scholarly journals Compensatory Energetic Relationships between Upstream Activators and the RNA Polymerase II General Transcription Machinery

1998 ◽  
Vol 273 (2) ◽  
pp. 932-939 ◽  
Author(s):  
Alison M. Lehman ◽  
Katharine B. Ellwood ◽  
Blake E. Middleton ◽  
Michael Carey
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Machinery ◽  
General Transcription Machinery

scholarly journals PTEN interacts with the transcription machinery on chromatin and regulates RNA polymerase II-mediated transcription

2019 ◽  
Vol 47 (11) ◽  
pp. 5573-5586 ◽  
Author(s):  
Nicole Steinbach ◽  
Dan Hasson ◽  
Deepti Mathur ◽  
Elias E Stratikopoulos ◽  
Ravi Sachidanandam ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Machinery

scholarly journals Recruitment of TREX to the Transcription Machinery by Its Direct Binding to the Phospho-CTD of RNA Polymerase II

PLoS Genetics ◽  
2013 ◽  
Vol 9 (11) ◽  
pp. e1003914 ◽  
Author(s):  
Dominik M. Meinel ◽  
Cornelia Burkert-Kautzsch ◽  
Anja Kieser ◽  
Eoghan O'Duibhir ◽  
Matthias Siebert ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Machinery ◽  
Direct Binding

scholarly journals Therapeutic Targeting of the General RNA Polymerase II Transcription Machinery

2020 ◽  
Vol 21 (9) ◽  
pp. 3354 ◽  
Author(s):  
Ryan D. Martin ◽  
Terence E. Hébert ◽  
Jason C. Tanny
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Complex Diseases ◽  
Cellular Models ◽  
Transcription Machinery ◽  
Transcription Inhibitors ◽  
Preclinical And Clinical Studies ◽  
Rna Polymerase Ii Transcription ◽  
Rnapii Transcription

Inhibitors targeting the general RNA polymerase II (RNAPII) transcription machinery are candidate therapeutics in cancer and other complex diseases. Here, we review the molecular targets and mechanisms of action of these compounds, framing them within the steps of RNAPII transcription. We discuss the effects of transcription inhibitors in vitro and in cellular models (with an emphasis on cancer), as well as their efficacy in preclinical and clinical studies. We also discuss the rationale for inhibiting broadly acting transcriptional regulators or RNAPII itself in complex diseases.

scholarly journals Quantitation of the RNA Polymerase II Transcription Machinery in Yeast

2001 ◽  
Vol 276 (50) ◽  
pp. 47150-47153 ◽  
Author(s):  
Tilman Borggrefe ◽  
Ralph Davis ◽  
Avital Bareket-Samish ◽  
Roger D. Kornberg
Keyword(s):  
Quantitative Analysis ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Blot Analysis ◽  
Dot Blot ◽  
Yeast Proteome ◽  
Transcription Machinery ◽  
Large Complex ◽  
Dot Blot Analysis ◽  
Rna Polymerase Ii Transcription

TAP tags and dot blot analysis have been used to measure the amounts of RNA polymerase II transcription proteins in crude yeast extracts. The measurements showed comparable amounts of RNA polymerase II, TFIIE, and TFIIF, lower levels of TBP and TFIIB, and still lower levels of Mediator and TFIIH. These findings are consistent with the presumed roles of the transcription proteins, but do not support the idea of their recruitment in a single large complex to RNA polymerase II promoters. The approach employed here can be readily extended to quantitative analysis of the entire yeast proteome.

The Eukaryotic Gene Transcription Machinery

2001 ◽  
Vol 382 (8) ◽  
pp. 1103-1107 ◽  
Author(s):  
Roger D. Kornberg
Keyword(s):  
Transcription Factors ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Gene Transcription ◽  
Atomic Resolution ◽  
Entire System ◽  
Transcription Machinery ◽  
General Transcription Factors ◽  
Eukaryotic Gene ◽  
Rna Polymerase Ii Transcription

Abstract Seven purified proteins may be combined to reconstitute regulated, promoterdependent RNA polymerase II transcription: five general transcription factors, Mediator, and RNA polymerase II. The entire system has been conserved across species from yeast to humans. The structure of RNA polymerase II, consisting of 10 polypeptides with a mass of about 500 kDa, has been determined at atomic resolution. On the basis of this structure, that of an actively transcribing RNA polymerase II complex has been determined as well.

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