Design and validation of a tool to assess the safety principles of working with nanomaterials in nanotechnology laboratories

Introduction: The unique properties of nanomaterials, in addition to their applications in science and technology, can be a threat to human health and the environment. Exposure to these materials may occur in workplaces or research laboratories. It can be said that about half of people with occupational exposure to nanomaterials work in academic environments and laboratories. So, the present study was conducted to design and validate a tool to investigate the principles of working safely with nanomaterials in research laboratories. Materials & Methods: The tool was designed using reports, instructions and articles related to " Methods of working safely with nanomaterials". The validity of the tool was assessed using Lawshe method by calculating CVI and CVR. The reliability of the instrument was evaluated using Cronbach's alpha coefficient and kappa coefficient. Results: The tool was designed in 5 sections: "General Information", "Transportation and Storage of Nanomaterials", "Engineering Controls", "Administrative Controls" and "Personal Protection Equipment". After validation, 5 items did not get the necessary points to stay in the tool and were removed. The Cronbach's alpha value for each section of the tool was more than 0.80, indicating that the was "appropriate". Conclusion: According to the results, it seems that the tool studied in this study is compatible for the designed purpose and is ready to be used as a questionnaire or checklist.

New Nutritional Perspectives in the Context of Chronic Disease Patient Management

As living standards change with the development of modern industry and social encounters, people tend to change their lifestyle and environment exposure along with their psychophysiological factors, leading to an imbalance of homeostasis and increasing the risk for chronic diseases. In addition to ingredients, methods, and food conditions storage and processing, the use of additives and certain new foods have facilitated the increased occurrence of chronic diseases in children or adults. The interaction of some components of the food system with enzymes that metabolize different types of drugs can affect the body's clearance and therapeutic index.The objective of this chapter was to present the general principles of food development for special nutritional conditions, also the adjuvants used for chronic disease status improvement, under the condition of nutritional nutrivigilence and food safety standards, and specific to introduce an adjuvant food for atopic dermatitis management.

Super-sparse marine 3D: A game changer for seismic exploration

TotalEnergies SE and partners Shell and PetroSA recently completed the acquisition and processing of a large (10,000 km2) ultra-sparse (200 m between streamers) marine seismic acquisition survey off the west coast of South Africa in block 5/6/7 using a large PGS Titan Class Ramform vessel. The sparse design enabled fast acquisition and low survey cost and health, safety, and environment exposure. Advances in sparse processing enabled high-quality final seismic data consistent with the exploration objectives. In addition, DUG optimized the 4D regularization/interpolation parameters to approach the near offsets differently than the offsets with more complete coverage to help several processing steps. The survey was designed to be upgradable to a higher-resolution survey if needed via the addition of a custom regular infill pattern, either in its entirety or over targeted areas.

New Discoveries and Ambiguities of Nrf2 and ATF3 Signaling in Environmental Arsenic-Induced Carcinogenesis

Environment exposure to arsenic had been linked to increased incidents of human cancers. In cellular and animal experimental systems, arsenic has been shown to be highly capable of activating several signaling pathways that play critical roles in cell growth regulation, malignant transformation and the stemness of cancer stem-like cells. Emerging evidence indicates certain oncogenic properties of the Nrf2 transcription factor that can be activated by arsenic and many other environmental hazards. In human bronchial epithelial cells, our most recent data suggested that arsenic-activated Nrf2 signaling fosters metabolic reprogramming of the cells through shifting mitochondrial TCA cycle to cytosolic glycolysis, and some of the metabolites in glycolysis shunt the hexosamine biosynthesis and serine-glycine pathways important for the energy metabolism of the cancer cells. In the current report, we further demonstrated direct regulation of oncogenic signals by arsenic-activated Nrf2 and connection of Nrf2 with ATF3 stress transcription factor. Meanwhile, we also highlighted some unanswered questions on the molecular characteristics of the Nrf2 protein, which warrants further collaborative efforts among scientists for understanding the important role of Nrf2 in human cancers either associated or not to environmental arsenic exposure.

Flower Mimics Roll Out Multicolored Carpets to Lure and Kill the House Fly

Flowers and their spatial clustering are important parameters that mediate the foraging behavior and visitation rate of pollinating insects. Visual stimuli are crucial for triggering behavioral changes in the house fly, Musca domestica, which regularly visits plants for feeding and reproduction. The success of bait technology, which is the principal means of combatting flies, is adversely affected by reduced attractiveness and ineffective application techniques. Despite evidence that house flies have color vision capacity, respond to flowers, and exhibit color and pattern preference, the potential of artificial flowers as attractive factors has not been explored. The present study was performed to investigate whether artificial floral designs can lure and kill house flies. Starved wild house flies were presented with equal opportunities to acquire sugar meals, to which boric acid had been added as a toxin, from one flower arrangement (blue-dominated design, BDD; yellow-dominated design, YDD; or pink-dominated design, PDD), and a non-toxic white design (WDD). We also allowed house flies to forage within an enclosure containing two non-toxic floral designs (WDDs). The differences in mortality between the two environments with and without toxicant were examined. The survival rate of Musca domestica was extremely high when WDDs containing non-toxic sugar sources were the only feeding sites available. When given an option to forage in an environment containing a BDD and a WDD, house flies showed a high mortality rate (76%) compared to their counterparts maintained in the WDD environment (2%). When kept in an enclosure containing one YDD and a WDD, flies showed a mortality rate of 88%; however, no mortality occurred among flies confined to a compound with a WDD pair. When provided an even chance of foraging in an enclosure containing a mixed pair of floral arrangements (PDD and WDD) and another with two WDDs, flies showed a higher mortality rate (78%) in the first environment. However, the maximum survival rate (100%) was seen in the WDD environment. Exposure to YDD tended to result in a greater mortality rate than with the two other floral designs. Mortality gradually increased with time among flies exposed to tested artificial floral designs. The results presented here clearly indicated that artificial flower arrangements with a toxic sugar reward were strikingly attractive for house flies when their preferred color (white) was present. These observations offer novel possibilities for future development of flower mimic-based house fly control.

Recurring exposure to low humidity induces transcriptional and protein level changes in the vocal folds of rabbits

Voice disorders are an important human health condition. Hydration is a commonly recommended preventive measure for voice disorders though it is unclear how vocal fold dehydration is harmful at the cellular level. Airway surface dehydration can result from exposure to low humidity air. Here we have induced airway surface dehydration in New Zealand White rabbits exposed to a recurring 8-h low humidity environment over 15 days. This model mimics an occupational exposure to a low humidity environment. Exposure to moderate humidity was the control condition. Full thickness soft-tissue samples, including the vocal folds and surrounding laryngeal tissue, were collected for molecular analysis. RT-qPCR demonstrated a significant upregulation of MUC4 (mucin 4) and SCL26A9 (chloride channel) and a large fold-change though statistically non-significant upregulation of SCNNA1 (epithelial sodium channel). Proteomic analysis demonstrated differential regulation of proteins clustering into prospective functional groups of muscle structure and function, oxidative stress response, and protein chaperonin stress response. Together, the data demonstrate that recurring exposure to low humidity is sufficient to induce both transcriptional and translational level changes in laryngeal tissue and suggest that low humidity exposure induces cellular stress at the level of the vocal folds.

Interactions of Coated-Gold Engineered Nanoparticles with Aquatic Higher Plant Salvinia minima Baker

The study investigated the interactions of coated-gold engineered nanoparticles (nAu) with the aquatic higher plant Salvinia minima Baker in 2,7, and 14 d. Herein, the nAu concentration of 1000 µg/L was used; as in lower concentrations, analytical limitations persisted but >1000 µg/L were deemed too high and unlikely to be present in the environment. Exposure of S. minima to 1000 µg/L of citrate (cit)- and branched polyethyleneimine (BPEI)-coated nAu (5, 20, and 40 nm) in 10% Hoagland’s medium (10 HM) had marginal effect on biomass and growth rate irrespective of nAu size, coating type, or exposure duration. Further, results demonstrated that nAu were adsorbed on the plants’ roots irrespective of their size or coating variant; however, no evidence of internalization was apparent, and this was attributed to high agglomeration of nAu in 10 HM. Hence, adsorption was concluded as the basic mechanism of nAu accumulation by S. minima. Overall, the long-term exposure of S. minima to nAu did not inhibit plant biomass and growth rate but agglomerates on plant roots may block cell wall pores, and, in turn, alter uptake of essential macronutrients in plants, thus potentially affecting the overall ecological function.

Aquatic Environment Exposure and Toxicity of Engineered Nanomaterials Released from Nano-Enabled Products: Current Status and Data Needs

Rapid commercialisation of nano-enabled products (NEPs) elevates the potential environmental release of engineered nanomaterials (ENMs) along the product life cycle. The current review examined the state of the art literature on aquatic environment exposure and ecotoxicity of product released (PR) engineered nanomaterials (PR–ENMs). Additionally, the data obtained were applied to estimate the risk posed by PR–ENMs to various trophic levels of aquatic biota as a means of identifying priority NEPs cases that may require attention with regards to examining environmental implications. Overall, the PR–ENMs are predominantly associated with the matrix of the respective NEPs, a factor that often hinders proper isolation of nano-driven toxicity effects. Nevertheless, some studies have attributed the toxicity basis of observed adverse effects to a combination of the released ions, ENMs and other components of NEPs. Notwithstanding the limitation of current ecotoxicology data limitations, the risk estimated herein points to an elevated risk towards fish arising from fabrics’ PR–nAg, and the considerable potential effects from sunscreens’ PR–nZnO and PR–nTiO2 to algae, echinoderms, and crustaceans (PR–nZnO), whereas PR–nTiO2 poses no significant risk to echinoderms. Considering that the current data limitations will not be overcome immediately, we recommend the careful application of similar risk estimation to isolate/prioritise cases of NEPs for detailed characterisation of ENMs’ release and effects in aquatic environments.