165 THE EFFECT OF INDIGENOUS CHICKEN EGG YOLK SOURCES AND TEMPERATURES ON SHORT-TERM PRESERVATION OF SOUTH AFRICAN INDIGENOUS GOAT SEMEN

2017 ◽  
Vol 29 (1) ◽  
pp. 191
Author(s):  
M. A. Bopape ◽  
T. L. Nedambale ◽  
C. M. Pilane ◽  
K. C. Lehloenya
Keyword(s):  
South African ◽  
Sperm Motility ◽  
Egg Yolk ◽  
White Leghorn ◽  
Short Term ◽  
Indigenous Chicken ◽  
Treatment Groups ◽  
Chicken Egg ◽  
Indigenous Goat ◽  
Chicken Egg Yolk

Egg yolk is a common constituent of semen extender and protects sperm against cold shock. Besides its protective characteristic, chicken egg yolk is mostly included in extenders for semen preservation due to its abundant availability. The aim of the study was to compare egg yolk sources from different indigenous chicken egg yolk sources and storage temperatures on short-term preservation of South African indigenous goat semen. Semen was collected from 8 South African indigenous goats with artificial vagina during the breeding season (autumn). From each of the 8 goats, 6 replicates were done. Semen samples were randomly allocated into 4 treatment groups of Tris-based extenders containing 20% of egg yolk from White Leghorn as a control, Ovambo, Potchefstroom Koekoek, and Venda chicken breeds. The extended semen samples were stored at 5 or 25°C for 48 h. Semen samples were evaluated for sperm motility using computer-aided sperm analysis sperm viability and morphology with fluorescence microscope at 0, 3, 24, and 48 h. Data was analysed with ANOVA using Stata® version 12 (StataCorp, College Station, TX, USA) statistical software to test the differences between the treatments. The total, progressive, and rapid sperm motility rates were higher in freshly collected semen (90.6, 42.7, and 33.0%, respectively) compared with treatment groups. Semen extended with Tris without egg yolk had higher total sperm motility rate at both 5°C (48.1; 51.1%) and 25°C (43.3; 53.3%) temperatures for 48 h. Semen extended with egg yolk from different egg yolk sources had no sperm motility from 24 h when stored at 25°C. Semen extended with Tris without egg yolk (48.1%) had higher sperm motility than Ovambo, Potchefstroom Koekoek, Venda, and White Leghorn (3.7, 0, 0, and 0.4%, respectively) when stored at 5°C for 48 h. However, sperm motility declined when storage increased. In conclusion, addition of egg yolk had no effect on preserving goat semen. However, Tris-based extender without addition of egg yolk preserved semen for longer period than semen extended with egg yolk regardless of egg yolk origin or chicken breed.

38 QUAIL EGG YOLK IN CITRATE EXTENDER IS SUITABLE FOR CRYOPRESERVATION OF NGUNI BULL SEMEN

2016 ◽  
Vol 28 (2) ◽  
pp. 149
Author(s):  
M. M. Seshoka ◽  
M. L. Mphaphathi ◽  
K. S. Mafolo ◽  
M. Nkadimeng ◽  
Z. C. Raphalalani ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Egg Yolk ◽  
Sperm Analysis ◽  
Chicken Egg ◽  
Bull Semen ◽  
Significant Difference ◽  
Computer Aided ◽  
Chicken Egg Yolk ◽  
Fresh Semen ◽  
Motility Rate

Traditionally, commercial hen egg yolk has been used in extenders or freezing media because of its easy availability. However, the use of quail egg yolk has not been used for preserving Nguni bull semen. The aim of the study was to compare the suitability of different quail egg yolk concentrations (5, 10, 15, and 20%) for cryopreserving Nguni bull semen. Semen was collected from 14 stud Nguni bulls with the aid of electro ejaculator. Collected semen samples were kept in a thermos-flask containing warm water at a temperature of 37°C and transported to the laboratory for further analyses. The sperm motility traits were evaluated using computer-aided sperm analysis prior extension. Semen samples were then randomly allocated into 5, 10, 15, and 20% of quail egg yolk and 20% concentration of chicken egg yolk (control) in citrate extender. The extender was supplemented with 12% of glycerol (Seshoka et al. 2012) as a cryoprotectant, and semen samples were diluted (1 : 2) and equilibrated for a period of 4 h at 5°C. After equilibration, semen samples were loaded into 0.25-mL straws, placed into a controlled rate programmable freezer, and stored in a LN tank (–196°C) until thawing. Frozen semen straws were thawed in a water bath at 37°C for 1 min. Thawed semen was evaluated for sperm motility traits using a computer-aided sperm analysis system. Data were analysed with ANOVA. A significant difference was recorded between fresh total sperm motility rate (99%) and frozen-thawed semen samples with either 5% (87.3%) quail or 20% (87.6%) chicken egg yolk citrate extender compared with 10% (92.6%), 15% (91.2%), or 20% (89.9%) quail egg yolk citrate extender. Moreover, fresh semen also resulted in a significantly higher progressive sperm motility rate (39.3%) as compared with frozen-thawed with 5% (26.2%) or 20% quail (28.5%) or 20% chicken (22.7%) egg yolk citrate extender. The results also demonstrated that the use of 10, 15, and 20% quail egg yolk in citrate extender yielded comparable results on total sperm motility with fresh semen as compared with 5% quail and 20% chicken egg yolk. In conclusion, quail egg yolk extender provided sufficient cryo-effectiveness to cryopreservation of Nguni bull semen.

scholarly journals Motilitas dan vitalitas spermatozoa manusia post freezing setelah simpan beku dengan medium TES-Tris yolk citrat yang dimodifikasi dengan jenis kuning telur berbeda

2012 ◽  
Vol 14 (1) ◽  
pp. 37
Author(s):  
Muhammad Anwar Djaelani
Keyword(s):  
Initial Data ◽  
Key Words ◽  
Sperm Motility ◽  
Egg Yolk ◽  
The Other ◽  
Inclusion Criteria ◽  
Who Criteria ◽  
Chicken Egg ◽  
Duck Egg ◽  
Chicken Egg Yolk

The aim of this research was to examine the possibility of semen cryopreservation using modified TES-Tris yolk citrat (TES-TYC) medium with different egg yolk. Semen fulfilling inclusion  criteria with WHO  criteria was divided into three groups and the sperm motility and vitality was counted as initial data. The semen was then mixed with TES-TYC medium with chicken egg yolk, TES-TYC medium with duck egg yolk and TES-TYC medium with goose egg yolk then cryopreserved in liquid nitrogen. After one mounth the semen was thawed and recount its sperm motility and vitality. Data obtained showed that the motility and vitality of  post freezing sperm cryopreserved with TES-TYC medium with chiken was higher compared to the other medium. It could be concluded that the existenced of chicken egg yolk in TES-TYC medium was better kept sperm integrity during cryopreservation compared with the other medium, hence the existenced of egg yolk as ingredient in TES-TYC medium should be chicken egg yolk.   Key words : motilitas dan vitalitas, medium TES-Tris yolk citrat, kuning telur

scholarly journals Comparative study of chicken egg yolk and quail egg yolk in two chilled canine semen extenders

2020 ◽  
Vol 17 (4) ◽  
pp. 62-69
Author(s):  
S.A. Ubah ◽  
M. Sule ◽  
I.C. Chibuogwu ◽  
P.K. Columbus ◽  
K.O. Abah ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Egg Yolk ◽  
Chicken Egg ◽  
Significant Difference ◽  
Skimmed Milk ◽  
Mass Activity ◽  
The Difference ◽  
Chicken Egg Yolk

The aim of this work was to substitute chicken egg yolk with quail egg yolk in two semen extenders and to evaluate the quality of the extended canine semen following chilled storage. Semen was pooled from male dogs (n= 4) of about 18-months old and body weight of about 28 kg. Four extenders were tested: (1) tris buffered chicken egg yolk extender (2) tris buffered quail egg yolk extender, (3) skimmed milk chicken egg yolk extender and (4) skimmed milk quail egg yolk extender. Semen was diluted with corresponding extender in the ratio 1:4. The diluted semen samples were analyzed for motility, mass activity, viability, abnormalities percentage and pH for three consecutive days. There was no significant difference (P>0.05) between chicken egg yolk and quail egg yolk in either tris diluent or skimmed milk extender with respect to pH, mass activity and sperm motility. Samples stored in both the tris and skimmed milk-based extenders with quail egg yolk displayed greater viability than those in chicken egg yolk but the difference was not significant (P>0.05). Viability, mass activity and sperm motility decreased as treatment days increased in both chicken and quail egg yolk extenders. Results showed that a pH of 6.5 was maintained from day 0 to day 3. There was no difference in semen quality between chicken and quail egg yolk in either the tris diluent or skimmed milk extender (P> 0.05). It was recommended that quail egg yolk could be substituted for chicken egg yolk in the two canine semen extenders. Further modifications of the diluents with quail egg yolk might produce an improved result. Keywords: Canine, Chicken Chilled, Egg yolk, Extenders, Quail, Semen

Chicken egg yolk antibodies (IgY)-based antivenom for neutralization of snake venoms: a review

Toxin Reviews ◽  
2021 ◽  
pp. 1-12
Author(s):  
Ankit Choraria ◽  
Rajeswari Somasundaram ◽  
S. Janani ◽  
Selvakumar Rajendran ◽  
Naoual Oukkache ◽  
...  
Keyword(s):  
Egg Yolk ◽  
Snake Venoms ◽  
Chicken Egg ◽  
Egg Yolk Antibodies ◽  
Chicken Egg Yolk

Development of viper-venom antibodies in chicken egg yolk and assay of their antigen binding capacity

Toxicon ◽  
2002 ◽  
Vol 40 (7) ◽  
pp. 857-861 ◽  
Author(s):  
C Maya Devi ◽  
M Vasantha Bai ◽  
L.K Krishnan
Keyword(s):  
Binding Capacity ◽  
Egg Yolk ◽  
Antigen Binding ◽  
Chicken Egg ◽  
Chicken Egg Yolk

Isolation of Immunoglobulin from Chicken Egg Yolk using Single-Stage Ultrafiltration with 100-kDa Regenerated Cellulose Membranes

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Jianguo Liu ◽  
Juan Yang ◽  
Hai Xu ◽  
Hu Zhu ◽  
Jianbo Qu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Egg Yolk ◽  
Single Stage ◽  
Regenerated Cellulose ◽  
Cellulose Membrane ◽  
Permeate Flux ◽  
Operational Parameters ◽  
Solution Ph ◽  
Chicken Egg ◽  
Chicken Egg Yolk

The aim of this work is to develop a membrane-based cost-effective process for the rapid isolation of immunoglobulin from chicken egg yolk. It was found that a single-stage ultrafiltration using a 100 kDa molecular weight cut-off regenerated cellulose membrane could be employed to isolate immunoglobulin from the crude feedstock. The effects of operational parameters (solution pH, ionic strength, stirring speed and permeate flux) on the transmission of immunoglobulin and the presence of impurity protein with molecular weight close to immunoglobulin were quantified using the parameter scanning ultrafiltration technique. Under optimized conditions, the purity of immunoglobulin obtained was about 85 percent after the single-stage ultrafiltration process, and the recovery of immunoglobulin from the feedstock was 91 percent.

scholarly journals A simple method for isolating chicken egg yolk immunoglobulin using effective delipidation solution and ammonium sulfate

2015 ◽  
Vol 94 (1) ◽  
pp. 104-110 ◽  
Author(s):  
Chenyao Tong ◽  
Fang Geng ◽  
Zhenjiao He ◽  
Zhaoxia Cai ◽  
Meihu Ma
Keyword(s):  
Ammonium Sulfate ◽  
Egg Yolk ◽  
Simple Method ◽  
Chicken Egg ◽  
Egg Yolk Immunoglobulin ◽  
Chicken Egg Yolk

scholarly journals COMPARISON OF ANTIVENOM POTENTIAL OF CHICKEN EGG YOLK ANTIBODIES GENERATED AGAINST BENTONITE AND ADJUVENT COATED VENOMES OF COMMON POISONOUS SNAKE IN INDIA

1970 ◽  
Vol 7 (1) ◽  
pp. 259-267 ◽  
Author(s):  
S. Meenatchisundaram ◽  
R. Selvakumaran ◽  
G. Parameswari ◽  
A. Michael
Keyword(s):  
Room Temperature ◽  
Deae Cellulose ◽  
Egg Yolk ◽  
Precipitation Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Snake Venoms ◽  
Chicken Egg ◽  
Ammonium Sulphate Precipitation ◽  
Egg Yolk Antibodies ◽  
Chicken Egg Yolk

Antivenom antibodies were generated in white leghorn chicken against bentonite and adjuvant coated venoms of Common Indian Poisonous Snakes (Cobra, Krait, Russell's viper and Saw Scaled viper).The antivenom from immunized chicken egg yolk were purified by polyethylene glycol (PEG) and ammonium sulphate precipitation method and further purified by DEAE cellulose ion exchange column chromatography and concentrated by polyvinyl pyrolidone powder at room temperature. The titer of antibodies was estimated using Enzyme Linked Immunosorbent Assay (ELISA).The chickens immunized with Freund's complete adjuvant showed slightly higher titre when compared to bentonite. Inhibition of lethal, edema, haemorrhagic, procoagulant and phospholipase A2 and fibrinolytic activities of snake venoms were determined. The chicken egg yolk antivenom was effective in neutralization of these toxic and enzymatic activities of venom. The median effective dose (ED50) of chicken egg yolk antibodies raised against adjuvant coated venoms showed effective neutralizing venom toxicity when compared to the antibodies raised using bentonite coated venoms.

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