Topology and membrane interaction of Helicobacter pylori ComB proteins involved in natural transformation competence

2003 ◽  
Vol 293 (2-3) ◽  
pp. 153-165 ◽  
Author(s):  
Dirk Hofreuter ◽  
Arno Karnholz ◽  
Rainer Haas
Keyword(s):  
Helicobacter Pylori ◽  
Natural Transformation ◽  
Membrane Interaction ◽  
Transformation Competence ◽  
Natural Transformation Competence

scholarly journals Characteristics of Helicobacter pylori natural transformation

2000 ◽  
Vol 186 (2) ◽  
pp. 275-280 ◽  
Author(s):  
Dawn A Israel ◽  
Angela S Lou ◽  
Martin J Blaser
Keyword(s):  
Helicobacter Pylori ◽  
Natural Transformation

scholarly journals comH, a Novel Gene Essential for Natural Transformation of Helicobacter pylori

2000 ◽  
Vol 182 (14) ◽  
pp. 3948-3954 ◽  
Author(s):  
Leonard C. Smeets ◽  
Jetta J. E. Bijlsma ◽  
Sacha Y. Boomkens ◽  
Christina M. J. E. Vandenbroucke-Grauls ◽  
Johannes G. Kusters
Keyword(s):  
Helicobacter Pylori ◽  
Natural Transformation ◽  
Bacterial Genome ◽  
Uptake System ◽  
Dna Uptake ◽  
Mutant Library ◽  
Chromosomal Dna ◽  
Orthologous Genes ◽  
Helicobacter Felis ◽  
H Pylori

ABSTRACT Helicobacter pylori is naturally competent for transformation, but the DNA uptake system of this bacterium is only partially characterized, and nothing is known about the regulation of competence in H. pylori. To identify other components involved in transformation or competence regulation in this species, we screened a mutant library for competence-deficient mutants. This resulted in the identification of a novel,Helicobacter-specific competence gene (comH) whose function is essential for transformation of H. pyloriwith chromosomal DNA fragments as well as with plasmids. Complementation of comH mutants in transcompletely restored competence. Unlike other transformation genes ofH. pylori, comH does not belong to a known family of orthologous genes. Moreover, no significant homologs ofcomH were identified in currently available databases of bacterial genome sequences. The comH gene codes for a protein with an N-terminal leader sequence and is present in both highly competent and less-efficient transforming H. pyloristrains. A comH homolog was found in Helicobacter acinonychis but not in Helicobacter felis andHelicobacter mustelae.

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