scholarly journals Induction of Mitochondrial DNA Synthesis in Monkey Cells Infected by Simian Virus 40 and (or) Treated with Calf Serum

1971 ◽  
Vol 68 (4) ◽  
pp. 717-720 ◽  
Author(s):  
A. J. Levine
Keyword(s):  
Mitochondrial Dna ◽  
Dna Synthesis ◽  
Calf Serum ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Mitochondrial Dna Synthesis

scholarly journals Phosphate and the regulation of DNA replication in normal and virus-transformed 3T3 cells

1983 ◽  
Vol 214 (3) ◽  
pp. 695-702 ◽  
Author(s):  
W Engström ◽  
A Zetterberg
Keyword(s):  
Dna Synthesis ◽  
Time Course ◽  
Simian Virus 40 ◽  
Complete Removal ◽  
Simian Virus ◽  
Phosphate Concentration ◽  
Serum Starvation ◽  
Cell Multiplication ◽  
Inhibitory Effects ◽  
3T3 Cells

3T3 cells were cultured in media with different phosphate concentrations and the effects on DNA synthesis were examined. Even a modest phosphate depletion markedly inhibited DNA synthesis and cell multiplication in proliferating cultures. Furthermore, the decrease in the proportion of DNA-synthesizing cells observed after phosphate starvation followed the same time-course as the decrease seen after serum starvation. Cells starved to quiescence in a medium with a 100-fold decrease in phosphate concentration remained viable but non-proliferating for up to 3 weeks, i.e. they had entered a state of quiescence comparable with that seen after serum starvation. Addition of phosphate to phosphate-depleted cultures restored DNA synthesis within 24h. Furthermore, the kinetics of [3H]thymidine labelling after phosphate addition were nearly identical with the labelling kinetics following addition of serum to serum-depleted cultures. In contrast, phosphate deprivation had no inhibitory effects on DNA synthesis in simian-virus-40-transformed 3T3 cells. Furthermore, the inhibitory effects on DNA synthesis in such cells caused by a complete removal of serum could not be further enhanced by decreasing the phosphate concentration in the culture medium.

Replication of cloned DNA containing the Alu family sequence during cell extract-promoting simian virus 40 DNA synthesis

1984 ◽  
Vol 4 (8) ◽  
pp. 1476-1482
Author(s):  
H Ariga
Keyword(s):  
Dna Replication ◽  
Dna Synthesis ◽  
Rna Polymerase Iii ◽  
Simian Virus 40 ◽  
Cell Extract ◽  
Simian Virus ◽  
T Antigen ◽  
Sv40 T Antigen ◽  
Sv40 Dna

The replicating activity of several cloned DNAs containing putative origin sequences was examined in a cell-free extract that absolutely depends on simian virus 40 (SV40) T antigen promoting initiation of SV40 DNA replication in vitro. Of the three DNAs containing the human Alu family sequence (BLUR8), the origin of (Saccharomyces cerevisiae plasmid 2 micron DNA (pJD29), and the yeast autonomous replicating sequence (YRp7), only BLUR8 was active as a template. Replication in a reaction mixture with BLUR8 as a template was semiconservative and not primed by a putative RNA polymerase III transcript synthesized on the Alu family sequence in vitro. Pulse-chase experiments showed that the small-sized DNA produced in a short-term incubation was converted to full-length closed circular and open circular DNAs in alkaline sucrose gradients. DNA synthesis in extracts began in a region of the Alu family sequence and was inhibited 80% by the addition of anti-T serum. Furthermore, partially purified T antigen bound the Alu family sequence in BLUR8 by the DNA-binding immunoassay. These results suggest that SV40 T antigen recognizes the Alu family sequence, similar to the origin sequence of SV40 DNA, and initiates semiconservative DNA replication in vitro.

scholarly journals Binding of a sequence-specific single-stranded DNA-binding factor to the simian virus 40 core origin inverted repeat domain is cell cycle regulated.

1993 ◽  
Vol 13 (1) ◽  
pp. 408-420 ◽  
Author(s):  
E P Carmichael ◽  
J M Roome ◽  
A F Wahl
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Dna Binding ◽  
Dna Synthesis ◽  
Inverted Repeat ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Single Stranded Dna ◽  
Repeat Domain

The inverted repeat domain (IR domain) within the simian virus 40 origin of replication is the site of initial DNA melting prior to the onset of DNA synthesis. The domain had previously been shown to be bound by a cellular factor in response to DNA damage. We demonstrate that two distinct cellular components bind opposite strands of the IR domain. Replication protein A (RPA), previously identified as a single-stranded DNA binding protein required for origin-specific DNA replication in vitro, is shown to have a preference for the pyrimidine-rich strand. A newly described component, IR factor B (IRF-B), specifically recognizes the opposite strand. IRF-B binding activity in nuclear extract varies significantly with cell proliferation and the cell cycle, so that binding of IRF-B to the IR domain is negatively correlated with the onset of DNA synthesis. Loss of IRF-B binding from the nucleus also occurs in response to cellular DNA damage. UV cross-linking indicates that the core binding component of IRF-B is a protein of ca. 34 kDa. We propose that RPA and IRF-B bind opposite strands of the IR domain and together may function in the regulation of origin activation.

scholarly journals Influence of hydroxyurea on the course of germination and growth of rape (Brasica napus L.) seedlings

2015 ◽  
Vol 47 (1–2) ◽  
pp. 131-141 ◽  
Author(s):  
M. Kuraś ◽  
H. Teleżyński
Keyword(s):  
Mitochondrial Dna ◽  
Root Growth ◽  
Dna Synthesis ◽  
Elongation Growth ◽  
Complete Inhibition ◽  
Hypocotyl Growth ◽  
Prolonged Incubation ◽  
Inhibition Of Growth ◽  
Germination And Growth ◽  
Mitochondrial Dna Synthesis

The effects of continuous incubation in hydroxyurea (HU) solutions (0.2, 0.4, 0.8 mg/ml) on germination of rape seeds and growth of young seedling axes were studied during 132 hours from initial soaking. Germination turned out to be unaffected by the treatment. Root growth was first increasingly inhibited by the HU concentration tested, but after prolonged incubation a complete arrest of the root growth was noted at all HU concentrations. The elongation growth of hypocotyls was found to be stimulated by a HU 0.2 mg/ml concentration while it was markedly suppressed by 0.4 mg/ml, and completely arrested by 0.8 mg/ml Inhibition of growth of the upright hypocotyl part at higher HU concentration was found to be accompanied by the unbending of the hooked under-cotyledonary part. It is suggested that inhibition of nuclear endomitotic DNA synthesis In elongating hypocotyl cells, suppresses only partially their growth, whereas a complete inhibition of the hypocotyl growth results from arrest of the mitochondrial DNA synthesis.

scholarly journals Hormonal regulation of the transformation phenotype in simian virus 40-transformed rat embryonic preadipose cell lines.

1984 ◽  
Vol 4 (4) ◽  
pp. 712-721 ◽  
Author(s):  
S Yasumoto
Keyword(s):  
Cell Density ◽  
Cell Lines ◽  
Hormonal Regulation ◽  
Growth Regulation ◽  
High Cell Density ◽  
Calf Serum ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
High Cell

The regulation of transformed phenotypes was studied in newly isolated preadipose cell lines which were established after infection with simian virus 40 tsA58 dl2009. The clonal cell lines isolated exhibited most of the characteristics typical of transformed cells. The transformants, however, were able to differentiate into adipocytes in the presence of low calf serum (0.5%) and a combination of several hormones, including hydrocortisone and insulin. Treatment with insulin alone stimulated the growth of these cells but did not induce lipid accumulation without added hydrocortisone. The effect of hydrocortisone was accompanied by a restoration of growth control in the transformants after they reached high cell density. The blot hybridization analysis of cellular DNAs digested by restriction enzymes revealed that simian virus 40 genomes were integrated at multiple separate sites at which a head-to-tail oligomeric insertion took place. Large T antigen was synthesized in growing cells but was regulated at high cell density when cells were committed to differentiate by glucocorticoids. These results suggest that the glucocorticoid hydrocortisone is capable of restoring growth regulation at high cell densities to simian virus 40-transformed preadipose cell lines.

Functional simian virus 40 T antigen is expressed in hybrid cells having finite proliferative potential

1987 ◽  
Vol 7 (4) ◽  
pp. 1541-1544
Author(s):  
O M Pereira-Smith ◽  
J R Smith
Keyword(s):  
Dna Synthesis ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Transformed Cells ◽  
Proliferative Potential ◽  
Hybrid Cells ◽  
Ras Oncogenes ◽  
Immortal Cells ◽  
Cellular Dna

Simian virus 40-transformed human cells fused with other independently derived simian virus 40-transformed cells and tumor-derived cells containing activated H-ras and N-ras oncogenes yielded hybrids capable of indefinite division. Fusions with various other immortal cells yielded hybrids that had limited division potential. T antigen expressed in limited-division hybrids was functional for the induction of cellular DNA synthesis.

scholarly journals New mitochondrial DNA synthesis enables NLRP3 inflammasome activation

Nature ◽  
2018 ◽  
Vol 560 (7717) ◽  
pp. 198-203 ◽  
Author(s):  
Zhenyu Zhong ◽  
Shuang Liang ◽  
Elsa Sanchez-Lopez ◽  
Feng He ◽  
Shabnam Shalapour ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Dna Synthesis ◽  
Nlrp3 Inflammasome ◽  
Inflammasome Activation ◽  
Nlrp3 Inflammasome Activation ◽  
Mitochondrial Dna Synthesis
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