Immune response of the tammar wallaby (Macropus eugenii) to sperm antigens

2003 ◽  
Vol 15 (8) ◽  
pp. 429 ◽  
Author(s):  
D. J. Kay ◽  
A. L. Kitchener
Keyword(s):  
Immune Response ◽  
Follicular Fluid ◽  
Surface Antigens ◽  
Tammar Wallaby ◽  
Male And Female ◽  
Macropus Eugenii ◽  
Membrane Extract ◽  
Sperm Antigens ◽  
Live Sperm ◽  
Sperm Antibodies

In the present study, male and female tammar wallabies were immunised with whole tammar wallaby sperm in adjuvant. An assay for sperm antibodies using a live sperm ELISA has been developed to detect sperm surface antigens and used to validate an assay using a 3-[(3-cholamidopropyl) dimethylammonio]-1 propanesulfonate (CHAPS) membrane extract of whole tammar wallaby sperm. The tests were used to monitor the immune response to whole sperm in both male and female tammar wallabies. Antisera with a limited array of specificities were generated, with those locating to the midpiece region of the sperm appearing the most likely candidates for targets for fertility perturbation based on immunofluorescence of fixed and non-fixed sperm. These systemically generated antibodies were demonstrated to have access to both the female and male tammar reproductive tracts and were found on ejaculated sperm and antibodies from female sera and follicular fluid-labelled fresh ejaculated sperm from non-immunised males. Preliminary sequencing of these proteins has identified some possibilities for further investigation.

scholarly journals Identification of Arvicola terrestris scherman Sperm Antigens for Immune Contraceptive Purposes

2021 ◽  
Vol 22 (18) ◽  
pp. 9965
Author(s):  
Areski Chorfa ◽  
Chantal Goubely ◽  
Joelle Henry-Berger ◽  
Rachel Guiton ◽  
Joël R. Drevet ◽  
...  
Keyword(s):  
Immune Response ◽  
Vaccination Strategy ◽  
Male Gamete ◽  
Sperm Cells ◽  
Reproductive Process ◽  
Male And Female ◽  
Arvicola Terrestris ◽  
Contraceptive Vaccine ◽  
Sperm Antigens ◽  
Immunogenic Proteins

The cyclical proliferation of the wild fossorial rodent Arvicola terrestris scherman (ATS) is critical in mid-mountain ecosystems of several European countries. Our goal is to develop an immunocontraceptive vaccine to control their fertility, as a sustainable alternative to chemical poisons currently used. Indeed, these chemicals cause the death of ATS predators and animals sharing their ecosystem, and current laws progressively limit their use, making the development of a targeted vaccination strategy an interesting and efficient alternative. In order to identify species-specific sperm antigens, male and female ATS received subcutaneous injections of whole ATS spermatozoa to elicit an immune response. The analysis of the immune sera led to the identification of 120 immunogenic proteins of sperm cells. Of these, 15 were strictly sperm-specific and located in different regions of the male gamete. Some of these antigens are proteins involved in molecular events essential to the reproductive process, such as sperm–egg interaction, acrosomal reaction, or sperm motility. This approach not only identified a panel of immunogenic proteins from ATS sperm cells, but also demonstrated that some of these proteins trigger an immune response in both male and female ATS. These spermatic antigens are good candidates for the development of a contraceptive vaccine.

Temperature gradients in vivo influence maturing male and female gametes in mammals: evidence from the cow

2017 ◽  
Vol 29 (12) ◽  
pp. 2301 ◽  
Author(s):  
R. H. F. Hunter ◽  
F. López-Gatius ◽  
O. López-Albors
Keyword(s):  
Follicular Fluid ◽  
Open Surgery ◽  
Temperature Gradients ◽  
Fluid Temperature ◽  
Preovulatory Follicle ◽  
Male And Female ◽  
Direct Measurements ◽  
Final Maturation ◽  
Preovulatory Follicles

Since 1980 several reports have indicated that temperatures vary between preovulatory follicles and other ovarian tissues in rabbit, cow, pig and human. However, these observations did not achieve prominence; they were regarded as artefacts due to the use of anaesthetics and open surgery (laparotomy). Recently, without resorting to anaesthesia or surgery, direct measurements of temperature in preovulatory follicles have been performed in the cow by means of a thermistor probe introduced into the antrum under ultrasonic guidance. Such follicles revealed a mean antral (follicular fluid) temperature 0.74°C and 1.54°C cooler than uterine surface and rectal temperatures respectively in ovulating cows, whereas no such temperature differences were detected in non-ovulating cows. Cows are predominantly monovular and preovulatory follicles attain a diameter of 15–22 mm or more. These features and the timescale of response to the preovulatory gonadotrophin surge make them a valuable model for the human preovulatory follicle. Temperature gradients are interpreted primarily in a context of final maturation of gametes immediately before the onset of fertilisation. Preovulatory follicular temperature in women could be assessed by a comparable approach and might become a valuable selection guide for oocyte viability.

scholarly journals Growth of the corpus luteum and its progesterone content during pregnancy in the tammar wallaby, Macropus eugenii

Reproduction ◽  
1979 ◽  
Vol 57 (1) ◽  
pp. 131-136 ◽  
Author(s):  
M. B. Renfree ◽  
S. W. Green ◽  
I. R. Young
Keyword(s):  
Corpus Luteum ◽  
Tammar Wallaby ◽  
Macropus Eugenii
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