scholarly journals A comparison of the availability and ileal digestibility of lysine in cottonseed and soya-bean meals for grower/finisher pigs

1990 ◽  
Vol 64 (3) ◽  
pp. 663-677 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen ◽  
D. R. Baigent ◽  
R. E. Darnell ◽  
M. R. Taverner

The availability of lysine and the ileal digestibility of amino acids in three cottonseed meals and a soya-bean meal for grower/finisher pigs were determined. The usefulness of the availability estimates for formulating diets was assessed. The availability of lysine, as assessed with a slope-ratio assay, was (proportion of total): cottonseed meal no. 1, 0.27; no. 2, 0.30, no. 3, 0.29; soya-bean meal, 0.90. Heal digestibility of lysine in the meals (proportion of total) was: cottonseed meal no. 1, 0.58; no. 2, 0.68; no. 3, 0.72; soya-bean meal, 0.89. Pigs given diets formulated to the same available lysine concentration grew at similar rates and retained the same amount of lysine in the carcasses. The results indicate that, for meals of high availability (soya-bean meal), reduced ileal digestibility appears to be the main reason for reduced availability. However, in meals of low availability (cottonseed meal), reduced ileal digestibility only accounts for part of the reduced availability. Thus, the ileal digestibility of lysine is not a reliable indicator of lysine availability.

1994 ◽  
Vol 71 (4) ◽  
pp. 531-541 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen

Two experiments were conducted to determine the utilization of ileal digestible isoleucine by growing pigs. In the first, the apparent ileal digestibility of amino acids in cottonseed meal, lupin-seed meal and soya-bean meal was determined in pigs fitted with‘T‘-shaped cannulas. In the second, three isoleucine-deficient diets were formulated to 0.23 g ileal digestible isoleucine/MJ digestible energy (DE) with the three protein concentrates contributing the only source of isoleucine in sucrose-based diets. An additional three diets were formulated with supplements of isoleucine to confirm that isoleucine was limiting in the first three diets. The growth performance and retention of isoleucine by pigs given the six diets over the 20–45 kg growth phase were then determined. The apparent ileal digestibility of isoleucine in the three protein concentrates (proportion of total) was: cottonseed meal 0.68, lupin-seed meal 0.86, soya-bean meal 0.86. There were no significant differences (P > 0.05) in growth rates (g/d) and crude protein deposition rates (g/d) of the pigs given the three diets formulated to 0.23 g ileal digestible isoleucine/MJ DE: cottonseed meal 590, 84; lupin-seed meal 613, 87; soya-bean meal 594, 91 (SEM 13.0, 2.9) respectively. The response of pigs to the addition of isoleucine confirmed that isoleucine was limiting in these diets. The proportion of ileal digestible isoleucine retained by pigs given the cottonseed meal (0.65) was slightly lower than that retained by pigs given soya-bean meal (0.73; P < 0.05). These results indicate that values for the ileal digestibility of isoleucine in protein concentrates more closely reflect the proportion of isoleucine that can be utilized by the pig than occurs for other amino acids such as lysine, threonine and methionine.


1984 ◽  
Vol 51 (1) ◽  
pp. 85-99 ◽  
Author(s):  
E. S. Batterham ◽  
R. D. Murison ◽  
L. M. Andersen

1. The availability of lysine in nine vegetable-protein concentrates was assessed using the slope-ratio assay for growing pigs and rats. Diets were equalized for crude fibre using solka floc to minimize any possible effects of variation in fibre content on availability estimates.2. The availability of lysine in the nine proteins for pigs, using food conversion efficiency (FCE) on a carcass basis as the criterion of response were (proportion of total): cottonseed meal 0.39, lupin (Lupinus angustifolius) seed meal no. 10.37, no. 20.65, no. 30.54, no. 40.54, field peas (Pisum sativum) 0.93, peanut (groundnut) meal 0.57, soya-bean meal no. 10.98, no. 2 0.89.3. Estimates of available lysine for rats as assessed by the slope-ratio assay using FCE on a carcass basis were in close agreement with the pig estimates for cottonseed meal (0.35) and soya-bean meal no. 1(0.91) and no. 2(0.89), higher for lupin-seed meals (range 0.70–0.94 with a mean of 0.81) and peanut meal (0.76) and lower for field peas (0.76).4. The differences in available lysine were not detected by the chemical Silcock available-lysine test (Roach et al. 1967) or by the direct 1-fluoro-2,4-dinitrobenzene procedure (Carpenter, 1960).


1990 ◽  
Vol 64 (3) ◽  
pp. 679-690 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen ◽  
D. R. Baigent ◽  
S. A. Beech ◽  
R. Elliott

Two experiments were conducted to determine the utilization of ileal digestible lysine by pigs. In the first, the apparent ileal digestibility of amino acids in cottonseed meal, meat-and-bone meal and soya-bean meal was determined in pigs fitted with‘T'-shaped cannulas. In the second experiment, three lysine-deficient diets were formulated to 0.36 g ileal digestible lysine/MJ digestible energy (DE), with lysine contributed from the three protein concentrates as the only source of lysine in sugar-based diets. An additional three diets were formulated with supplements of lysine to verify that lysine was limiting in the first three diets. The growth performance and retention of lysine by pigs given the six diets over the 20–45 kg growth phase were then determined. The apparent ileal digestibility of lysine in the three protein concentrates (proportion of total) was: cottonseed meal 0.74, meat-and-bone meal 0.78, soya-bean meal 0.89. Growth rates (g/d) of the pigs given the three diets formulated to 0.36 g ileal digestible lysine/MJ DE were significantly different (P < 0.001): cottonseed meal 377, meat-and-bone meal 492, soya-bean meal 541. The response of pigs to the addition of lysine confirmed that lysine was limiting in these diets. Crude protein (nitrogen× 6.25) deposited by the pigs was significantly higher (P < 0.001) for those given soya-bean meal (77 g/d), relative to meat-and-bone meal (66 g/d) and cottonseed meal (38 g/d). The proportion of ileal digestible lysine retained by pigs given the three protein concentrates was: cottonseed meal 0.36, meat-and-bone meal 0.60, soya-bean meal 0.75. The results indicate that values for the ileal digestibility of lysine in protein concentrates are unsuitable in dietary formulations as the assay does not reflect the proportion of lysine that can be utilized by the pig. It appears that, with heat-processed meals, a considerable proportion of the lysine is absorbed in a form(s) that is (are) inefficiently utilized.


2014 ◽  
Vol 99 (4) ◽  
pp. 728-736 ◽  
Author(s):  
A. Aguilera ◽  
T. C. Reis de Souza ◽  
G. Mariscal-Landín ◽  
K. Escobar ◽  
S. Montaño ◽  
...  

1994 ◽  
Vol 71 (3) ◽  
pp. 345-360 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen ◽  
D. R. Baigent

The ileal digestibility of tryptophan for growing pigs was determined for cottonseed, meat-and-bone and soya-bean meals. Tryptophan in the food and digesta was measured by two analytical procedures (NaOH hydrolysis and colorimetric estimation (method 1) and LiOH hydrolysis and HPLC determination (method 2)). The results were respectively: cottonseed meal 0.46, 0.81; meat-and-bone meal 0.55, 0.65; soya-bean meal 0.74, 0.90. In the first experiment the values for method 1 were shown to be inapplicable to pigs. In a second experiment three tryptophan-deficient diets (005 g ileal digestible tryptophan/MJ digestible energy (DE)) were formulated using values from method 2 for cottonseed meal, meat-and-bone meal plus L-tryptophan and soya-bean meal respectively as the only sources of tryptophan in the diets. This experiment was terminated after 28 d as overall growth performance of the pigs was very low. A third experiment was conducted in a similar manner to Expt 2 except that the diets were formulated to 0.065g ileal digestible tryptophan/MJ DE and growth responses and tryptophan retention were assessed over the 20–45 kg growth phase. Growth rates (g.d) of the pigs given the three diets were significantly different (P < 0.01): cottonseed meal 393, meat-and-bone meal plus L-tryptophan 531, soya-bean meal 437 (SED 39.0). Tryptophan retention (as a proportion of ileal digestible tryptophan intake) was significantly different (P < 0.05): cottonseed meal 0.51, meat-and-bone meal plus L-tryptophan 0.49, soya-bean meal 0.41. These results indicate (1) that the colorimetric technique for assessing tryptophan was inapplicable and (2) that ileal digestible values for tryptophan were not suitable for formulating diets containing heat-processed proteins, possibly due to absorption of some of the tryptophan in a form that was non-utilizable, and/or to underestimation of total tryptophan in the protein concentrates.


1981 ◽  
Vol 46 (3) ◽  
pp. 513-519 ◽  
Author(s):  
E. J. Major ◽  
E. S. Batterham

1. A slope-ratio assay was developed to determine the availability of lysine in protein concentrates for chicks, Two protein concentrates were assessed per assay, using three levels of incorporation into the basal diet.2. Availability of lysine, expressed as a proportion of total lysine in five protein concentrates was: cottonseed meal 0·83, fish meal 1·00, meat-and-bone meal 0·86, soya-bean meal 0·93, sunflower meal 1·01.3. The five protein concentrates had previously been assayed for available lysine with slope-ratio assays for pies and rats (Batterham et al. 1979; Batterharn et al. 1981), There was little relationship between the results for chicks and those for pigs and rats. For pigs, availability estimates ranged from 0·43 for cottonseed meal to 0·89 for fish meal. For rats, availability estimates ranged from 0·49 for sunflower meal to 1·04 for fish meal.4. The results for chicks were in closer agreement with values obtained using the Silcock available-lysine assay (Roach et al. 1967) and the direct 1-fluoro-2,4-dinitrobenzene procedure (Carpenter, 1960). By contrast, there was litlle relationship between the chemical tests and results for pigs or rats.


1993 ◽  
Vol 70 (3) ◽  
pp. 711-720 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen ◽  
D. R. Baigent

An experiment was conducted to determine the utilization of ileal digestible methionine by growing pigs. Three methionine-deficient diets (0·09 g ileal digestible methionine/MJ digestible energy (DE)) were formulated using cottonseed meal, meat-and-bone meal and soya-bean meal respectively as the only source of methionine in the diet. An additional three diets were formulated with supplements of methionine to confirm that methionine was limiting in the first three diets. The growth performance and retention of methionine by pigs given the six diets over the 20–45 kg growth phase was then determined. Growth rates (g/d) of pigs given the three diets formulated to 0·09 g ileal digestible methionine/MJ DE were significantly different (P < 0·01): cottonseed meal 411, meat-and-bone meal 442, soya-bean meal 496 (SED 24·6). The response of pigs to the addition of methionine confirmed that methionine was limiting in these diets. Crude protein (N × 6·25) deposited by the pigs (g/d) was significantly higher (P < 0·05) for those given soya-bean meat (61) and meat-and-bone meal (57) relative io cottonseed meal (47; SED 3·3). The proportion of ileal digestible methionine retained by pigs given the three protein concentrates was: cottonseed meal 0·39, meat-and-bone meal 0·45, soya-bean meal 0·47 (SED 0·019). These results indicate that values for the ileal digestibility of methionine in protein concentrates do not reflect the proportion of methionine that can be utilized by the pig. It appears that, with heat-processed meals, a considerable proportion of the methionine is absorbed in a form(s) that is (are) inefficiently utilized.


1996 ◽  
Vol 5 (6) ◽  
pp. 547-555 ◽  
Author(s):  
Markku Mäkinen ◽  
Hilkka Siljander-Rasi

A study was conducted on four castrated male pigs to determine the apparent ileal digestibility (AID) of crude protein (CP) and amino acids in wheat protein (WP), a wet by-product of the starch and gluten industry, and in soya bean meal (SBM). The pigs were fitted with a steered ileo-caecal valve cannula at a liveweight of 35 kg. They were assigned to two semi-purified wheat starch based diets, with either WP or SBM as a sole protein source, and fed according to a three-period reversal design. The diets were formulated to contain 140 g CP/kg DM, 11.3 MJ net energy/kg DM and similar amounts of lysine, methionine and threonine. The CP content and the lysine and threonine contents in CP were lower in WP than in SBM. Nearly half of the DM in WP was starch, and the crude fibre content of the product was very low. The apparent ileal and total tract digestibilities of CP were very similar in both the WP and SBM diets. The AID of methionine was higher in WP (88.2%) than in SBM (84.6%) diets (P


2009 ◽  
Vol 150 (3-4) ◽  
pp. 238-248 ◽  
Author(s):  
D.G. Valencia ◽  
M.P. Serrano ◽  
R. Lázaro ◽  
E. Jiménez-Moreno ◽  
G.G. Mateos

1965 ◽  
Vol 7 (3) ◽  
pp. 303-308 ◽  
Author(s):  
H. B. Bowers ◽  
T. R. Preston ◽  
N. A. MacLeod ◽  
I. McDonald ◽  
Euphemia B. Philip

SUMMARY1. Nitrogen balance studies were made on 8 Friesian steers given allconcentrate diets containing fish meal, soya bean meal, groundnut meal or cottonseed meal.2. There were no differences in nitrogen retention but voluntary feed intake was significantly lower on the fish meal diet.3. Adjustment of nitrogen retention to constant dry matter intake resulted in significantly higher values for the diets containing fish meal and cottonseed meal than for the others.4. Rumen pH, rumen ammonia and blood urea were all much lower on the fish meal diet.


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