Genetic variation and population subdivision in Australian barramundi, Lates calcarifer (Bloch)

1985 ◽  
Vol 36 (2) ◽  
pp. 203 ◽  
Author(s):  
JB Shaklee ◽  
JP Salini
Keyword(s):  
Genetic Variation ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Allele Frequencies ◽  
Population Subdivision ◽  
Starch Gel Electrophoresis ◽  
Average Heterozygosity ◽  
Lates Calcarifer ◽  
Repeated Sampling ◽  
Starch Gel

Starch gel electrophoresis of enzymes and polyacrylamide gel electrophoresis of muscle proteins were used to study genetic variation in 589 barramundi from three widely separated regions in northern Australia. Presumed genetic variation was observed at 16 loci (out of 46 screened). However, only 11 of these were polymorphic at the p0.99 level. The average heterozygosity in this species was 0.032. In nearly all cases, genotypic proportions agreed closely with Hardy-Weinberg expectations. Repeated sampling in each region revealed little or no change in allele frequencies over a period of several months. Similarly, comparisons of allele frequencies for fish from marine and from freshwater localities in the south-eastem Gulf of Carpentaria failed to reveal significant genetic differences between habitats. Between-region heterogeneity Χ2 tests indicated substantial genetic differentiation at 10 of the 11I polymorphic loci. These data indicate the existence of at least three distinct stocks or subpopulations of barramundi in Australia.

Survey of the Genetic Variation Among Eastern Lake Superior Lake Trout (Salvelinus namaycush)

1981 ◽  
Vol 38 (12) ◽  
pp. 1738-1746 ◽  
Author(s):  
Terrence R. Dehring ◽  
Anne F. Brown ◽  
Charles H. Daugherty ◽  
Stevan R. Phelps
Keyword(s):  
Genetic Variation ◽  
Gel Electrophoresis ◽  
Lake Trout ◽  
Lake Superior ◽  
Salvelinus Namaycush ◽  
Starch Gel Electrophoresis ◽  
Average Heterozygosity ◽  
Management Implications ◽  
Salmonid Species ◽  
Starch Gel

Patterns of genetic variation among lake trout (Salvelinus namaycush) of eastern Lake Superior were examined using starch gel electrophoresis. We used 484 individuals sampled from three areas, representing three morphological types (leans, humpers, and siscowets). Of 50 loci examined, 44 were monomorphic in all groups sampled. Genetic variation occurs at six loci AAT-1,2, MDH-3,4, ME-1, and SOD-1. The average heterozygosity found (H = 0.015) is low relative to other salmonid species. A significant amount of heterogeneity exists among the 10 lake trout samples. These differences are due to variation within as well as between morphological types. The significance and management implications of these data are discussed.Key words: genetic variation, lake trout, Salvelinus namaycush, Lake Superior

Little genetic variation in the oil sardine, Sardinella longiceps Val., from the western coast of India

1994 ◽  
Vol 45 (2) ◽  
pp. 257 ◽  
Author(s):  
MR Menezes
Keyword(s):  
Genetic Variation ◽  
Gel Electrophoresis ◽  
Western Coast ◽  
Starch Gel Electrophoresis ◽  
Marine Fish Species ◽  
Average Heterozygosity ◽  
Enzyme Gene ◽  
Starch Gel ◽  
Sardinella Longiceps ◽  
Gene Variability

Enzyme gene variability in the oil sardine, Sardinella longiceps, from three localities along the western coast of India was studied by starch-gel electrophoresis. Out of 19 loci scored, no locus was polymorphic by the 95% criterion. Seven loci were polymorphic at the P=0.99 level. The average heterozygosity ranged from 0.6% to 0.9%. These values are very low compared with those of other marine fish species.

Genetic variation in pika populations

1977 ◽  
Vol 55 (11) ◽  
pp. 1841-1845 ◽  
Author(s):  
D. G. Glover ◽  
M. H. Smith ◽  
L. Ames ◽  
J. Joule ◽  
J. M. Dubach
Keyword(s):  
Genetic Variation ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Genetic Loci ◽  
Starch Gel

Analysis of 26 genetic loci by horizontal starch gel electrophoresis was performed on 197 pikas from one Montana and four Colorado populations. Only four loci were polymorphic within populations and individual heterozygosity was low for a mammal [Formula: see text]. The Montana and Colorado populations were fixed for alternate allelles at three loci. Because of the insular nature of pika habitat, drift is probably important in determining low heterozygosities and genetic similarities measured between widely separated pika populations.

The systematic status of the lagoon periwinkle, Littorina tenebrosa

1999 ◽  
Vol 79 (4) ◽  
pp. 653-660 ◽  
Author(s):  
Iain F. Wilson ◽  
Elizabeth M. Gosling ◽  
William Tapper
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Variation ◽  
Gene Flow ◽  
Genetic Distance ◽  
Gel Electrophoresis ◽  
Distinct Group ◽  
Starch Gel Electrophoresis ◽  
Allopatric Populations ◽  
Starch Gel ◽  
The Mean

Eight samples of Littorina tenebrosa and L. saxatilis (Mollusca: Gastropoda) from Ireland and Britain, including pairs of each form from two locations in Ireland, were screened for genetic variation at 12 polymorphic enzyme loci using starch gel electrophoresis. Levels of polymorphism and heterozygosity were similar in L. tenebrosa and L. saxatilis, apart from a sample of L. tenebrosa from Britain which was less polymorphic than the Irish samples. No alleles were found to be unique to either form. Phylogenetic analysis using UPGMA showed that L. saxatilis and L. tenebrosa populations clustered as a monophyletic group. Nevertheless, the mean genetic distance between parapatric populations of L. saxatilis and L. tenebrosa (D=0.076) was similar to the mean for allopatric populations of either species (D=0.080). This indicates that there is a barrier to gene flow between the two forms Despite this, L. tenebrosa does not merit specific status since populations of this snail do not cluster as a distinct group, separate from L. saxatilis populations.

Genotypic Diversity of Kikuyugrass (Pennisetum clandestinum) Populations in California

Weed Science ◽  
1995 ◽  
Vol 43 (2) ◽  
pp. 209-214 ◽  
Author(s):  
Cheryl A. Wilen ◽  
Jodie S. Holt ◽  
Norman C. Ellstrand ◽  
Ruth G. Shaw
Keyword(s):  
Genetic Variation ◽  
Genetic Variability ◽  
Plant Species ◽  
Gel Electrophoresis ◽  
Genotypic Diversity ◽  
Clonal Reproduction ◽  
Golf Course ◽  
Starch Gel Electrophoresis ◽  
Geographic Locations ◽  
Starch Gel

Using starch gel electrophoresis, we examined the genetic variability of kikuyugrass collected from three golf course sites within its geographical range in California. These experiments were conducted to determine the method of spread of kikuyugrass in areas where it is considered invasive. Samples from roughs and fairways of each of these locations were compared. of the 354 plants examined, 12 different genotypes were identified by isozyme analysis, and only three of the nine putative loci varied among the genotypes. Two genotypes, representing 73% of the plants examined, were found at all three geographic locations. Our results imply that under common golf course practices, kikuyugrass is maintained by clonal reproduction. We conclude that low genetic variation does not preclude a plant species from being an aggressive invader.

scholarly journals Electrophoretic and immunological charactorization of rat neurophysin

1971 ◽  
Vol 122 (5) ◽  
pp. 671-676 ◽  
Author(s):  
A. Norström ◽  
J. Sjöstrand ◽  
B. G. Livett ◽  
L. O. Uttenthal ◽  
D. B. Hope
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Minor Component ◽  
Polyacrylamide Gel ◽  
Male Rats ◽  
Major Protein ◽  
Starch Gel Electrophoresis ◽  
Neural Lobe ◽  
Starch Gel

1. The electrophoretic properties of rat posterior pituitary proteins have been compared on starch gel with those of bovine and porcine neurophysins. 2. [35S]-Cysteine was injected into the supraoptic nucleus of male rats and 16–24h later the distribution of labelled neural-lobe protein in starch and polyacrylamide gels was determined. In both systems a single major protein component was found to contain more than 80% of the total recovered radioactivity. Between 5 and 10% of the radioactivity was found in a minor component in polyacrylamide gel. 3. In agar, microimmuno-diffusion and -electrophoresis of the rat neural-lobe proteins gave a single arc with neurophysin antiserum, and after starch-gel electrophoresis this arc was shown to be due to the major labelled component. 4. The molecular weights of the rat neural-lobe proteins were estimated by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. The molecular weight of the major labelled component was found to be 12000. 5. It is concluded that the rat neurophysin consists of one major and possibly one minor component.

Genetic structure of barramundi (Lates calcarifer) stocks from northern Australia

1988 ◽  
Vol 39 (3) ◽  
pp. 317 ◽  
Author(s):  
J Salini ◽  
JB Shaklee
Keyword(s):  
Gel Electrophoresis ◽  
Northern Territory ◽  
Starch Gel Electrophoresis ◽  
Muscle Proteins ◽  
Northern Australia ◽  
Lates Calcarifer ◽  
History Of ◽  
Hardy Weinberg Equilibrium ◽  
Starch Gel ◽  
Management Policies

Barramundi, L. calcarifer, were collected from seven localities in the Northern Territory, the Daly, Finniss, Mary, Glyde, Roper and McArthur rivers and Blue Mud Bay, and from the Ord River in Western Australia. Barramundi were sampled seven times from the Daly and Finniss rivers over a 14-month period. In total, 46 loci were identified using starch-gel electrophoresis of enzymes and polyacrylamide electrophoresis of muscle proteins. Twelve loci were polymorphic at the P0.99 level. Most loci were in Hardy-Weinberg equilibrium. A contingency Χ2 analysis for homogeneity of alleles over all loci and all localities was highly significant (P < 0.001). Comparisons of data from adjacent pairs of localities revealed that the overall heterogeneity was attributable to heterogeneity among seven of the eight localities; the Daly and Finniss river areas were not significantly different from one another. No evidence of heterogeneity over time was found among the collections from the Daly River area. The considerable amount of heterogeneity observed suggests that each of these seven localities supports a genetically discrete stock of barramundi; this conclusion is consistent with the documented life history of Australian barramundi. The genetic heterogeneity of the stocks should be considered when management policies for L. calcarifer are being formulated.

scholarly journals Biochemical variation in purebred and crossbred strains of domestic rabbits (Oryctolagus cuniculus L.)

1986 ◽  
Vol 48 (1) ◽  
pp. 27-34 ◽  
Author(s):  
G. B. Hartl ◽  
H. Höger
Keyword(s):  
Genetic Variation ◽  
Gel Electrophoresis ◽  
Oryctolagus Cuniculus ◽  
Mammalian Species ◽  
Starch Gel Electrophoresis ◽  
Starch Gel ◽  
Biochemical Variation ◽  
Domestic Rabbits ◽  
Different Strains ◽  
Different Tissues

SummaryGenetic variation in 40 domestic rabbits (Oryctolagus cuniculus) from eight different strains was investigated by horizontal starch gel electrophoresis. Twenty nine enzyme systems were examined in different tissues, 10 isoenzymes were found to be polymorphic. Indices of genetic variation show values comparable to those found in most other mammalian species. Thus the unusually high values reported previously by other authors may be due to a limited and not randomly chosen set of enzymes studied.

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