scholarly journals rpoB Gene Mutations in Rifampin-Resistant Mycobacterium tuberculosis Identified by Polymerase Chain Reaction Single-Stranded Conformational Polymorphism

2001 ◽  
Vol 7 (6) ◽  
pp. 1010-1013 ◽  
Author(s):  
Miriam Bobadilla-del-Valle
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Tuberculosis ◽  
Gene Mutations ◽  
Rpob Gene ◽  
Chain Reaction ◽  
Conformational Polymorphism ◽  
Single Stranded Conformational Polymorphism ◽  
Polymerase Chain

Variation in ovine KRTAP8-1 is associated with variation in wool fibre staple strength and curvature

2019 ◽  
Vol 157 (6) ◽  
pp. 550-554 ◽  
Author(s):  
H. Gong ◽  
H. Zhou ◽  
W. Li ◽  
J. Wang ◽  
S. Li ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Marker ◽  
Protein Gene ◽  
Wool Fibre ◽  
Polymorphism Analysis ◽  
Chain Reaction ◽  
Conformational Polymorphism ◽  
Single Stranded Conformational Polymorphism ◽  
Polymerase Chain ◽  
Staple Strength

AbstractKRTAP8-1 was the initial high-glycine-tyrosine keratin-associated protein gene recognized in sheep, but little is known about the functional influence of this gene. The current study used polymerase chain reaction-single stranded conformational polymorphism analysis to genotype KRTAP8-1 in 391 Southdown × Merino-cross sheep from six sire-lines. Five previously described variants (named A to E) of KRTAP8-1 were identified with frequencies of 67.0, 14.2, 7.0, 10.7 and 1.0%, respectively. Of the four variants (A, B, C and D) that occurred at a frequency greater than 5%, the presence of C was found to be associated with a reduction in mean fibre curvature (MFC) and the presence of D was associated with an increase in mean staple strength (MSS), whereas the presence of A had a trend of association with reduced MSS. Associations were not identified with other wool traits. These results suggest that variation in KRTAP8-1 affects MSS and MFC, and that KRTAP8-1 has the potential to be used as a genetic marker for improving these traits.

scholarly journals IDENTIFICATION GENE MUTATIONS RPOB CAUSE OF MULTIDRUG RESISTANCE TUBERCULOSIS IN HAJI ADAM MALIK HOSPITAL

2018 ◽  
Vol 11 (13) ◽  
pp. 155
Author(s):  
Adhisty Nurpermatasari ◽  
Urip Harahap ◽  
Parluhutan Siagian
Keyword(s):  
Polymerase Chain Reaction ◽  
Multidrug Resistance ◽  
General Pattern ◽  
Gene Mutations ◽  
Rpob Gene ◽  
Multidrug Resistant ◽  
Chain Reaction ◽  
Mdr Tb ◽  
Pcr Products ◽  
Polymerase Chain

Objective: The study aimed to investigate gene mutations in Mycobacterium tuberculosis causing resistance to rifampicin (RIF) in multidrug resistance TB patients in Haji Adam Malik Hospital and determined the suitability of the general pattern of mutations that occur in patients with multidrug-resistant TB (MDR-TB) in the hospital with global mutation pattern.Methods: This observational study was performed prospectively in 31 patients with MDR-TB who had data medical record results of GeneXpert MTB/ RIF positive for resistant to RIF period August–November 2016 in the hospital by taking sputum from patients. DNA extracted from sputum and then purified to be detected using polymerase chain reaction (PCR). PCR products were examined by electrophoresis.Results: The highest percentage of 81 bp rpoB gene mutation MTB is called as RIF -resistance determining region which was located at codon 516 (100%) and then was followed by mutation at codon 531 (96.77%) and codon 526 (90.32%), respectively, while the smallest percentage of mutation (12.90%) MTB at codon was 533.Conclusion: The rpoB gene shows a positive mutation-causing resistance to RIF.

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