Hypno-desensitization therapy of vaginismus: Part I. “in vitro” method. Part II. “in vivo” method

1973 ◽  
Vol 21 (3) ◽  
pp. 144-156 ◽  
Author(s):  
K. Fuchs ◽  
Z. Hoch ◽  
E. Paldi ◽  
H. Abramovici ◽  
J. M. Brandes ◽  
...  
Keyword(s):  
In Vitro Method ◽  
Desensitization Therapy

A in Vivo Assay for Standardizing Heparin Preparations

1979 ◽  
Vol 41 (03) ◽  
pp. 576-582
Author(s):  
A R Pomeroy
Keyword(s):  
In Vitro Assays ◽  
British Pharmacopoeia ◽  
In Vitro Method ◽  
Standard Preparation ◽  
Reliable Estimation ◽  
Assay Procedure ◽  
Accuracy And Precision ◽  
Heparin Preparation

SummaryThe limitations of currently used in vitro assays of heparin have demonstrated the need for an in vivo method suitable for routine use.The in vivo method which is described in this paper uses, for each heparin preparation, four groups of five mice which are injected intravenously with heparin according to a “2 and 2 dose assay” procedure. The method is relatively rapid, requiring 3 to 4 hours to test five heparin preparations against a standard preparation of heparin. Levels of accuracy and precision acceptable for the requirements of the British Pharmacopoeia are obtained by combining the results of 3 to 4 assays of a heparin preparation.The similarity of results obtained the in vivo method and the in vitro method of the British Pharmacopoeia for heparin preparations of lung and mucosal origin validates this in vivo method and, conversely, demonstrates that the in vitro method of the British Pharmacopoeia gives a reliable estimation of the in vivo activity of heparin.

scholarly journals In Vitro Effect of Erythropoietin on the Spleen of The Polycythemic Mouse

Blood ◽  
1967 ◽  
Vol 29 (6) ◽  
pp. 852-858
Author(s):  
YASUSADA MIURA ◽  
FUMIMARO TAKAKU ◽  
KIKU NAKAO
Keyword(s):  
Tissue Culture ◽  
Culture Method ◽  
Stem Cell Differentiation ◽  
Mouse Spleen ◽  
In Vitro Method ◽  
Heme Synthesis ◽  
Tissue Culture Method ◽  
Vitro Effect

Abstract 1. An in vitro method to observe radiosensitivity of stem cells was developed in the present study. In vivo and in vitro effect of 60Co irradiation on the erythropoietin-induced stem cell differentiation into erythroblasts was observed, using a tissue culture method of polycythemic mouse spleen. Response to erythropoietin was demonstrated by an appearance of heme synthesis and erythroblasts in spleen fragments. 2. A significant correlation between the rate of appearance of erythroblasts and heme synthesis of the spleen fragments was observed. 3. After irradiation, marked impairment of both heme synthesis and production of erythroblasts was observed, yielding D37 values in the vicinity of 70 r in vivo and 120 r in vitro irradiation, respectively. 4. Marked recovery of erythropoietin-induced heme synthesis in the polycythemic mouse spleen was observed 9 days after 300 r irradiation, with an "overshooting" phenomenon on the 12th day.

scholarly journals Estimation of in vivo digestibility with the laying hen by an in vitro method using the intestinal fluid of the pig

1980 ◽  
Vol 43 (2) ◽  
pp. 389-391 ◽  
Author(s):  
K. Sakamoto ◽  
T. Asano ◽  
S. Furuya ◽  
S. Takahashi
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Laying Hen ◽  
Intestinal Fluid ◽  
Protein Nitrogen ◽  
In Vitro Method ◽  
Poultry Diets

1. Dry matter and crude protein (nitrogen × 6.25) digestibility of four poultry diets determined by an in vitro method using the intestinal fluid of pigs was significantly correlated with corresponding in vivo digestibility values obtained with hens.2. The intestinal fluid could be lyophilized and stored for at least 35 d without losing its activity on digestion.

A novel use of an in vitro method to predict the in vivo stability of block copolymer based nano-containers

2007 ◽  
Vol 122 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Hamidreza Montazeri Aliabadi ◽  
Dion R. Brocks ◽  
Parvin Mahdipoor ◽  
Afsaneh Lavasanifar
Keyword(s):  
Block Copolymer ◽  
In Vitro Method

Detection of reticuloendothelial-depressing substance in shock

1965 ◽  
Vol 209 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Benjamin Blattberg ◽  
Matthew N. Levy
Keyword(s):  
Escherichia Coli ◽  
Superior Mesenteric Artery ◽  
Whole Blood ◽  
Mesenteric Artery ◽  
The Other ◽  
In Vitro Method ◽  
Carbon Particles ◽  
Normal Rat

In an effort to find an in vitro method to detect the presence of reticuloendothelial-depressing substance (RDS), two tests were devised which measured phagocytic activity. One used carbon particles to measure phagocytosis and the other P32-labeled Escherichia coli. Neither method demonstrated an in vitro difference in granulopectic activity between dog plasmas from sham-operated and hemorrhagic-shock or superior mesenteric artery-occluded (SMAO) animals. An in vivo method was used in which the reticuloendothelial activity of the rat was measured in terms of the rate of clearance of injected carbon particles. Occlusion of the superior mesenteric artery of the rat led to the production of RDS. The RDS could be transferred to and demonstrated in a normal rat by means of SMAO rat whole blood, plasma, and dialysate of plasma, but not in RBC or plasma which had been dialyzed. Sham-operated animals were used as controls.

Novel in vitro method to predict in vivo liver-to-plasma unbound partition coefficient

2018 ◽  
Vol 33 (1) ◽  
pp. S91
Author(s):  
Li Di ◽  
Keith Riccardi ◽  
Zhenhong Li ◽  
Jian Lin ◽  
Sangwoo Ryu ◽  
...  
Keyword(s):  
Partition Coefficient ◽  
In Vitro Method

Evaluation of an in vitro method to measure behavioural diapause in the tick Rhipicephalus appendiculatus (Acarina: Ixodidae) in the laboratory

Parasitology ◽  
1997 ◽  
Vol 115 (1) ◽  
pp. 97-100 ◽  
Author(s):  
M. MADDER ◽  
D. L. BERKVENS
Keyword(s):  
Rhipicephalus Appendiculatus ◽  
New Method ◽  
In Vitro Method

A new method has been developed to investigate behavioural diapause in adult Rhipicephalus appendiculatus ticks. It is based on a system of gauze columns in which the activity of the ticks can be monitored in the laboratory. An experiment was conducted, involving different photoperiodic conditions during the nymph-to-adult moulting and pre-questing periods, with the aim of comparing the results obtained by the in vitro method with those of the standard in vivo method. Comparable results were obtained with both methods.

Sign in / Sign up

Export Citation Format

Share Document