scholarly journals Substance P receptor binding sites are expressed by glia in vivo after neuronal injury.

1989 ◽  
Vol 86 (13) ◽  
pp. 5193-5197 ◽  
Author(s):  
P. W. Mantyh ◽  
D. J. Johnson ◽  
C. G. Boehmer ◽  
M. D. Catton ◽  
H. V. Vinters ◽  
...  
Keyword(s):  
Substance P ◽  
Receptor Binding ◽  
Binding Sites ◽  
Neuronal Injury ◽  
Substance P Receptor

scholarly journals Androgen and glucocorticoid receptor direct distinct transcriptional programs by receptor-specific and shared DNA binding sites

2021 ◽  
Vol 49 (7) ◽  
pp. 3856-3875
Author(s):  
Marina Kulik ◽  
Melissa Bothe ◽  
Gözde Kibar ◽  
Alisa Fuchs ◽  
Stefanie Schöne ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Transcription Factor ◽  
Dna Binding ◽  
Receptor Binding ◽  
Binding Sites ◽  
Specific Gene ◽  
Functional Diversification ◽  
Enhancer Activity ◽  
Sequence Composition

Abstract The glucocorticoid (GR) and androgen (AR) receptors execute unique functions in vivo, yet have nearly identical DNA binding specificities. To identify mechanisms that facilitate functional diversification among these transcription factor paralogs, we studied them in an equivalent cellular context. Analysis of chromatin and sequence suggest that divergent binding, and corresponding gene regulation, are driven by different abilities of AR and GR to interact with relatively inaccessible chromatin. Divergent genomic binding patterns can also be the result of subtle differences in DNA binding preference between AR and GR. Furthermore, the sequence composition of large regions (>10 kb) surrounding selectively occupied binding sites differs significantly, indicating a role for the sequence environment in guiding AR and GR to distinct binding sites. The comparison of binding sites that are shared shows that the specificity paradox can also be resolved by differences in the events that occur downstream of receptor binding. Specifically, shared binding sites display receptor-specific enhancer activity, cofactor recruitment and changes in histone modifications. Genomic deletion of shared binding sites demonstrates their contribution to directing receptor-specific gene regulation. Together, these data suggest that differences in genomic occupancy as well as divergence in the events that occur downstream of receptor binding direct functional diversification among transcription factor paralogs.

scholarly journals Two Populations of Glucocorticoid Receptor-Binding Sites in the Male Rat Hippocampal Genome

Endocrinology ◽  
2013 ◽  
Vol 154 (5) ◽  
pp. 1832-1844 ◽  
Author(s):  
J. Annelies E. Polman ◽  
E. Ronald de Kloet ◽  
Nicole A. Datson
Keyword(s):  
Receptor Binding ◽  
Binding Sites ◽  
Glucocorticoid Receptors ◽  
Full Range ◽  
Male Rat ◽  
Motif Analysis ◽  
Wide Range ◽  
Neuronal Processes ◽  
Almost All

Abstract In the present study, genomic binding sites of glucocorticoid receptors (GR) were identified in vivo in the rat hippocampus applying chromatin immunoprecipitation followed by next-generation sequencing. We identified 2470 significant GR-binding sites (GBS) and were able to confirm GR binding to a random selection of these GBS covering a wide range of P values. Analysis of the genomic distribution of the significant GBS revealed a high prevalence of intragenic GBS. Gene ontology clusters involved in neuronal plasticity and other essential neuronal processes were overrepresented among the genes harboring a GBS or located in the vicinity of a GBS. Male adrenalectomized rats were challenged with increasing doses of the GR agonist corticosterone (CORT) ranging from 3 to 3000 μg/kg, resulting in clear differences in the GR-binding profile to individual GBS. Two groups of GBS could be distinguished: a low-CORT group that displayed GR binding across the full range of CORT concentrations, and a second high-CORT group that displayed significant GR binding only after administering the highest concentration of CORT. All validated GBS, in both the low-CORT and high-CORT groups, displayed mineralocorticoid receptor binding, which remained relatively constant from 30 μg/kg CORT upward. Motif analysis revealed that almost all GBS contained a glucocorticoid response element resembling the consensus motif in literature. In addition, motifs corresponding with new potential GR-interacting proteins were identified, such as zinc finger and BTB domain containing 3 (Zbtb3) and CUP (CG11181 gene product from transcript CG11181-RB), which may be involved in GR-dependent transactivation and transrepression, respectively. In conclusion, our results highlight the existence of 2 populations of GBS in the rat hippocampal genome.

Substance P and substance K receptor binding sites in the human gastrointestinal tract: Localization by autoradiography

Peptides ◽  
1988 ◽  
Vol 9 (6) ◽  
pp. 1207-1219 ◽  
Author(s):  
T.S. Gates ◽  
R.P. Zimmerman ◽  
C.R. Mantyh ◽  
S.R. Vigna ◽  
J.E. Maggio ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Substance P ◽  
Receptor Binding ◽  
Binding Sites ◽  
Human Gastrointestinal Tract ◽  
Substance K

A comparison of in vivo and in vitro neuroimaging of 5-HT1A receptor binding sites in the cat brain

2006 ◽  
Vol 31 (3) ◽  
pp. 226-232 ◽  
Author(s):  
Nicolas Aznavour ◽  
Latifa Rbah ◽  
Lucienne Léger ◽  
Colette Buda ◽  
Jean-Pierre Sastre ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Binding Sites ◽  
Cat Brain

Comment on “Decreased Neurokinin-1 (Substance P) Receptor Binding in Patients with Panic Disorder: Positron Emission Tomographic Study With [18F]SPA-RQ”

2010 ◽  
Vol 67 (4) ◽  
pp. e25-e26
Author(s):  
Åsa Michelgård Palmquist ◽  
Anna Pissiota ◽  
Örjan Frans ◽  
Fredrik Åhs ◽  
Bengt Långström ◽  
...  
Keyword(s):  
Substance P ◽  
Panic Disorder ◽  
Receptor Binding ◽  
Positron Emission Tomographic ◽  
Positron Emission ◽  
Substance P Receptor ◽  
Neurokinin 1

Regulation of substance P receptor binding by GTP-binding proteins

1988 ◽  
Vol 22 (1-2) ◽  
pp. 37
Author(s):  
NormanD. Boyd ◽  
SusanG. Macdonald ◽  
Judith Luber-Narod ◽  
SusanE. Leeman
Keyword(s):  
Substance P ◽  
Receptor Binding ◽  
Binding Proteins ◽  
Gtp Binding Proteins ◽  
Gtp Binding ◽  
Substance P Receptor

scholarly journals Research Resource: Genome-Wide Mapping of in Vivo Androgen Receptor Binding Sites in Mouse Epididymis

2010 ◽  
Vol 24 (12) ◽  
pp. 2392-2405 ◽  
Author(s):  
Shuanggang Hu ◽  
Guangxin Yao ◽  
Xiaojun Guan ◽  
Zimei Ni ◽  
Wubin Ma ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Receptor Binding ◽  
Binding Sites ◽  
Genome Wide ◽  
Mouse Epididymis
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