Ovariectomy increases the phenotypic plasticity of the female prostate epithelium in the Mongolian gerbil (Meriones unguiculatus)

2017 ◽  
Vol 29 (9) ◽  
pp. 1751 ◽  
Author(s):  
Marianna Zanatelli ◽  
Ana M. G. Custodio ◽  
Manoel F. Biancardi ◽  
Fernanda C. A. Santos ◽  
Rejane M. Góes ◽  
...  
Keyword(s):  
Periodic Acid ◽  
Meriones Unguiculatus ◽  
Neuroendocrine Cells ◽  
Cellular Heterogeneity ◽  
Secretory Cells ◽  
Mongolian Gerbils ◽  
Sexual Hormones ◽  
Periodic Acid Schiff ◽  
Female Prostate ◽  
Prostate Epithelium

The female prostate is a reproductive gland that typically presents a morphology similar to that of the male gland and is highly developed in female Mongolian gerbils. Two main cell populations compose the epithelium gland: basal and secretory luminal cells. However, during postnatal development, diverse secretory cell phenotypes are distributed among the typical ones. Prostate homeostasis is under the control of sexual hormones, such as oestrogen and progesterone. After hormonal deprivation the female gland undergoes several morphophysiological changes. The objective of this study was to identify and characterise, structurally and ultrastructurally, the cellular heterogeneity of the female prostate epithelium in normal conditions and after ovariectomy. Histological routine stains, such as haematoxylin–eosin, periodic acid–Schiff and silver impregnation, as well as immunocytochemical techniques were used to enable identification of the different cell types. Some secretory cells types were identified and characterised as mucinous, basophil, clear, ciliated, droplet, spumous and neuroendocrine cells. Population tally data showed that the hormonal suppression caused by ovariectomy resulted in a decrease in the proportions of basophil and clear cells and an increase in spumous cells. Thus, the secretory epithelial cells of the female gerbil prostate are not morphologically and functionally uniform, presenting a phenotypical plasticity according to the hormonal environment in which they operate.

scholarly journals MORPHOLOGICAL AND CYTOCHEMICAL PROPERTIES OF THE HOLOCRINE CELLS IN THE EPIDIDYMIS OF THE MOUSE

1964 ◽  
Vol 12 (8) ◽  
pp. 628-639 ◽  
Author(s):  
JAN MARTAN ◽  
JOHN M. ALLEN
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Periodic Acid ◽  
Succinic Dehydrogenase ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Central Nucleus ◽  
Nicotinamide Adenine ◽  
Secretory Cells ◽  
Basal Cells ◽  
Periodic Acid Schiff ◽  
Highly Active

Holocrine secretory cells have been identified in the epithelium of the epididymal canal of the mouse. These cells develop from basal cells. During their differentiation they grow toward the lumen of the epididymal canal and come to form club-shaped structures with an expanded apical portion, a central nucleus and a thin stalk-like connection to the basement membrane. Mature holocrine cells are characterized by their high acid phosphatase and aliesterase activity. They also are highly active for succinic dehydrogenase, nicotinamide adenine dinucleotide diaphorase, and nicotinamide adenine dinucleotide phosphate diaphorase. Nucleoside diphosphatase, thiamine pyrophosphatase, adenosine triphosphatase, and alkaline nucleoside phosphatase are also found in these cells. These cells are also characterized by their reactivity with the Aoyama and periodic acid-Schiff reactions. They react moderately with the molybdate and Luxol Fast Blue MBS reactions for choline containing compounds. Mature holocrine cells may disintegrate in situ or may be discharged in toto into the lumen of the epididymal canal. Glycerylphosphorylcholine was identified in extracts prepared from sperm-free epididymides of mice. Glycerylphosphorylcholine reacts with Aoyama and periodic acid-Schiff reactions as do mature holocrine cells. This fact coupled with the identification of choline containing material in holocrine cells suggests that they may be one site for the formation of glycerylphosphorylcholine.

Agarose plug instillation causes goblet cell metaplasia by activating EGF receptors in rat airways

2000 ◽  
Vol 278 (1) ◽  
pp. L185-L192 ◽  
Author(s):  
Heung-Man Lee ◽  
Kiyoshi Takeyama ◽  
Karim Dabbagh ◽  
James A. Lausier ◽  
Iris F. Ueki ◽  
...  
Keyword(s):  
Alcian Blue ◽  
Goblet Cell ◽  
Kinase Inhibitor ◽  
Periodic Acid ◽  
Goblet Cells ◽  
Secretory Cells ◽  
Egf Receptors ◽  
Periodic Acid Schiff ◽  
Egfr Expression ◽  
Goblet Cell Metaplasia

We hypothesized that foreign bodies in airways cause inflammation leading to goblet cell metaplasia. Instilled agarose plugs lodged in the bronchi of pathogen-free rats caused a time-dependent increase in Alcian blue-periodic acid-Schiff staining that was detected within 24 h and markedly increased at 72 h. Control bronchi contained no pregoblet or goblet cells, but plugged bronchi contained many pregoblet and goblet cells and a decrease in nongranulated secretory cells. In situ hybridization showed no expression of MUC5AC in control airways, but plugged airways showed a marked expression. Control bronchi showed sparse staining for epidermal growth factor receptor (EGFR) protein, but plugged bronchi showed intense EGFR staining in the epithelium. Pretreatment with an EGFR tyrosine kinase inhibitor (BIBX1522) prevented Alcian blue-periodic acid-Schiff staining and MUC5AC gene expression in plugged bronchi. Pretreatment with tumor necrosis factor-α neutralizing antibody or pretreatment with cyclophosphamide abolished plug-induced EGFR protein expression and goblet cell metaplasia. Thus instillation of agarose plugs induces profound goblet cell metaplasia by causing EGFR expression and activation.

THE DIGESTIVE TRACT OF HERMODICE CARUNCULATA (PALLAS). POLYCHAETA: AMPHINOMIDAE

1963 ◽  
Vol 41 (2) ◽  
pp. 165-184 ◽  
Author(s):  
Joan Rattenbury Marsden
Keyword(s):  
Digestive Tract ◽  
Buccal Cavity ◽  
Periodic Acid ◽  
The Body ◽  
Gut Contents ◽  
Secretory Cells ◽  
Stomatogastric Nervous System ◽  
Foreign Inclusion ◽  
Periodic Acid Schiff ◽  
Hermodice Carunculata

Hermodice carunculata is common among the coral reefs and shallow waters of the West Indies where it has been observed to feed on living coral. Examination of gut contents reveals that this species is probably omnivorous. The digestive tract consists of five regions, a buccal cavity which is eversible, a muscular pharynx, a short oesophagus, an intestine which can be separated histologically into anterior and posterior regions, and a rectum. Elaborations of the buccal epithelium form muscular and glandular areas. Secretory materials are formed in considerable quantity by the glandular portion of the buccal cavity and by the anterior intestine. Secretory cells present in lesser abundance in other areas are described. Amoebocytes laden with foreign particles may accumulate in the wall of the rectum. Other foreign inclusion masses may be found in the epithelia of the buccal cavity, pharynx, and oesophagus as well as in the mid-ventral line of the body wall. The various parts of the digestive tract were tested for Y metachromasia with azure A and were treated with the periodic acid Schiff technique. The stomatogastric nervous system was followed.

scholarly journals Histological Observation on the Female Prostate in Mongolian Gerbils (Meriones unguiculatus)

1992 ◽  
Vol 41 (3) ◽  
pp. 383-386
Author(s):  
Tomoo INOMATA ◽  
Hiroyoshi NINOMIYA ◽  
Hiroaki SOMIYA ◽  
Hiroshi SAITO ◽  
Koshi MOCHIZUKI
Keyword(s):  
Meriones Unguiculatus ◽  
Mongolian Gerbils ◽  
Histological Observation ◽  
Female Prostate

scholarly journals Structural and Ultrastructural Morphological Evaluation of Giant Anteater (Myrmecophaga tridactyla) Prostate Gland

Biology ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 231
Author(s):  
Fernanda Moura ◽  
Letícia Sampaio ◽  
Priscila Kobayashi ◽  
Renee Laufer-Amorim ◽  
João Carlos Ferreira ◽  
...  
Keyword(s):  
Periodic Acid ◽  
Prostate Gland ◽  
Specific Antigen ◽  
Species Conservation ◽  
Ultrastructural Feature ◽  
Secretory Cells ◽  
Morphological Description ◽  
Basal Cells ◽  
Periodic Acid Schiff ◽  
Young Subjects

The giant anteater (Myrmecophaga tridactyla) is a vulnerable species from Central and South America, and is considered possibly extinct in Belize, Guatemala, El Salvador, and Uruguay. Due to the species’ conservation and reproductive importance, this research aimed to characterize the morphology, histochemical, immunohistochemical, and ultrastructural feature of the giant anteater prostate gland. For this, we collected 11 giant anteater prostate glands and performed macroscopic, morphological, histochemical, immunohistochemical, and ultrastructural analysis. Nine prostate glands from an adult subject and two from young subjects were studied. Grossly, the adult giant anteater prostate gland is divided in two distinct zones; the central zones (composed mainly of ducts) and the peripheral zones (of acini formed by secretory cells). The secretory cells showed positive periodic acid–Schiff staining. Furthermore, the immunohistochemical characterization revealed a similar human prostate pattern, with p63 staining basal cells, uroplakin III (UPIII) superficial cells of prostatic urethra, androgen receptor (AR) expressing nucleus of secretory and stromal cells, and prostatic specific antigen (PSA) staining prostatic epithelial cells. Overall, our research provided an in-depth morphological description of the giant anteater’s prostate gland, providing valuable information for futures studies focused on giant anteater conservation.

Growth and differentiation of tracheal epithelial progenitor cells

1994 ◽  
Vol 266 (3) ◽  
pp. L296-L307 ◽  
Author(s):  
J. Y. Liu ◽  
P. Nettesheim ◽  
S. H. Randell
Keyword(s):  
Monoclonal Antibody ◽  
Periodic Acid ◽  
Ciliated Cell ◽  
Secretory Cells ◽  
Parent Cell ◽  
Ciliated Cells ◽  
Periodic Acid Schiff ◽  
Epithelial Regeneration ◽  
Poorly Differentiated ◽  
Tracheal Epithelial

The purpose of these studies was to determine whether both basal and secretory rat tracheal epithelial (RTE) cells served as multipotent epithelial progenitors and whether both cell types gave rise to a similar "poorly differentiated" cell during the early phase of epithelial regeneration in denuded tracheal grafts. Griffonia simplicifolia I (GSI) lectin and flow cytometry were used for cell sorting. More than 98% of GSI-positive cells expressed plasma membrane alpha 1-3 terminal galactose (Gal), and 95% contained keratin 14 (K14), phenotypic markers for basal cells; < 1% were secretory or ciliated cells. Less than 2% of the GSI-negative cells expressed Gal or K14, but this fraction contained 16% ciliated cells and 54-79% secretory cells, dependent on whether periodic acid-Schiff staining or binding of an anti-secretory cell monoclonal antibody (RTE 12) was used as the criterion. Equal numbers of viable cells from either fraction were inoculated into denuded tracheal grafts, which were studied on days 1-14. At 24 h, greater numbers of GSI-negative than -positive cells were found attached to the graft wall; the keratin staining pattern of the attached cells was similar to that of the parent cell populations, but monoclonal antibody-detectable secretory and ciliated cell epitopes, originally present in the GSI-negative fraction, were lost. 5-Bromo-2'-deoxyuridine uptake was not seen at 24 h, but by 48 h all epithelial cells from both fractions entered the cell cycle. From 48 to 96 h, cells derived from either fraction were ultrastructurally indistinguishable; they were poorly differentiated and highly proliferative, and all expressed Gal and K14. A mature epithelium evolved from the poorly differentiated cells in both sets of grafts, but secretory and ciliated cells appeared earlier in grafts inoculated with GSI-negative cells. The results strongly suggest that in this model of tracheal epithelial regeneration both basal and secretory cells "dedifferentiated" into a similar highly proliferative phenotype from which a mucociliary epithelium "redifferentiated."

scholarly journals THE ORAL SUCKER OF SOME GASTROINTESTINAL TREMATODES: HISTOLOGICAL AND HISTOCHEMICAL RESEARCH

2021 ◽  
pp. 317-322
Author(s):  
Manikovskaya ◽  
Nacheva
Keyword(s):  
Oral Sucker ◽  
Periodic Acid ◽  
Neurosecretory Cells ◽  
Bromophenol Blue ◽  
Secretory Cells ◽  
Periodic Acid Schiff ◽  
Intense Staining ◽  
Waste Products ◽  
Muscle Complex ◽  
Schiff Reaction

We conducted histological and histochemical studies of the oral sucker of gastrointestinal trematodes of the family Paramphistomatidae, Fischoeder, 1901 living in the rumen of ruminants, namely, Liorchis scotiae, Paramphistomum cervi and Paramphistomum ichikawai. The oral sucker of paramphistomes and the Liorchis scotiae trematode is a complex muscular organ modified into a pharynx-sucker, the muscle complex of which is formed from longitudinal, circular and radial muscle fibers. In the thick pharynx wall, we found secretory cells, single neurosecretory cells and many desmoblastic cells of connective tissue. Histochemical stains showed intense staining with alcian blue, toluidine blue, bromophenol blue and a positive periodic acid Schiff reaction, which indicates the presence of glycosaminoglycans, total proteins and glycoproteins. Due to this structure, the pharynx sucker of gastrointestinal trematodes is involved in capturing food and evacuating its undigested residues from the helminth's body to the outside. In addition, the substances produced by secretory cells of the pharynx carry out a partial chemical treatment of the food consumed by the parasite and protect the parasite from substances that are metabolites of the host tissues and waste products of symbionts and commensals that inhabit the rumen of ruminants. Thus, trophic adaptation of the parasite in its ecological niche, the rumen of polygastric mammals, is ensured.

Proximal airway mucous cells of ovalbumin-sensitized and -challenged Brown Norway rats accumulate the neuropeptide calcitonin gene-related peptide

2004 ◽  
Vol 287 (2) ◽  
pp. L286-L295 ◽  
Author(s):  
Shawnessy D. Larson ◽  
Charles G. Plopper ◽  
Greg Baker ◽  
Brian K. Tarkington ◽  
Kendra C. Decile ◽  
...  
Keyword(s):  
Periodic Acid ◽  
Mucous Cell ◽  
Calcitonin Gene Related Peptide ◽  
Neuroendocrine Cells ◽  
Brown Norway ◽  
Mucous Cells ◽  
Periodic Acid Schiff ◽  
Related Peptide ◽  
Afferent Nerves ◽  
Calcitonin Gene

Mucous cell hypersecretion and increased neuropeptide production play a role in the exacerbation of symptoms associated with asthma. The source of these neuropeptides have been confined to the contributions of small afferent nerves or possibly neuroendocrine cells. We tested the hypothesis that repeated exposure to allergen would alter the sources and abundance of neuropeptides in airways. Right middle lobes from rats (8 wk old) exposed to 2.5% ovalbumin (OVA) for five episodes (30 min each) or filtered air were inflation fixed with paraformaldehyde. The lobes were dissected to expose the airway tree, permeabilized with DMSO, and incubated in antibody to rat calcitonin gene-related peptide (CGRP), followed with a fluorochrome-labeled second antibody. CGRP-positive structures were imaged via confocal microscopy. Airways were later embedded in plastic and sectioned for cell identification. In animals challenged with OVA, CGRP-positive cells, not neuroendocrine or neuronal in origin (confirmed by a lack of protein gene product 9.5 signal), were recorded along the axial path. In section, this fluorescent signal was localized to granules within epithelial cells. Alcian blue/periodic acid-Schiff staining of these same sections positively identify these cells as mucous cells. Mucous cells of animals not challenged with OVA were not positive for CGRP. We conclude that episodic allergen exposure results in the accumulation of CGRP within mucous cells, creating a new source for the release of this neuropeptide within the airway.

Prenatal and pubertal exposure to 17α-ethinylestradiol disrupts folliculogenesis and promotes morphophysiological changes in ovaries of old gerbils (Meriones unguiculatus)

2021 ◽  
pp. 1-12
Author(s):  
Vinícius Gonçalves de Souza ◽  
Laura Borges Bandeira ◽  
Nátaly Caroline Silva e Souza ◽  
Sebastião Roberto Taboga ◽  
Tracy Martina Marques Martins ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Morphological Changes ◽  
Tunica Albuginea ◽  
Periodic Acid ◽  
Primordial Follicle ◽  
Meriones Unguiculatus ◽  
Periodic Acid Schiff ◽  
Endocrine Disrupting Chemical ◽  
Environment Exposure ◽  
Endocrine Organs

Abstract 17α-Ethinylestradiol is an endocrine-disrupting chemical that make up most contraceptive pills and can be found in the environment. Exposure to ethinylestradiol in different development periods may promote changes in morphophysiological parameters of reproductive and endocrine organs. Considering that the effects of low doses (15 µg/kg/day) of ethinylestradiol in ovaries from 12-month-old female gerbils (Meriones unguiculatus) were investigated. Four experimental groups used were control (without treatment), EE/PRE (treated from the 18th to the 22nd gestational day), EE/PUB (treated from the 42nd to the 49th day of life), and EE/PRE-PUB (treated in the both periods). The animals were euthanized at 12 months. Testosterone and 17β-estradiol levels were measured. The ovaries were stained with Hematoxylin and Eosin, Periodic Acid Schiff, and Gomori’s Trichome. The follicles, corpus luteum, interstitial gland, lipofuscin, ovarian epithelium, and tunica albuginea were analyzed. Estradiol was higher in EE/PRE and EE/PUB groups, while testosterone was higher only in EE/PUB group. The main changes in follicle count occurred in EE/PUB and EE/PRE-PUB groups, with higher primordial follicle count and lower maturation of follicles. The corpus luteum was more evident in EE/PRE group. No differences were found in atretic follicles count. A higher area occupied by interstitial gland cells and lipofuscin deposit in these cells was noted in EE/PUB and EE/PRE-PUB groups. Higher epithelium height and thicker tunic albuginea were showed in treated groups. These results suggest that exposure to doses of EE2 in prenatal and pubertal periods of the development leads to morphological changes in senile ovaries.

scholarly journals 3. MORFOLOGI DAN KANDUNGAN KARBOHIDRAT KELENJAR LINGUALIS DAN SUBLINGUALIS ITIK (Anas plathyrinchos) (Morphology and Carbohydrates Content in Lingual and Sublingual Glands of Duck (Anas plathyrinchos))

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
M Jalaluddin
Keyword(s):  
Secretory Cell ◽  
Periodic Acid ◽  
Secretory Cells ◽  
Sublingual Gland ◽  
Epithelial Layer ◽  
Periodic Acid Schiff ◽  
Tubular Gland ◽  
One Year ◽  
Hematoxylin Eosin ◽  
Carbohydrate Contents

This study was aimed to find out the morphology and carbohydrates content of lingual and sub-lingual glands of duck (Anas plathyrinchos). Sample used in this study was lingual and sublingual glands of a one year old female duck. Anatomy morphology and histology of lingual and sublingual glands were observed after processed into histological preparation and stained with hematoxylin-eosin (HE) then stained further with alcian blue (AB) pH 2.5 and periodic acid Schiff (PAS) to detect carbohydrate contents. The results showed that the lingual gland of duck cannot be observed due to its location below the epithelial layer of the tongue. While the sublingual gland of duck has an elongated shape and not granulated like typically glands, located at ventral tongue (dorsoventral lingual) between os mandibulae and covered with musculus digastricus venter oral. Staining with AB pH 2.5 and PAS showed that cytoplasm of secretory cell and secrete of lingual and sublingual glands contain acidic and neutral carbohydrates which indicated a positive reaction with intensity ranging from weak (+) to strong (+++). This study concluded that the lingual and sublingual gland found in female duck is a complex tubular gland with mucous secretory cells type. ____________________________________________________________________________________________________________________

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