scholarly journals Brain function and macromolecules, IV. Uridine incorporation into polysomes of mouse brain during different behavioral experiences.

1968 ◽  
Vol 61 (3) ◽  
pp. 917-922 ◽  
Author(s):  
L. B. Adair ◽  
J. E. Wilson ◽  
E. Glassman
Keyword(s):  
Mouse Brain ◽  
Brain Function ◽  
Uridine Incorporation

scholarly journals Imaging Sterols and Oxysterols in Mouse Brain Reveals Distinct Spatial Cholesterol Metabolism

2018 ◽  
Author(s):  
Eylan Yutuc ◽  
Roberto Angelini ◽  
Mark Baumert ◽  
Natalia Mast ◽  
Irina Pikuleva ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Brain Function ◽  
Mass Spectrometry Imaging ◽  
Cholesterol Metabolism ◽  
Biologically Active ◽  
Wild Type ◽  
Tissue Slices ◽  
The Brain

AbstractDysregulated cholesterol metabolism is implicated in a number of neurological disorders. Many sterols, including cholesterol and its precursors and metabolites, are biologically active and important for proper brain function. However, spatial cholesterol metabolism in brain and the resulting sterol distributions are poorly defined. To better understand cholesterol metabolism in situ across the complex functional regions of brain, we have developed on-tissue enzyme-assisted derivatisation in combination with micro-liquid-extraction for surface analysis and liquid chromatography - mass spectrometry to image sterols in tissue slices (10 µm) of mouse brain. The method provides sterolomic analysis at 400 µm spot diameter with a limit of quantification of 0.01 ng/mm2. It overcomes the limitations of previous mass spectrometry imaging techniques in analysis of low abundance and difficult to ionise sterol molecules, allowing isomer differentiation and structure identification. Here we demonstrate the spatial distribution and quantification of multiple sterols involved in cholesterol metabolic pathways in wild type and cholesterol 24S-hydroxylase knock-out mouse brain. The technology described provides a powerful tool for future studies of spatial cholesterol metabolism in healthy and diseased tissues.SignificanceThe brain is a remarkably complex organ and cholesterol homeostasis underpins brain function. It is known that cholesterol is not evenly distributed across different brain regions, however, the precise map of cholesterol metabolism in the brain remains unclear. If cholesterol metabolism is to be correlated with brain function it is essential to generate such a map. Here we describe an advanced mass spectrometry imaging platform to reveal spatial cholesterol metabolism in situ at 400 µm resolution on 10 µm tissue slices from mouse brain. We mapped, not only cholesterol, but also other biologically active sterols arising from cholesterol turnover in both wild type and mice lacking cholesterol 24-hydroxylase (Cyp46a1), the major cholesterol metabolising enzyme.

Three-dimensional inversion recovery manganese-enhanced MRI of mouse brain using super-resolution reconstruction to visualize nuclei involved in higher brain function

2014 ◽  
Vol 27 (7) ◽  
pp. 749-759 ◽  
Author(s):  
Dana S. Poole ◽  
Esben Plenge ◽  
Dirk H. J. Poot ◽  
Egbert A. J. F. Lakke ◽  
Wiro J. Niessen ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Brain Function ◽  
Three Dimensional ◽  
Super Resolution ◽  
Inversion Recovery ◽  
Enhanced Mri ◽  
Manganese Enhanced Mri

L23 Effects of peripheral (co-)administration of liraglutide and ghrelin in the R6/2 mouse brain function

2016 ◽  
Vol 87 (Suppl 1) ◽  
pp. A98.1-A98
Author(s):  
Ana I Duarte ◽  
Marie Sjögren ◽  
Maria S Santos ◽  
Catarina R Oliveira ◽  
Paula I Moreira ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Brain Function

Monitoring of Blood Flow by Drug Stimulation on Mouse Brain Using Non-Invasive Photoacoustic Imaging Technology

2007 ◽  
Vol 364-366 ◽  
pp. 1123-1127
Author(s):  
Shi Hua Yang ◽  
Ye Qi Lao
Keyword(s):  
Blood Flow ◽  
Mouse Brain ◽  
Light Absorption ◽  
Brain Function ◽  
Photoacoustic Imaging ◽  
Non Invasive ◽  
Mouse Cerebral Cortex ◽  
Flow Changes ◽  
The Brain

The highlight of photoacosutic imaging (PAI) is a method that combines ultrasonic resolution with high contrast due to light absorption. Photoacoustic signals carry the information of the light absorption distribution of biological tissue, which is often related to its character of structure, physiological and pathological changes because of different physiology conditions in response to different light absorption coefficients. A non-invasive PAI system was developed and successfully acquired in vivo images of mouse brain. Based on the intrinsic PA signals from the brain, the vascular network and the detailed structures of the mouse cerebral cortex were clearly visualized. The ability of PAI monitoring of cerebral hemodynamics was also demonstrated by mapping of the mouse superficial cortex with and without drug stimulation. The extracted PA signals intensity profiles obviously testified that the cerebral blood flow (CBF) in the mouse brain was changed under the stimulation of acetazolamide (ACZ). The experimental results suggest that PAI can provide non-invasive images of blood flow changes, and has the potential for brain function detection.

scholarly journals Precision mapping of the mouse brain metabolome

2020 ◽  
Author(s):  
Huanhuan Pang ◽  
Jun-Liszt Li ◽  
Xiao-Ling Hu ◽  
Fei Chen ◽  
Xiaofei Gao ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Biological System ◽  
Brain Function ◽  
Brain Metabolism ◽  
Brain Regions ◽  
Targeted Metabolomics ◽  
Collection System ◽  
Comprehensive Knowledge ◽  
Health And Disease ◽  
Metabolomics Analysis

AbstractMetabolism is physiologically fundamental to a biological system. Understanding brain metabolism is critical for our comprehensive knowledge of brain function in health and disease. Combining a microarray collection system with targeted metabolomics analysis, here we performed precision mapping of the metabolome in the mouse brain and created maps for 79 metabolites with a resolution of 0.125mm3 per pixel (i.e., brain subregion). The metabolome atlas provides researchers with a useful resource to interpret the vulnerability of specific brain regions to various disease-relevant metabolic perturbations.

scholarly journals Brain function and macromolecules. I. Incorporation of uridine into RNA of mouse brain during short-term training experience.

1966 ◽  
Vol 55 (6) ◽  
pp. 1423-1431 ◽  
Author(s):  
J. W. Zemp ◽  
J. E. Wilson ◽  
K. Schlesinger ◽  
W. O. Boggan ◽  
E. Glassman
Keyword(s):  
Mouse Brain ◽  
Brain Function ◽  
Short Term ◽  
Training Experience

scholarly journals Neuronal morphology and projection analysis by multispectral tracing in densely labeled mouse brain

2017 ◽  
Author(s):  
Douglas H. Roossien ◽  
John M. Webb ◽  
Benjamin V. Sadis ◽  
Yan Yan ◽  
Lia Y. Min ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Long Range ◽  
Mouse Brain ◽  
Cell Morphology ◽  
Brain Function ◽  
Neuronal Morphology ◽  
Neuronal Populations ◽  
Sparse Labeling ◽  
Projection Analysis ◽  
Imagej Plugin

AbstractAccurate and complete neuronal wiring diagrams are necessary for understanding brain function at many scales from long-range interregional projections to microcircuits. Traditionally, light microscopy-based anatomical reconstructions use monochromatic labeling and therefore necessitate sparse labeling to eliminate tracing ambiguity between intermingled neurons. Consequently, our knowledge of neuronal morphology has largely been based on averaged estimations across many samples. Recently developed second-generation Brainbow tools promise to circumvent this limitation by revealing fine anatomical details of many unambiguously identifiable neurons in densely labeled samples. Yet, a means to quantify and analyze the information is currently lacking. Therefore, we developed nTracer, an ImageJ plugin capable of rapidly and accurately reconstructing whole-cell morphology of large neuronal populations in densely labeled brains.

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