Development and Validation of High Performance Liquid Chromatographic and UV-Derivative Spectrophotometric Methods for the Determination of Sotalol Hydrochloride in Tablets

Abstract High-performance liquid chromatographic (LC) and ultraviolet derivative spectrophotometric (UVD) methods were developed and validated for the quantitative determination of epinastine hydrochloride in coated tablets. LC was performed on a reversed-phase RP-18 column with a mobile phase composed of 0.3 triethylamine (pH adjusted to 4.0 with 10 orthophosphoric acid)methanol (60 + 40, v/v). The first-order derivative method was performed at 243.8 nm using HCl and methanol as the solvent. The methods were validated according to U.S. Pharmacopoeia and International Conference on Harmonization guidelines. The statistical analysis by Student's t-test showed no significant difference between the results obtained by the 2 methods. The proposed methods were found to be simple, rapid, precise, accurate, robust, and sensitive, allowing perfect interchange. The LC and UVD methods can be used in the routine quantitative determination of the epinastine hydrochloride in coated tablets.

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Development and Validation of a HPLC and an UV Spectrophotometric Methods for Determination of Dexibuprofen in Pharmaceutical Preparations

ISRN Pharmaceutics ◽

10.5402/2011/948314 ◽

2011 ◽

Vol 2011 ◽

pp. 1-4

Author(s):

Selvadurai Muralidharan ◽

Subramania Nainar Meyyanathan

Keyword(s):

High Performance ◽

Pharmaceutical Preparations ◽

High Performance Liquid Chromatographic ◽

Reversed Phase ◽

Pharmaceutical Dosage Form ◽

Spectrophotometric Methods ◽

Validation Parameters ◽

Development And Validation ◽

Liquid Chromatographic

A high-performance liquid chromatographic (HPLC) and a ultraviolet (UV) methods were developed and validated for the quantitative determination of Dexibuprofen (DI) in pharmaceutical dosage form. HPLC was carried out by reversed phase technique on a RP-18 column with a mobile phase composed of acetonitrile and 0.5% triethylamine (pH 7.5 adjusted with orthophosphoric acid (30 : 70, v/v)). UV method was performed with the λ max at 222.0 nm. Both the methods showed good linearity, reproducibility and precision. No spectral or chromatographic interferences from the tablet excipients were found in UV and HPLC. The method was successfully applied to commercial DEXIFEN tablets. Validation parameters such as linearity, precision, accuracy, and specificity were determined. The proposed method could be applicable for routine analysis of DI and monitoring of the quality of marketed drugs.

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Development and validation of a non-aqueous reversed-phase high-performance liquid chromatographic method for the determination of four chemical UV filters in suncare formulations

Journal of Chromatography A ◽

10.1016/j.chroma.2003.08.078 ◽

2004 ◽

Vol 1031 (1-2) ◽

pp. 319-324 ◽

Cited By ~ 22

Author(s):

C.G Smyrniotakis ◽

Helen A Archontaki

Keyword(s):

High Performance ◽

Chromatographic Method ◽

High Performance Liquid Chromatographic ◽

Reversed Phase ◽

Uv Filters ◽

Liquid Chromatographic Method ◽

Development And Validation ◽

Liquid Chromatographic

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Method Development and Validation of Propofol by Reverse Phase HPLC and its Estimation in Commercial Formulation

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00547 ◽

2021 ◽

pp. 3139-3142

Author(s):

Kuntal Mukherjee ◽

S. T. Narenderan ◽

B. Babu ◽

Survi Mishra ◽

S. N. Meyyanathan

Keyword(s):

High Performance ◽

Method Development ◽

Pharmaceutical Formulations ◽

High Performance Liquid Chromatographic ◽

Reverse Phase Hplc ◽

Liquid Chromatographic Method ◽

Method Development And Validation ◽

Development And Validation ◽

Liquid Chromatographic

A simple, sensitive and rapid high performance liquid chromatographic method has been developed for the determination of Propofol. The main focus of the method was to determine Propofol in solution form as well as in marketed formulation. Chromatographic separation was achieved on Inertsil ODS-3V column (250mm x 4.6mm; 5µm) with a mobile phase consisting of methanol: water (85:15), with a flow rate of 1.0ml/min (UV detection at 270nm). Linearity was observed over the concentration range of 10-110µg/ml with a regression equation y=88048x + 44524 and having a regression value (R2) of 0.999. The LOD and LOQ values found to be 10ng and 100ng, respectively. No changes found in ruggedness and robustness studies. The percentage of recovery was found to be 95.25% to 101.81%. Validation studies revealed that the method was specific, accurate, precise, reliable, robust, reproducible and suitable for the quantitative analysis in its pharmaceutical formulations.

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DEVELOPMENT AND VALIDATION OF A NEW RP HPLC METHOD FOR ROUTINE DETERMINATION OF TEA TREE OIL IN BULK AND COSMECEUTICAL FORMULATIONS

INDIAN DRUGS ◽

10.53879/id.56.07.11802 ◽

2019 ◽

Vol 56 (07) ◽

pp. 43-49

Author(s):

B.P. Manjula ◽

V. G Joshi ◽

Siddamsetty Ramachandra Setty ◽

M Geetha ◽

Keyword(s):

High Performance ◽

High Performance Liquid Chromatographic ◽

Reversed Phase ◽

Hplc Method ◽

Tea Tree Oil ◽

Tea Tree ◽

Liquid Chromatographic Method ◽

Development And Validation ◽

Liquid Chromatographic

Tea tree oil, an active ingredient of skin, hair and nail care cosmeceuticals, has claims for topical antimicrobial, analgesic and anti-inflammatory activity. Its complex composition is governed by ISO 4730:2017. Terpinene-4-ol is the principal constituent of the oil (35% - 48%) followed by γ-terpinene (14% -28%), α-terpinene (6%-12%) and 1,8-cineole (≤15%). A reversed-phase, isocratic high performance liquid chromatographic method has been developed and validated for routine determination of tea tree oil based on1,8-cineole content in bulk and commercially available cosmeceuticals using C18 column, methanol-water (70:30 v/v) as mobile phase and flow rate of 1mL/min. UV detection was done at 200 nm. Linearity of the method was established for 20-100μL/mL (R2 = 0.9992) with LOD, LOQ values of 0.5594 μL/mL and 5.5941μL/mL respectively. The % RSD values for robustness and precision were <1% and recovery ranged between 99.09-102.96%. The method was successfully applied for determination of 1,8-cineole content in cosmeceuticals.

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A rapid spectrophotometric determination of imidacloprid in selected commercial formulations in the presence of 6-chloronicotinic acid

Journal of the Serbian Chemical Society ◽

10.2298/jsc0912455g ◽

2009 ◽

Vol 74 (12) ◽

pp. 1455-1465 ◽

Cited By ~ 6

Author(s):

Valéria Guzsvány ◽

Zsigmond Papp ◽

Sanja Lazic ◽

Ferenc Gaál ◽

Luka Bjelica ◽

...

Keyword(s):

Spectrophotometric Method ◽

High Performance ◽

High Performance Liquid Chromatographic ◽

Model Systems ◽

Zero Crossing ◽

Spectrophotometric Methods ◽

Liquid Chromatographic Method ◽

Relative Standard ◽

Liquid Chromatographic

A simple first-order derivative spectrophotometric method was developed for the simultaneous determination of imidacloprid and 6-chloronicotinic acid (6-CNA). By using the zero-crossing approach, imidacloprid was determined at 249 nm and 6-CNA at 236 nm with detection limits of 0.32 and 0.17 ?g mL-1, respectively, and relative standard deviations not exceeding 1.2 % in the case of model systems. The proposed method was applied for the determination of imidacloprid and 6-CNA in commercial formulations. A conventional spectrophotometric method (at 270 nm) was also employed for the determination of the content of imidacloprid in the same commercial formulations. The results of the developed spectrophotometric methods were in good agreement with those obtained by the high-performance liquid chromatographic method.

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