Evaluation of an in vitro method to measure behavioural diapause in the tick Rhipicephalus appendiculatus (Acarina: Ixodidae) in the laboratory

Parasitology ◽  
1997 ◽  
Vol 115 (1) ◽  
pp. 97-100 ◽  
Author(s):  
M. MADDER ◽  
D. L. BERKVENS
Keyword(s):  
Rhipicephalus Appendiculatus ◽  
New Method ◽  
In Vitro Method

A new method has been developed to investigate behavioural diapause in adult Rhipicephalus appendiculatus ticks. It is based on a system of gauze columns in which the activity of the ticks can be monitored in the laboratory. An experiment was conducted, involving different photoperiodic conditions during the nymph-to-adult moulting and pre-questing periods, with the aim of comparing the results obtained by the in vitro method with those of the standard in vivo method. Comparable results were obtained with both methods.

A in Vivo Assay for Standardizing Heparin Preparations

1979 ◽  
Vol 41 (03) ◽  
pp. 576-582
Author(s):  
A R Pomeroy
Keyword(s):  
In Vitro Assays ◽  
British Pharmacopoeia ◽  
In Vitro Method ◽  
Standard Preparation ◽  
Reliable Estimation ◽  
Assay Procedure ◽  
Accuracy And Precision ◽  
Heparin Preparation

SummaryThe limitations of currently used in vitro assays of heparin have demonstrated the need for an in vivo method suitable for routine use.The in vivo method which is described in this paper uses, for each heparin preparation, four groups of five mice which are injected intravenously with heparin according to a “2 and 2 dose assay” procedure. The method is relatively rapid, requiring 3 to 4 hours to test five heparin preparations against a standard preparation of heparin. Levels of accuracy and precision acceptable for the requirements of the British Pharmacopoeia are obtained by combining the results of 3 to 4 assays of a heparin preparation.The similarity of results obtained the in vivo method and the in vitro method of the British Pharmacopoeia for heparin preparations of lung and mucosal origin validates this in vivo method and, conversely, demonstrates that the in vitro method of the British Pharmacopoeia gives a reliable estimation of the in vivo activity of heparin.

Hypno-desensitization therapy of vaginismus: Part I. “in vitro” method. Part II. “in vivo” method

1973 ◽  
Vol 21 (3) ◽  
pp. 144-156 ◽  
Author(s):  
K. Fuchs ◽  
Z. Hoch ◽  
E. Paldi ◽  
H. Abramovici ◽  
J. M. Brandes ◽  
...  
Keyword(s):  
In Vitro Method ◽  
Desensitization Therapy

A Model for Non-Invasive Estimation of in Vivo Dialyzer Performances and Patient's Conductivity during Hemodialysis

1993 ◽  
Vol 16 (8) ◽  
pp. 585-591 ◽  
Author(s):  
T. Petitclerc ◽  
N. Goux ◽  
A.L. Reynier ◽  
B. Béné
Keyword(s):  
Strong Correlation ◽  
New Method ◽  
Conductivity Measurements ◽  
Urea Clearance ◽  
Non Invasive ◽  
Model Based ◽  
On Line ◽  
Conventional Methods

On-line monitoring of hemodialysis sessions requires a non-invasive estimation of the parameters concerning the patient's status and the dialyzer performances. We describe here a model based on a new method for non-invasive dialysance and patient conductivity measurements. In this technique the same probe measures alternately the conductivity at the dialysate inlet and outlet for two different dialysate conductivity values. From these data, an appropriate model allows to determine the patient's conductivity as well as the effective dialysance of ionised solutes, that is to say the dialysance corrected for recirculation. A strong correlation is evidenced between the effective dialysance measured by this method and the urea clearance measured by conventional methods (r=0.98 for in vitro solutions; r=0.82 in in vivo situations).

scholarly journals In Vitro Effect of Erythropoietin on the Spleen of The Polycythemic Mouse

Blood ◽  
1967 ◽  
Vol 29 (6) ◽  
pp. 852-858
Author(s):  
YASUSADA MIURA ◽  
FUMIMARO TAKAKU ◽  
KIKU NAKAO
Keyword(s):  
Tissue Culture ◽  
Culture Method ◽  
Stem Cell Differentiation ◽  
Mouse Spleen ◽  
In Vitro Method ◽  
Heme Synthesis ◽  
Tissue Culture Method ◽  
Vitro Effect

Abstract 1. An in vitro method to observe radiosensitivity of stem cells was developed in the present study. In vivo and in vitro effect of 60Co irradiation on the erythropoietin-induced stem cell differentiation into erythroblasts was observed, using a tissue culture method of polycythemic mouse spleen. Response to erythropoietin was demonstrated by an appearance of heme synthesis and erythroblasts in spleen fragments. 2. A significant correlation between the rate of appearance of erythroblasts and heme synthesis of the spleen fragments was observed. 3. After irradiation, marked impairment of both heme synthesis and production of erythroblasts was observed, yielding D37 values in the vicinity of 70 r in vivo and 120 r in vitro irradiation, respectively. 4. Marked recovery of erythropoietin-induced heme synthesis in the polycythemic mouse spleen was observed 9 days after 300 r irradiation, with an "overshooting" phenomenon on the 12th day.

scholarly journals Estimation of in vivo digestibility with the laying hen by an in vitro method using the intestinal fluid of the pig

1980 ◽  
Vol 43 (2) ◽  
pp. 389-391 ◽  
Author(s):  
K. Sakamoto ◽  
T. Asano ◽  
S. Furuya ◽  
S. Takahashi
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Laying Hen ◽  
Intestinal Fluid ◽  
Protein Nitrogen ◽  
In Vitro Method ◽  
Poultry Diets

1. Dry matter and crude protein (nitrogen × 6.25) digestibility of four poultry diets determined by an in vitro method using the intestinal fluid of pigs was significantly correlated with corresponding in vivo digestibility values obtained with hens.2. The intestinal fluid could be lyophilized and stored for at least 35 d without losing its activity on digestion.

A novel use of an in vitro method to predict the in vivo stability of block copolymer based nano-containers

2007 ◽  
Vol 122 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Hamidreza Montazeri Aliabadi ◽  
Dion R. Brocks ◽  
Parvin Mahdipoor ◽  
Afsaneh Lavasanifar
Keyword(s):  
Block Copolymer ◽  
In Vitro Method

Gelatin-Coated Insert-Well Culture Markedly Improved In Vitro Culture of Primary Myeloma Cells.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 5020-5020 ◽  
Author(s):  
Saeid Abroun ◽  
Ken-Ichiro Otsuyama ◽  
Karim Shamsasenjan ◽  
Khademul Islam ◽  
Jaki Amin ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Stromal Cells ◽  
Drug Sensitivity ◽  
Mononuclear Cells ◽  
Synthetic Medium ◽  
New Method ◽  
Phenotypic Data ◽  
Myeloma Cells

Abstract In order to clarify the pathogenesis of multiple myeloma (MM) and identify the molecular target for MM treatment, it is important to understand the biology and molecular mechanism of survival and proliferation of myeloma cells in vitro and in vivo. Still, it is hard to keep primary myeloma cells viable at least for 2 weeks in vitro, and these cells rapidly enter apoptosis even in the presence of IL-6. Co-culture with BM stromal cells is considered to be one of the most important factors as well as addition of cytokines for improvement of in vitro culture of primary myeloma cells. Based on our previous data, we devised a new method where bone marrow mononuclear cells (BMMNC) from BM aspirates were put inside insert-wells (8.0 um pore size, #3182, BD) coated with gelatin in the presence of the mixture of cytokines (galectin-1, SDF-1, IL-6 and IGF-1) in the serum-free synthetic medium; addition of galectin-1 and SDF-1 was essential especially at the beginning of this culture. By this method, we observed that BM stromal cells attached to gelatin and survived well with rather low proliferation; myeloma cells interacted well with these stromal cells. Thus, we could maintain viability of primary myeloma cells at least for 4 weeks and the recovery of viable myeloma cells (CD38++ cell) appeared to be about 80% in 30 cases of MM we examined. Phenotypic data also showed that ratio of immature (MPC-1−) and mature (MPC-1+) myeloma cells in the BMMNC before culture was approximately maintained after 2 or 4 weeks in this method. Therefore, these results suggest that gelatin-coated insert-well culture can control the viability of stromal cells and thus maintain the culture of primary myeloma cells in vitro. This new method would contribute to the further understanding of biology and drug sensitivity of primary myeloma cells.

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