Retention Time in Nonlinear Gas-Liquid Chromatography. Influence of the Sample Size

1970 ◽  
Vol 5 (5) ◽  
pp. 545-554 ◽  
Author(s):  
Jean-Yves Lenoir ◽  
Alexandre Rojey
Keyword(s):  
Liquid Chromatography ◽  
Sample Size ◽  
Retention Time ◽  
Gas Liquid Chromatography

Classification of some dermatophytes by pyrolysis–gas–liquid chromatography

1973 ◽  
Vol 19 (4) ◽  
pp. 403-407 ◽  
Author(s):  
J. W. Carmichael ◽  
Awatar S. Sekhon ◽  
Lynne Sigler
Keyword(s):  
Liquid Chromatography ◽  
Mating Type ◽  
Retention Time ◽  
Gas Liquid Chromatography ◽  
Mating Types ◽  
Random Drift ◽  
Characteristic Peak ◽  
Pyrolysis Gas ◽  
Arthroderma Benhamiae

Samples from dried colonies of 21 strains of Nannizzia and Arthroderma were analyzed by pyrolysis–gas–liquid chromatography. Characteristic peak patterns produced by all the strains were used as markers to correct random drift in retention time so that corresponding peaks in different pyrograms could be homologized. Variation in sample size was compensated for by comparing peaks on each pyrogram with a particular major component and scoring them simply as 0 (absent), 1 (small), or 2 (large). Proximities were calculated and analyzed for clusters by the TAXMAP procedure. The analysis always grouped replicate samples together in the same cluster. Opposite mating types of the same species were sometimes placed in the same cluster and sometimes in separate clusters. The (+) mating type of Arthroderma benhamiae was placed in a cluster with both mating types of Nannizzia gypsea and N. obtusa, while the (−) mating type replicates of A. benhamiae were placed in a cluster by themselves. Finding a greater difference between pyrograms of different mating types of one species than between pyrograms of different species was unexpected and requires further investigation.

Pyrolysis–gas–liquid chromatography of some dermatophytes

1972 ◽  
Vol 18 (10) ◽  
pp. 1593-1601 ◽  
Author(s):  
Awatar S. Sekhon ◽  
J. W. Carmichael
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Sample Size ◽  
Gas Liquid Chromatography ◽  
Mating Types ◽  
Pyrolysis Gas

Dermatophytes belonging to Nannizzia, Arthroderma, and Microsporum were examined by pyrolysis–gas–liquid chromatography. Replicate samples from the same colony, or samples from another colony of the same strain, yielded closely similar pyrograms, but the curves were not quite superimposable. Effects on the pyrolysis patterns of sample size, age of colony, and type of medium were investigated. Pyrograms of the plus and minus mating types of the species of Nannizzia and Arthroderma were similar to each other, but they did differ in some respects. The patterns of Microsporum audouini and M. canis differed considerably from each other. Quantitative analysis of the pyrochromatographic data, using several procedures, did not yield satisfactory results. It was found that variation between replicates was often as great as between species.

Biosynthesis of Gibberellins in Barley and Dwarf Rice Seedlings

1974 ◽  
Vol 1 (2) ◽  
pp. 199 ◽  
Author(s):  
KF Faull ◽  
BG Coombe ◽  
LG Paleg
Keyword(s):  
Biological Activity ◽  
Liquid Chromatography ◽  
Retention Time ◽  
De Novo ◽  
Mevalonic Acid ◽  
Biologically Active ◽  
Gas Liquid Chromatography ◽  
Rice Seedlings ◽  
Kaurenoic Acid ◽  
Gibberellin A1

Incorporation studies with 14 CO2 suggested that 11-day-old barley seedlings biosynthesized an A1- like gibberellin de novo and that the biosynthesized gibberellin completely turned over within 12 h. Exogenous [14C]mevalonic acid, supplied in a variety of ways, was not incorporated into the gibberellins of barley seedlings. Barley seedlings, which failed to elongate in response to exogenous ent-kaurenoic acid, metabolized exogenous [17-14C]ent-kaurenoic acid to a few compounds, but not to a biologically active gibberellin. Dwarf rice seedlings (cv. Tan-ginbozu), which elongated in response to exogenous ent-kaurenoic acid, metabolized exogenous [17-14C]ent-kaurenoic acid to a number of radioactive compounds, one of which behaved like [14C]gibberellin A1 in that it showed radioactivity and biological activity at the expected retention time during gas-liquid chromatography.

The Effect of Stereoisomeric Structure on Retention Time in Gas Liquid Chromatography of Per-O-trimethylsilyl Derivatives of Pentoses and Hexoses

1971 ◽  
Vol 26 (1) ◽  
pp. 24-25 ◽  
Author(s):  
Th. Gheorghiu ◽  
K. Oette
Keyword(s):  
Liquid Chromatography ◽  
Retention Time ◽  
Conformational Stability ◽  
Gas Liquid Chromatography ◽  
Oh Groups ◽  
Ring Form ◽  
Anomeric Carbon ◽  
Trimethylsilyl Derivatives ◽  
Derivatives Of

The analysis of relationships between structure and retention time in gas liquid chromatography of pentoses and hexoses leads to the formulation of following rule: the per-O-trimethylsilyl derivatives of aldoses stereoisomers with conformational stability, identical ring form and the same number of substituted OH groups are longer retained on polar columns when the equatorial substituents are more numerous and are located nearer to the anomeric carbon.

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