Rapid polymerase chain reaction (PCR)-single-stranded conformational polymorphism (SSCP) screening method for the identification ofAspergillussectionNigrispecies by the detection of calmodulin nucleotide variations

2007 ◽  
Vol 24 (10) ◽  
pp. 1148-1153 ◽  
Author(s):  
A. Susca ◽  
G. Stea ◽  
G. Perrone
Keyword(s):  
Polymerase Chain Reaction ◽  
Screening Method ◽  
Chain Reaction ◽  
Conformational Polymorphism ◽  
Single Stranded Conformational Polymorphism ◽  
Rapid Polymerase Chain Reaction ◽  
Polymerase Chain

Variation in ovine KRTAP8-1 is associated with variation in wool fibre staple strength and curvature

2019 ◽  
Vol 157 (6) ◽  
pp. 550-554 ◽  
Author(s):  
H. Gong ◽  
H. Zhou ◽  
W. Li ◽  
J. Wang ◽  
S. Li ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Marker ◽  
Protein Gene ◽  
Wool Fibre ◽  
Polymorphism Analysis ◽  
Chain Reaction ◽  
Conformational Polymorphism ◽  
Single Stranded Conformational Polymorphism ◽  
Polymerase Chain ◽  
Staple Strength

AbstractKRTAP8-1 was the initial high-glycine-tyrosine keratin-associated protein gene recognized in sheep, but little is known about the functional influence of this gene. The current study used polymerase chain reaction-single stranded conformational polymorphism analysis to genotype KRTAP8-1 in 391 Southdown × Merino-cross sheep from six sire-lines. Five previously described variants (named A to E) of KRTAP8-1 were identified with frequencies of 67.0, 14.2, 7.0, 10.7 and 1.0%, respectively. Of the four variants (A, B, C and D) that occurred at a frequency greater than 5%, the presence of C was found to be associated with a reduction in mean fibre curvature (MFC) and the presence of D was associated with an increase in mean staple strength (MSS), whereas the presence of A had a trend of association with reduced MSS. Associations were not identified with other wool traits. These results suggest that variation in KRTAP8-1 affects MSS and MFC, and that KRTAP8-1 has the potential to be used as a genetic marker for improving these traits.

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