector, Aedes. aegypti, occurs naturally in northern Queensland, but is also captured at Australia’s ports on a recurrent basis as an incursion from overseas3. Although Ae. aegypti is established in Australia, its detection as an incursion is still cause for concern. Apart from the possibility that invasive mosquitoes will carry exotic arboviruses, genetic characteristics of a foreign insect population can be very different from those observed in local mosquitoes, particularly in terms of insecticide resistance. Our recent research has shown that invading mosquitoes from overseas carry insecticide resistance alleles not found in Australia4 and our development of a global genomic database is helping us to pinpoint their source.

Author(s):  
Mara Moreno-Gómez ◽  
Rubén Bueno-Marí ◽  
Andrea Drago ◽  
Miguel A Miranda

Abstract Vector-borne diseases are a worldwide threat to human health. Often, no vaccines or treatments exist. Thus, personal protection products play an essential role in limiting transmission. The World Health Organization (WHO) arm-in-cage (AIC) test is the most common method for evaluating the efficacy of topical repellents, but it remains unclear whether AIC testing conditions recreate the mosquito landing rates in the field. This study aimed to estimate the landing rate outdoors, in an area of Europe highly infested with the Asian tiger mosquito (Aedes albopictus (Skuse, 1894, Diptera: Culididae)), and to determine how to replicate this rate in the laboratory. To assess the landing rate in the field, 16 individuals were exposed to mosquitoes in a highly infested region of Italy. These field results were then compared to results obtained in the laboratory: 1) in a 30 m3 room where nine volunteers were exposed to different mosquito abundances (ranges: 15–20, 25–30, and 45–50) and 2) in a 0.064 m3 AIC test cage where 10 individuals exposed their arms to 200 mosquitoes (as per WHO requirements). The highest mosquito landing rate in the field was 26.8 landings/min. In the room test, a similar landing rate was achieved using 15–20 mosquitoes (density: 0.50–0.66 mosquitoes/m3) and an exposure time of 3 min. In the AIC test using 200 mosquitoes (density: 3,125 mosquitoes/m3), the landing rate was 229 ± 48 landings/min. This study provides useful reference values that can be employed to design new evaluation standards for topical repellents that better simulate field conditions.


Zootaxa ◽  
2018 ◽  
Vol 4413 (1) ◽  
pp. 197 ◽  
Author(s):  
EDUARDO MARABUTO ◽  
MARIA TERESA REBELO

The Asian tiger mosquito, Aedes (Stegomyia) albopictus (Skuse), is a tropical species originally from south-eastern Asia (Hawley, 1988). It has experienced a rampant human-mediated range expansion since the 1970s to now occupy almost the whole of the tropical and subtropical areas of the world. As an eclectic haematophagous species, it attacks humans and is able to use a number of man-made and natural structures where stagnant water is present (Estrada-Franco & Craig, 1995). Eggs are able to survive for extended periods of time in complete dryness and diapause over unsuitable cold season, making it an especially resilient species (Hawley, 1988). 


2017 ◽  
Author(s):  
Eduardo Marabuto ◽  
Maria Teresa Rebelo

AbstractThe mosquito Aedes albopictus is here reported for the first time in Portugal, from the south of the country, at least 240km west of the nearest known observation in Spain. A population of more than fifty specimens was spotted within a suburban garden over seven days of survey. As an important vector of Human affecting zoonoses such as dengue, chikungunya and yellow-fever, the presence of this mosquito in Portugal now enhances the outbreak chances for such diseases.


2020 ◽  
Vol 76 (12) ◽  
pp. 4202-4209 ◽  
Author(s):  
Thomas L Schmidt ◽  
Jessica Chung ◽  
Anthony R Rooyen ◽  
Angus Sly ◽  
Andrew R Weeks ◽  
...  

2020 ◽  
Author(s):  
Lianfen Huang ◽  
Jun Li ◽  
Xinghua Su ◽  
Ruili Xie ◽  
Lilan Peng ◽  
...  

Abstract Background: Fast emerging and increasing insecticide resistance in Asian tiger mosquito, Aedes albopictus brings alarm to the dengue epidemic over the world. The genetic and transcriptional sequencing has greatly advanced our understanding of the molecular basis for this crucial adaptation, but less is known about the regulation of insecticide resistance physiology at the metabolomic level. Methods: We captured the relative abundance of 17 amino acids and 34 AcylCNs in the 3rd to 4th instar larvae of three geographically close strains of Ae. albopictus, including a laboratory insecticide susceptible strain (Foshan) and two field deltamethrin-resistant strains (Yuexiu/Baiyun), as well as a laboratory induced deltamethrin-resistant (R18) and its parental insecticide susceptible strains (R0) to characterize their amino acids and acylcarnitines (AcylCNs) profiles by LC-MS/MS spectrometry. Results: Heatmap and PCA analysis of amino acids and AcylCNs profile indicated a clear separation of Foshan from Yuexiu/Baiyun populations, as well as a partly overlapping between Yuexiu and Baiyun populations. All strains were abundant in five amino acids (Pro, Val, Arg, Glu, His) and two AcylCNs (C0, C2). Five amino acids (Tyr, Cit, Pro, Thr, Gly) were significantly higher, while four amino acids (His, Val, Glu, Ala) were obviously lower in Yuexiu strain than Baiyun population. R18 displayed a differential amino acid and AcylCNs profile from its parental R0 strain. Conclusion: This study displayed a distinction of amino acids and AcylCNs profiles between insecticide-resistant and geographically or genetically-close insecticide-susceptible strain of Ae. albopictus, suggesting a possible application of amino acids and AcylCNs profiles for rapid diagnosis of deltamethrin resistance in Ae. albopictus in the future.


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