Molecular tools for fast identification of resistance and characterization of MDR/XDR-TB.

2013 ◽  
pp. 125-136
Author(s):  
S. Cadmus ◽  
D. van Soolingen
Keyword(s):  
Molecular Tools ◽  
Fast Identification

scholarly journals Prevalence and Molecular Epidemiological Data onDirofilaria immitisin Dogs from Northeastern States of India

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Sonjoy Kumar Borthakur ◽  
Dilip Kumar Deka ◽  
Saidul Islam ◽  
Dilip Kumar Sarma ◽  
Prabhat Chandra Sarmah
Keyword(s):  
Epidemiological Data ◽  
Elisa Test ◽  
Molecular Techniques ◽  
Diagnostic Methods ◽  
Molecular Tools ◽  
Stray Dogs ◽  
Concentration Technique ◽  
Working Dogs ◽  
Polymerase Chain

The aim of the present study was to determine the prevalence ofDirofilaria immitisin stray, pet, and working dogs (n=413, 266, and 103, resp.) from Guwahati (Assam) and Aizawl (Mizoram), areas located in two Northeastern States of India. Diagnostic methods applied were microscopy (wet film and Knott’s concentration technique), immunological test (Ag ELISA by SNAP 4Dx ELISA kit), and molecular tools (polymerase chain reaction and sequencing), which evidenced 11.38, 18.03, and 13.93% of positive animals, respectively. No significant differences were observed by area (18.23% versus 17.68%) nor by sex (18.1% versus 17.9%), whereas stray dogs proved more infected than other groups (P<0.05). ELISA test evidenced an overall 22.69% of occult infections, mainly in working dogs (60%), and molecular techniques detectedDirofilaria (Nochtiella) repensin 4 stray dogs from Guwahati. Characterization ofD. immitisisolates for ITS-2 region showed close identity with South Asian isolates.

scholarly journals A Viral Ecogenomics Framework To Uncover the Secrets of Nature’s “Microbe Whisperers”

mSystems ◽  
2019 ◽  
Vol 4 (3) ◽  
Author(s):  
Simon Roux
Keyword(s):  
Cell Metabolism ◽  
Ecosystem Functions ◽  
Biological Knowledge ◽  
Viral Sequence ◽  
Molecular Tools ◽  
Microbial Cells ◽  
Health And Disease ◽  
Virus Genomes ◽  
Do So

ABSTRACTMicrobes drive critical ecosystem functions and affect global nutrient cycling along with human health and disease. They do so under strong constraints exerted by viruses, which shape microbial communities’ structure and shift host cell metabolism during infection. While the majority of viruses and their associated impacts remain poorly characterized, a number of mechanisms by which viruses alter microbial cells and ecosystems have already been revealed. Here I outline how a comprehensive host-resolved mapping of viral sequence space will enable a thorough characterization of virus-encoded mechanisms for microbial manipulation. With soon-to-be millions of virus genomes obtained from metagenomes, one of the major challenges resides in the development of methods for high-throughput and high-resolution virus-host pairing, before multi-omics approaches can be leveraged to fully decipher virus-host dynamics in nature. Beyond novel fundamental biological knowledge, these studies will likely provide new molecular tools enabling a precise engineering of microbial cells and communities.

scholarly journals Genetic dissection of drug resistance in trypanosomes

Parasitology ◽  
2013 ◽  
Vol 140 (12) ◽  
pp. 1478-1491 ◽  
Author(s):  
SAM ALSFORD ◽  
JOHN M. KELLY ◽  
NICOLA BAKER ◽  
DAVID HORN
Keyword(s):  
Neglected Tropical Diseases ◽  
Sub Saharan Africa ◽  
Genetic Dissection ◽  
Molecular Tools ◽  
Sequencing Technology ◽  
Exponential Increase ◽  
Technological Developments ◽  
Sub Saharan ◽  
Identification And Characterization

SUMMARYThe trypanosomes cause two neglected tropical diseases, Chagas disease in the Americas and African trypanosomiasis in sub-Saharan Africa. Over recent years a raft of molecular tools have been developed enabling the genetic dissection of many aspects of trypanosome biology, including the mechanisms underlying resistance to some of the current clinical and veterinary drugs. This has led to the identification and characterization of key resistance determinants, including transporters for the anti-Trypanosoma bruceidrugs, melarsoprol, pentamidine and eflornithine, and the activator of nifurtimox-benznidazole, the anti-Trypanosoma cruzidrugs. More recently, advances in sequencing technology, combined with the development of RNA interference libraries in the clinically relevant bloodstream form ofT. bruceihave led to an exponential increase in the number of proteins known to interact either directly or indirectly with the anti-trypanosomal drugs. In this review, we discuss these findings and the technological developments that are set to further revolutionise our understanding of drug-trypanosome interactions. The new knowledge gained should inform the development of novel interventions against the devastating diseases caused by these parasites.

scholarly journals Molecular Tools for the Detection and Characterization of Bacterial Infections: A Review

2008 ◽  
Vol 39 (7) ◽  
pp. 430-436 ◽  
Author(s):  
Alagarraju Muthukumar ◽  
Nicole L. Zitterkopf ◽  
Deborah Payne
Keyword(s):  
Bacterial Infections ◽  
Molecular Tools

Distribution and molecular characterization of Malassezia species isolated from students with pityriasis versicolor in Haiphong City, 2016 - 2017

2021 ◽  
Vol 31 (4) ◽  
pp. 37-42
Author(s):  
Vo Thi Thanh Hien ◽  
Do Ngoc Anh
Keyword(s):  
Secondary School ◽  
Secondary School Students ◽  
Agar Medium ◽  
Pityriasis Versicolor ◽  
Molecular Tools ◽  
School Students ◽  
Malassezia Species ◽  
Pcr Rflp ◽  
Back Face

This research aims to fnd out the Malassezia species in secondary school students with pityriasis versicolor in Hai Phong City, Vietnam. A total of 110 Malassezia fungal strains from 109 students were isolated from pityriasis versicolor patients in four secondary schools in Hai Phong City from August 2016 to December 2017. These samples were cultured on mDixon agar medium containing antibiotic at 32oC for 7 days. All Malassezia isolates were correctly identifed to the species level by CHROMagarTM Malassezia medium and molecular tools (PCR-RFLP and D1/D2 region sequencing). The results showed that M. furfur and M. japonica are two species that caused pityriasis versicolor at the rate of 96.4% and 3.6%, respectively. Most of these Malassezia isolates were isolated in the back, face, and neck. M. furfur was discovered in both areas, while M. japonica was found only in the urban area. M. furfur and M. japonica were two Malassezia species causing pityriasis versicolor in secondary school students in Hai Phong City of Northern Vietnam, in which M. furfur was more common than M. japonica, whereas other Malassezia species were not detected.

scholarly journals Molecular Detection and Characterization of Goat Isolate ofTaenia hydatigenain Turkey

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Armagan Erdem Utuk ◽  
Fatma Cigdem Piskin
Keyword(s):  
Ribosomal Rna ◽  
Molecular Detection ◽  
Sequence Data ◽  
Pcr Amplification ◽  
Small Subunit ◽  
Molecular Tools ◽  
Taenia Hydatigena ◽  
Nucleotide Sequence Data ◽  
First Time

The aim of this study was to provide molecular detection and characterization of the goat isolate ofTaenia hydatigenafrom Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified asTaeniaspp., and partial sequence of mt-CO1 gene was corresponding toT. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate ofT. hydatigenawas submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization ofT. hydatigenawas done for the first time in Turkey.

Re-characterization of hammerhead ribozymes as molecular tools for intermolecular RNA cleavage

2017 ◽  
Vol 15 (21) ◽  
pp. 4681-4685 ◽  
Author(s):  
Getong Liu ◽  
Xin Huang ◽  
Qinlin Pu ◽  
Yongyun Zhao ◽  
Feng Du ◽  
...  
Keyword(s):  
Rna Cleavage ◽  
Molecular Tools ◽  
Hammerhead Ribozymes

A new guideline for the use of hammerhead ribozymes as molecular tools in intermolecular RNA-cleaving.

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