The local time-averaged temperature θs¯ and its fluctuating component θs on the surface of a heated circular cylinder immersed in a cylinder near-wake were measured using a fiber-optic Bragg grating (FBG) sensor. Three cylinder center-to-center spacing, i.e., L/d=5.20, 2.50, and 1.18, were investigated. In order to validate the FBG sensor measurement, a thermocouple and a single hot-wire were employed to measure θs¯ on the heated cylinder and streamwise fluctuating velocity u in the near-wake of the downstream cylinder, respectively. The FBG sensor measurement of θs¯ is in good agreement with that simultaneously obtained by the thermocouple. The measured θs is closely correlated to the hot-wire measurement; the θs-spectrum exhibits a pronounced peak at the vortex shedding frequency, as identified in Eu, for each L/d. The results suggest that the FBG sensor can be used to measure reliably both time-averaged and fluctuating temperatures. The heat transfer characteristics of the heated cylinder are examined for different L/d and further compared with the case of an isolated cylinder.