Biosynthesis of Gibberellins in Barley and Dwarf Rice Seedlings

1974 ◽  
Vol 1 (2) ◽  
pp. 199 ◽  
Author(s):  
KF Faull ◽  
BG Coombe ◽  
LG Paleg
Keyword(s):  
Biological Activity ◽  
Liquid Chromatography ◽  
Retention Time ◽  
De Novo ◽  
Mevalonic Acid ◽  
Biologically Active ◽  
Gas Liquid Chromatography ◽  
Rice Seedlings ◽  
Kaurenoic Acid ◽  
Gibberellin A1

Incorporation studies with 14 CO2 suggested that 11-day-old barley seedlings biosynthesized an A1- like gibberellin de novo and that the biosynthesized gibberellin completely turned over within 12 h. Exogenous [14C]mevalonic acid, supplied in a variety of ways, was not incorporated into the gibberellins of barley seedlings. Barley seedlings, which failed to elongate in response to exogenous ent-kaurenoic acid, metabolized exogenous [17-14C]ent-kaurenoic acid to a few compounds, but not to a biologically active gibberellin. Dwarf rice seedlings (cv. Tan-ginbozu), which elongated in response to exogenous ent-kaurenoic acid, metabolized exogenous [17-14C]ent-kaurenoic acid to a number of radioactive compounds, one of which behaved like [14C]gibberellin A1 in that it showed radioactivity and biological activity at the expected retention time during gas-liquid chromatography.

Measurement of carbamazepine and its epoxide metabolite by high-performance liquid chromatography, and a comparison of assay techniques for the analysis of carbamazepine.

1977 ◽  
Vol 23 (12) ◽  
pp. 2283-2287 ◽  
Author(s):  
G W Mihaly ◽  
J A Phillips ◽  
W J Louis ◽  
F J Vajda
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Plasma Concentrations ◽  
Correlation Coefficients ◽  
High Performance Liquid Chromatographic ◽  
Biologically Active ◽  
Gas Liquid Chromatography ◽  
Carbamazepine Therapy ◽  
Regression Slopes

Abstract We describe a modified high-performance liquid-chromatographic method for the simultaneous analysis of carbamazepine andits biologically active metabolite, carbamazepine-10, 11-epoxide. Concentrations of both these compounds in the plasma of 35 epileptic patients receiving chronic carbamazepine therapy are presented. Concentrations of carbamazepine in plasma were related to those of carbamazepine-10, 11-epoxide (r - 0.495, P less than 0.05). Total daily doses of carbamazepine were better correlated with plasma concentrations of carbamazepine-10, 11-epoxide (r = 0.714, P less than 0.001) than of carbamazepine (r = 0.269, P greater than 0.05). Close correlations were found between results of the three assay procedures we used to measure plasma carbamazepine concentrations: high-performance liquid chromatography, gas-liquid chromatography, and enzyme immunoassay. Correlation coefficients exceeded 0.97 and regression slopes were near unity, indicating that all three procedures were individually specific for the quantification of plasma carbamazepine.

Classification of some dermatophytes by pyrolysis–gas–liquid chromatography

1973 ◽  
Vol 19 (4) ◽  
pp. 403-407 ◽  
Author(s):  
J. W. Carmichael ◽  
Awatar S. Sekhon ◽  
Lynne Sigler
Keyword(s):  
Liquid Chromatography ◽  
Mating Type ◽  
Retention Time ◽  
Gas Liquid Chromatography ◽  
Mating Types ◽  
Random Drift ◽  
Characteristic Peak ◽  
Pyrolysis Gas ◽  
Arthroderma Benhamiae

Samples from dried colonies of 21 strains of Nannizzia and Arthroderma were analyzed by pyrolysis–gas–liquid chromatography. Characteristic peak patterns produced by all the strains were used as markers to correct random drift in retention time so that corresponding peaks in different pyrograms could be homologized. Variation in sample size was compensated for by comparing peaks on each pyrogram with a particular major component and scoring them simply as 0 (absent), 1 (small), or 2 (large). Proximities were calculated and analyzed for clusters by the TAXMAP procedure. The analysis always grouped replicate samples together in the same cluster. Opposite mating types of the same species were sometimes placed in the same cluster and sometimes in separate clusters. The (+) mating type of Arthroderma benhamiae was placed in a cluster with both mating types of Nannizzia gypsea and N. obtusa, while the (−) mating type replicates of A. benhamiae were placed in a cluster by themselves. Finding a greater difference between pyrograms of different mating types of one species than between pyrograms of different species was unexpected and requires further investigation.

STUDIES ON THE DISSIPATION OF DIFLUBENZURON AND METHOPRENE FROM SHALLOW PRAIRIE POOLS

1980 ◽  
Vol 112 (2) ◽  
pp. 173-177 ◽  
Author(s):  
D. J. Madder ◽  
W. L. Lockhart
Keyword(s):  
Biological Activity ◽  
Liquid Chromatography ◽  
Growth Regulator ◽  
Mosquito Control ◽  
Parent Compound ◽  
Gas Liquid Chromatography ◽  
Mosquito Larvae ◽  
Canadian Prairie ◽  
Repeated Applications

AbstractA series of sod-lined pools were constructed and used to monitor repeated applications of diflubenzuron as Dimilin WP-25 and mefhoprene as Altosid SR-10. Diflubenzuron and methoprene "disappeared" rapidly from the pool water as determined by bioassays using Aedes aegypti (L.) (Culicidae) larvae and by gas-liquid chromatography (GLC). Chemical assays for a diflubenzuron derivative were positive for several days; bioassay indicated the presence of diflubenzuron (or at least growth regulator activity) at levels toxic to mosquito larvae for up to 16 days. In contrast methoprene "fell" below GLC detection within 2 days although biological activity persisted for approximately a week after treatment. Neither parent compound should cause a long-term persistence hazard when used for mosquito control in Canadian prairie waters.

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