Isotope dilution models for partitioning amino acid uptake by the liver, mammary gland and hindlimb tissues of ruminants.

Author(s):  
L. A. Crompton ◽  
J. France ◽  
B. J. Bequette ◽  
J. A. Maas ◽  
M. D. Hanigan ◽  
...  
Neonatology ◽  
1985 ◽  
Vol 48 (4) ◽  
pp. 250-256 ◽  
Author(s):  
Juan R. Viña ◽  
Inmaculada R. Puertes ◽  
Juan B. Montoro ◽  
Guillermo T. Saez ◽  
José Viña

1985 ◽  
Vol 13 (5) ◽  
pp. 876-877 ◽  
Author(s):  
JUAN R. VIÑA ◽  
ARGIMIRO RODRIGUEZ ◽  
JUAN B. MONTORO ◽  
ANTONIO IRADI ◽  
INMACULADA R. PUERTES ◽  
...  

1997 ◽  
Vol 75 (5) ◽  
pp. 1266 ◽  
Author(s):  
N L Trottier ◽  
C F Shipley ◽  
R A Easter

1981 ◽  
Vol 9 (5) ◽  
pp. 392-392 ◽  
Author(s):  
JUAN R. VIÑA ◽  
JUAN B. MONTORO ◽  
INMACULADA R. PUERTES ◽  
JOSE VIÑA

1976 ◽  
Vol 158 (2) ◽  
pp. 355-359 ◽  
Author(s):  
L D Anderson ◽  
J A Rillema

The effects of insulin, cortisol and prolactin on amino acid uptake and protein biosynthesis were determined in mammary-gland explants from mid-pregnant mice. Insulin stimulated [3H]leucine incorporation into protein within 15 min of adding insulin to the incubation medium. Insulin also had a rapid stimulatory effect on the rate of aminoiso[14C]butyric acid uptake, but it had no effect on the intracellular accumulation of [3H]leucine. Cortisol inhibited the rate of [3H]leucine incorporation into protein during the initial 4h of incubation, but it had no effect at subsequent times. [3H]Leucine uptake was unaffected by cortisol, but amino[14C]isobutyric acid uptake was inhibited after a 4h exposure period to this hormone. Prolactin stimulated the rate of [3H]leucine incorporation into protein when tissues were exposed to this hormone for 4h or more; up to 4h, however, no effect of prolactin was detected. At all times tested, prolactin had no effect on the uptake of either amino[14C]isobutyric acid or [3H]leucine. Incubation with actinomycin D abolished the prolactin stimulation of protein biosynthesis, but this antibiotic did not affect the insulin response. A distinct difference in the mechanism of action of these hormones on protein biosynthesis in the mammary gland is thus apparent.


1979 ◽  
Vol 46 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Andrew R. Peters ◽  
Stephen Alexandrov ◽  
T. Ben Mepham

SUMMARYThe effects of high rates of infusion of essential amino acids on amino acid uptake by the isolated perfused guinea-pig mammary gland were studied. Infusion of methionine, tyrosine, phenylalanine, histidine and tryptophan (designated group 1) resulted in significant increases in the uptakes of tyrosine, phenylalanine and histidine. Methionine, tryptophan and other essential amino acids were not significantly affected. Infusion of threonine, valine, isoleucine, leucine, lysine and arginine (designated group 2) resulted in significant increases in uptake of all these amino acids. Group 1 amino acid uptake was not significantly affected. Infusion of all the essential amino acids (i.e. groups 1 and 2 together) resulted in significant increases in all their uptakes. Using as index ‘the predicted rate of protein synthesis’, infusion of group 1 and 2 together led to an apparent 27% increase in protein synthesis. The above results are discussed in relation to the control of milk protein synthesis by limiting essential amino acids.


1986 ◽  
Vol 14 (2) ◽  
pp. 311-312 ◽  
Author(s):  
JUAN R. VIÑA ◽  
INMACULADA R. PUERTES ◽  
JUAN B. MONTORO ◽  
ARGIMIRO RODRIGUEZ ◽  
JOSÉ VIÑA

1991 ◽  
Vol 74 (10) ◽  
pp. 3412-3420 ◽  
Author(s):  
J.A. Metcalf ◽  
J.D. Sutton ◽  
J.E. Cockburn ◽  
D.J. Napper ◽  
D.E. Beever

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