scholarly journals Isolation of DNA fragments associated with methylated CpG islands in human adenocarcinomas of the lung using a methylated DNA binding column and denaturing gradient gel electrophoresis

1999 ◽  
Vol 96 (6) ◽  
pp. 2913-2918 ◽  
Author(s):  
M. Shiraishi ◽  
Y. H. Chuu ◽  
T. Sekiya
Keyword(s):  
Dna Binding ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Cpg Islands ◽  
Dna Fragments ◽  
Methylated Dna ◽  
Gradient Gel Electrophoresis

Segregation of partly melted molecules: isolation of CpG islands by polyacrylamide gel electrophoresis

2004 ◽  
Vol 385 (10) ◽  
pp. 967-973 ◽  
Author(s):  
Masahiko Shiraishi ◽  
Adam J. Oates ◽  
Xu Li ◽  
Ying H. Chuu ◽  
Takao Sekiya
Keyword(s):  
Efficient Method ◽  
Gel Electrophoresis ◽  
Human Cancer ◽  
Polyacrylamide Gel Electrophoresis ◽  
Cpg Islands ◽  
Theoretical Background ◽  
Dna Fragments ◽  
Practical Application ◽  
Gradient Gel Electrophoresis ◽  
Denaturant Gradient Gel Electrophoresis

Abstract The technique of segregation of partly melted molecules (SPM) is a convenient and efficient method to isolate DNA fragments associated with CpG islands. The approach is conceptually simple and uses denaturant gradient gel electrophoresis to separate DNA molecules digested with restriction endonucleases. The SPM methodology has successfully been applied to the identification of genes from anonymous, unsequenced DNA fragments and CpG islands methylated in human cancer. In this article the theoretical background and practical application of the SPM method is reviewed.

Allelopathic and Medicinal plant. 26. Millettia speciosa

2020 ◽  
Vol 51 (2) ◽  
pp. 125-146
Author(s):  
Nasiruddin Nasiruddin ◽  
Yu Zhangxin ◽  
Ting Zhao Chen Guangying ◽  
Minghui Ji
Keyword(s):  
Community Structure ◽  
Medicinal Plant ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Wiener Index ◽  
Pseudomonas Spp ◽  
Evenness Index ◽  
Gradient Gel Electrophoresis ◽  
Rhizosphere Pseudomonas

We grew cucumber in pots in greenhouse for 9-successive cropping cycles and analyzed the rhizosphere Pseudomonas spp. community structure and abundance by PCR-denaturing gradient gel electrophoresis and quantitative PCR. Results showed that continuous monocropping changed the cucumber rhizosphere Pseudomonas spp. community. The number of DGGE bands, Shannon-Wiener index and Evenness index decreased during the 3rd cropping and thereafter, increased up to the 7th cropping, however, however, afterwards they decreased again. The abundance of Pseudomonas spp. increased up to the 5th successive cropping and then decreased gradually. These findings indicated that the structure and abundance of Pseudomonas spp. community changed with long-term cucumber monocropping, which might be linked to soil sickness caused by its continuous monocropping.

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