scholarly journals Combining Precursor and Fragment Information for Improved Detection of Differential Abundance in Data Independent Acquisition

2019 ◽  
Vol 19 (2) ◽  
pp. 421-430 ◽  
Author(s):  
Ting Huang ◽  
Roland Bruderer ◽  
Jan Muntel ◽  
Yue Xuan ◽  
Olga Vitek ◽  
...  
Keyword(s):  
False Negative ◽  
Quantitative Information ◽  
Label Free ◽  
Combined Method ◽  
Data Independent Acquisition ◽  
Combined Use ◽  
Technological Developments ◽  
Individual Use ◽  
The Individual

In bottom-up, label-free discovery proteomics, biological samples are acquired in a data-dependent (DDA) or data-independent (DIA) manner, with peptide signals recorded in an intact (MS1) and fragmented (MS2) form. While DDA has only the MS1 space for quantification, DIA contains both MS1 and MS2 at high quantitative quality. DIA profiles of complex biological matrices such as tissues or cells can contain quantitative interferences, and the interferences at the MS1 and the MS2 signals are often independent. When comparing biological conditions, the interferences can compromise the detection of differential peptide or protein abundance and lead to false positive or false negative conclusions.We hypothesized that the combined use of MS1 and MS2 quantitative signals could improve our ability to detect differentially abundant proteins. Therefore, we developed a statistical procedure incorporating both MS1 and MS2 quantitative information of DIA. We benchmarked the performance of the MS1-MS2-combined method to the individual use of MS1 or MS2 in DIA using four previously published controlled mixtures, as well as in two previously unpublished controlled mixtures. In the majority of the comparisons, the combined method outperformed the individual use of MS1 or MS2. This was particularly true for comparisons with low fold changes, few replicates, and situations where MS1 and MS2 were of similar quality. When applied to a previously unpublished investigation of lung cancer, the MS1-MS2-combined method increased the coverage of known activated pathways.Since recent technological developments continue to increase the quality of MS1 signals (e.g. using the BoxCar scan mode for Orbitrap instruments), the combination of the MS1 and MS2 information has a high potential for future statistical analysis of DIA data.

Improved Alignment Technique for Dish Concentrators

Solar Energy ◽  
2003 ◽  
Author(s):  
Charles E. Andraka ◽  
Richard B. Diver ◽  
K. Scott Rawlinson
Keyword(s):  
Focal Length ◽  
Electric Energy ◽  
Parabolic Systems ◽  
Companion Paper ◽  
Quantitative Information ◽  
Key Factors ◽  
Look Back ◽  
Parabolic Dish ◽  
The Individual

Parabolic dish concentrators have shown significant promise of generating competitive electric energy for grid and off-grid applications. The efficiency of a dish-electric system is strongly affected by the quality of the concentrator optics. Most parabolic systems consist of a number of facets mounted to a support structure in an approximate parabolic arrangement, where the individual facets have spherical or parabolic optical shapes. The individual facets must be accurately aligned because improper alignment can compromise performance or create hot spots that can reduce receiver life. A number of techniques have been used over the years to align concentrator facets. In the Advanced Dish Development System (ADDS) project, a color look-back alignment approach that accurately aligns facets (mirror panels) and in addition indicates quantitative information about the focal length was developed. Key factors influencing the alignment, some of which had very large effects on the quality of the alignment, were also identified. The influence of some of the key factors was characterized with a flux mapping system on the second-generation ADDS concentrator. Some of these factors also affect other alignment approaches. The approach was also successfully applied to two other concentrators with differing facet arrangements. Finally, we have extended the method to a 2-f approach that eliminates the need for a distant line-of-sight to the dish and permits alignment at near vertical dish attitudes. In this paper, we outline the color look-back alignment approach, discuss the key alignment factors and their effect on flux distribution, and discuss extensions to non-gore dishes. A companion paper discusses the 2-f color alignment approach in detail.

scholarly journals The combined use of a fermentoterapiya and carboxytherapy at complications of planimetric plasticity of area of a brush in cosmetology

2020 ◽  
Vol 19 (2) ◽  
pp. 83-89
Author(s):  
I. P. Aksenenko
Keyword(s):  
Clinical Symptoms ◽  
Calcium Hydroxyapatite ◽  
Combined Method ◽  
Age Related ◽  
Combined Use ◽  
Psychoemotional State ◽  
Hand Area ◽  
Combined Technique ◽  
Hydroxyapatite Crystals

Background. In recent years, cosmetology has become increasingly popular procedures for the introduction of various fillers to correct age-related changes in the hands (visualization of veins and tendons) to compensate for the amount of fat lost with age in the back of the hand. At the same time, the number of complications after contouring the hand area is also increasing, due to non-compliance with the insertion technique, ignorance of the anatomical features of the corrected area, and a number of other factors. Aims: to study the effectiveness of a combined method that includes a course of collalizine diadinamophoresis in combination with injection carboxytherapy for the correction of complications that occurred on the back of the hand when injecting fillers based on calcium hydroxyapatite. Methods. There were 28 women under observation, with an average age of 41.5 4.6 years, with a complication after administration of a drug based on calcium hydroxyapatite on the back of the hand. Were allocated to 3 groups that received a course of carboxytherapy, the rate of diadynamophoresis collalizine and their combination. Results. The use of a combined course of collalizine diadinamophoresis with injectable carboxytherapy provides a pronounced analgesic effect, stabilizes the psychoemotional state and gives a pronounced effect of reducing the thickness of the dermis and increasing the dermis density coefficient, especially in the early stages of observation, which is probably due to a decrease in intra-dermal edema that occurs as a reaction to a filler injected either too deeply or in large quantities. In addition, this combined technique significantly accelerates both the biodegradation of the previously introduced filler, and affects pathological fibrosis around calcium hydroxyapatite crystals. Conclusions. The combined use of collazine diadinamophoresis and injective carboxytherapy locally on lesions with complications in the form of edema, contouring and local neuropathic manifestations that occur when injecting fillers based on calcium hydroxyapatite to the back of the hand is a highly effective method of treatment, which is confirmed by relieving clinical symptoms and improving the quality of life of patients.

Digital Literacy for Health

2012 ◽  
pp. 142-152
Author(s):  
Ela Klecun
Keyword(s):  
Health Care ◽  
Health Literacy ◽  
Web 2.0 ◽  
Quality Of Health Care ◽  
Digital Literacy ◽  
The Internet ◽  
Sources Of Information ◽  
Individual Use ◽  
The Individual

This paper outlines and challenges expectations and promises regarding the potential of the internet and Web 2.0 for empowering patients and citizens. It focuses on literacies required to make a meaningful (to the individual) use of these technologies for health and health care related purposes. The author briefly discusses how these should be taught and concludes that these literacies, including digital literacy and health literacy, are complex and challenging to many while the empowering claims are over-stated. Traditional sources of information and advice will remain essential to maintaining quality of health care.

scholarly journals The method of combined use of enzyme therapy and carboxytherapy in the correction of complications after injection of polylactic acid in cosmetology

2019 ◽  
Vol 18 (6) ◽  
pp. 379-385
Author(s):  
Irina P. Aksenenko
Keyword(s):  
Polylactic Acid ◽  
Emotional State ◽  
Clinical Symptoms ◽  
Treatment Method ◽  
Enzyme Therapy ◽  
Combined Method ◽  
Combined Use ◽  
Combined Technique ◽  
Density Coefficient

Objective. To study the effectiveness of the combined method, including the course of colladizine diadynamophoresis 1000 KE (collagenase units) in combination with injection carboxytherapy in the correction of complications that occurred when superficial administration of polylactic acid into the skin. Material and methods. Under observation were 38 women of 32 to 39 years old with a complication after the introduction of a filler based on polylactic acid with localization in the abdomen. Three groups were identified that received a course of carboxytherapy, a course of collagenase diadynamophoresis, and their combination. Results. The use of the combined course of Collizin diadynamophoresis with injection carboxytherapy provides a pronounced analgesic effect, improves microcirculation, stabilizes the psycho-emotional state and gives a pronounced effect of reducing the thickness of the dermis and increasing the density coefficient of the dermis, which is probably associated with a decrease in intradermal edema, which occurs as a reaction to superficially introduced filler. In addition, this combined technique significantly accelerates the biodegradation of the surface-injected filler and affects pathological fibrosis around polylactic acid. Conclusions. The combined use of collizine diadynamophoresis and injection carboxytherapy locally on the lesion sites with complications such as surface administration of a filler based on polylactic acid is a highly effective treatment method, which is confirmed by the relief of clinical symptoms and an increase in the quality of life of patients.

scholarly journals A method of treating complications after contour plasty with preparations based on stabilized hyaluronic acid in the nasolacrimal sulcus region

2021 ◽  
Vol 19 (3) ◽  
pp. 145-151
Author(s):  
I. P. Aksenenko ◽  
M. Yu. Gerasimenko
Keyword(s):  
Quality Of Life ◽  
Hyaluronic Acid ◽  
Clinical Symptoms ◽  
Clinical Manifestations ◽  
Treatment Method ◽  
Combined Method ◽  
Combined Use ◽  
Psychoemotional State ◽  
Density Coefficient

Background.In order to correct involutive changes in the skin or aesthetic defects, fillers based on stabilized hyaluronic acid, which are used to fill in various wrinkles and creases, have recently become most popular. It is the procedure of contouring that is one of the three most popular cosmetic procedures. Objective:to study the effectiveness of a combined method that includes a course of longidase diadinamophoresis and injectable carboxytherapy in correcting complications of contouring the nasolacrimal sulcus area with fillers based on stabilized hyaluronic acid. Methods.There were 29 women under observation, average age 36.4 4.4 years with complications after the introduction of a filler based on stabilized hyaluronic acid with localization in the nasolacrimal sulcus. There were 3 groups of patients who received a course of longidase diadinamophoresis (3000 IU), a course of carboxytherapy, or a combination of them. Results.The use of a combined course of longidase diadinamophoresis (3000 IU) and carboxytherapy has a significant effect, reducing the clinical manifestations in the form of contouring and compaction, local edema and neuropathy, and stabilizing the psychoemotional state of patients. In addition, the treatment complex significantly accelerates the biodegradation of the filler and has a restructuring effect on the skin of the nasolacrimal furrow-reduces the thickness of the dermis and increases the dermis density coefficient, which is probably due to the regression of intra-dermal edema that occurs as a reaction to a superficially introduced filler based on stabilized hyaluronic acid. Conclusion.The combined use of the method, which includes a course of longidase diadinamophoresis and injectable carboxytherapy for the correction of complications of nasolacrimal sulcus contouring with fillers based on stabilized hyaluronic acid in the form of local edema, neuropathy and filler contouring, is a highly effective treatment method, which is confirmed by the relief of clinical symptoms, acceleration of drug biodegradation and improvement of the quality of life of patients.

scholarly journals Application of combined enzyme therapy for complications of lip contouring in cosmetology

2020 ◽  
Vol 19 (4) ◽  
pp. 227-233
Author(s):  
Irina P. Aksenenko
Keyword(s):  
Hyaluronic Acid ◽  
Clinical Effect ◽  
Clinical Symptoms ◽  
Enzyme Therapy ◽  
Combined Method ◽  
Combined Use ◽  
Psychoemotional State ◽  
Combined Enzyme Therapy ◽  
Density Coefficient

Purpose: to study the effectiveness of a combined method that includes a course of magnetolaserophoresis of longidase and diadinamophoresis of collalizin 1000 KE (collagenase units) in correcting complications of lip contouring that occurred when a large volume of filler or surface injection of a drug based on stabilized hyaluronic acid into the skin. Material and methods. We observed 31 women aged 25 to 32 years with complications after the introduction of a filler based on stabilized hyaluronic acid with localization in the lip area. Were allocated to three groups who received a course of magnetolaserotherapy of longidaza, the rate of diadynamophoresis of collalizin, or a combination. Results. The use of a combined course of longidase magnetolaserophoresis with collazine diadinamophoresis has a significant clinical effect, reducing edema, gel contouring phenomena and stabilizing the psychoemotional state of patients. In addition, the treatment complex significantly accelerates the biodegradation of the filler and has a restructuring effect on the lip tissue, gives a pronounced effect of reducing the thickness of the dermis and increasing the density coefficient of the dermis, which is probably due to a decrease in intra-dermal edema that occurs as a reaction to a surface-injected filler. Conclusions. Combined use of magnetolaserotherapy of longidaza and diadynamophoresis of collalizin to the lip when complications in the form of a surface of introduction of the filler or injecting too much of the drug based on stabilized hyaluronic acid is a highly effective method of treatment, which is confirmed by relief of clinical symptoms and improvement in quality of life of patients.

Digital Literacy for Health

2010 ◽  
Vol 1 (3) ◽  
pp. 48-57 ◽  
Author(s):  
Ela Klecun
Keyword(s):  
Health Care ◽  
Health Literacy ◽  
Web 2.0 ◽  
Quality Of Health Care ◽  
Digital Literacy ◽  
The Internet ◽  
Sources Of Information ◽  
Individual Use ◽  
The Individual

This paper outlines and challenges expectations and promises regarding the potential of the internet and Web 2.0 for empowering patients and citizens. It focuses on literacies required to make a meaningful (to the individual) use of these technologies for health and health care related purposes. The author briefly discusses how these should be taught and concludes that these literacies, including digital literacy and health literacy, are complex and challenging to many while the empowering claims are over-stated. Traditional sources of information and advice will remain essential to maintaining quality of health care.

Efficacy of combined use of low-dose isotretinoin and phototherapy in patients with different subtypes of rosacea

2020 ◽  
pp. 53-56
Author(s):  
Yu. I. Matushevskaya ◽  
E. V. Agafonova
Keyword(s):  
Low Dose ◽  
Combined Therapy ◽  
Outpatient Basis ◽  
Light Wavelength ◽  
Combined Method ◽  
Incoherent Light ◽  
Combined Use ◽  
Group 2 ◽  
Group 1

Material and methods. 73 patients with different subtypes of rosacea were under observation on an outpatient basis. To study the effectiveness of the combined method of treatment, all patients, depending on the therapy used by the method of simple randomization, were divided into 2 groups, within the groups into 3 subgroups, depending on the subtype of rosacea. Patients of the 1st group received monotherapy with isotretinoin at a dose of 8 mg per day in a daily regimen for three months, then in a regimen every other day for another 3 months. Patients in group 2 received combined therapy with isotretinoin in combination with phototherapy with broadband incoherent light (wavelength 440–950 nm) once every two weeks, a course of 4–6 procedures. Patients of all groups used specialized dermatological cosmetics throughout the study period.Research results. The developed combined method, regardless of the subtype of rosacea, is more effective than monotherapy with isotretinoin: with ETPR, the total DISHS index decreased by 91.2%; with PPPR by 89.6%; with a combination of subtypes by 93.8% versus 41.6%, 53.4% and 54.0% in group 1, respectively.Conclusion. The developed combined method contributes to a more pronounced improvement in the quality of life compared to monotherapy: the DIKI index in group 2 with ETPR decreased by 70.5%, with PPPD by 88.5%, with a combination of subtypes by 95.0%, while in 1 group by 36.3%, 72.4% and 73.8%, respectively. 

Semi-automated methods for 3-D reconstruction of macromolecular complexes

1995 ◽  
Vol 53 ◽  
pp. 42-43
Author(s):  
B. Carragher ◽  
M. Whittaker
Keyword(s):  
Three Dimensional ◽  
Time Saving ◽  
Macromolecular Complexes ◽  
Symmetry Properties ◽  
Dimensional Reconstruction ◽  
Experienced Operator ◽  
Projection Images ◽  
The Individual ◽  
Natural Symmetry

Techniques for three-dimensional reconstruction of macromolecular complexes from electron micrographs have been successfully used for many years. These include methods which take advantage of the natural symmetry properties of the structure (for example helical or icosahedral) as well as those that use single axis or other tilting geometries to reconstruct from a set of projection images. These techniques have traditionally relied on a very experienced operator to manually perform the often numerous and time consuming steps required to obtain the final reconstruction. While the guidance and oversight of an experienced and critical operator will always be an essential component of these techniques, recent advances in computer technology, microprocessor controlled microscopes and the availability of high quality CCD cameras have provided the means to automate many of the individual steps.During the acquisition of data automation provides benefits not only in terms of convenience and time saving but also in circumstances where manual procedures limit the quality of the final reconstruction.

Manageable Self in the Early Hellenistic Era

2010 ◽  
Vol 39 (2) ◽  
pp. 34-36
Author(s):  
Vaia Touna
Keyword(s):  
Social Economic ◽  
Ancient Greece ◽  
The Self ◽  
Hellenistic Period ◽  
Political Conditions ◽  
The Individual

This paper argues that the rise of what is commonly termed "personal religion" during the Classic-Hellenistic period is not the result of an inner need or even quality of the self, as often argued by those who see in ancient Greece foreshadowing of Christianity, but rather was the result of social, economic, and political conditions that made it possible for Hellenistic Greeks to redefine the perception of the individual and its relationship to others.

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