The low temperature method for environmental scanning electron microscopy – a new method for observation of diatom assemblages in vivo

2018 ◽  
Vol 33 (3) ◽  
pp. 397-403 ◽  
Author(s):  
Markéta Fránková ◽  
Aloisie Poulíčková ◽  
Vilém Neděla ◽  
Eva Tihlaříková ◽  
Kateřina Šumberová ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Low Temperature ◽  
Environmental Scanning Electron Microscopy ◽  
New Method ◽  
Environmental Scanning ◽  
Diatom Assemblages ◽  
Temperature Method ◽  
Scanning Electron

Environmental scanning electron microscopy of microcomedones

1994 ◽  
Vol 52 ◽  
pp. 370-371
Author(s):  
K. Hoyberg ◽  
H. Knaggs
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Environmental Scanning Electron Microscopy ◽  
Depth Of Field ◽  
Natural State ◽  
Environmental Scanning ◽  
Differential Pumping ◽  
Scanning Electron

Environmental Scanning Electron Microscopy (ESEM) combines the best features of conventional SEM (high resolution, depth of field) with the flexibility and ease of light microscopy. This is accomplished by the incorporation of a four stage differential pumping system and the utilization of a gaseous detector. These modifications allow the direct observation of wet, dry, and nonconductive specimens without sample preparation.In vivo methods for assessing the effectiveness of acne therapies are time consuming and require recruiting, treating and monitoring patients for several weeks. ESEM is an ideal technique to study, in vitro, changes that occur in microcomedone structures during treatment with anti-acne agents. The samples are viewed in their natural state, and can therefore be viewed before and after treatment.Microcomedones extracted by cyanoacrylate biopsy technique were examined via ESEM Approximately 4-5 areas per sample were selected. These areas were chosen to include small and large microcomedones, with or without vellus hairs and or microorganisms. Images of each area were recorded by electronic imaging and the coordinates of the areas stored via the instrument's computer.

Environmental scanning electron microscopy of fresh human gallstones reveals new morphologies of precipitated calcium salts

1992 ◽  
Vol 50 (2) ◽  
pp. 1322-1323
Author(s):  
Howard S. Kaufman ◽  
Keith D. Lillemoe ◽  
John T. Mastovich ◽  
Henry A. Pitt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Environmental Scanning Electron Microscopy ◽  
Environmental Scanning ◽  
X Ray Diffraction ◽  
Calcium Salts ◽  
Cholesterol Gallstones ◽  
X Ray ◽  
Ca Carbonate ◽  
Scanning Electron

Gallstones contain precipitated cholesterol, calcium salts, and proteins. Calcium (Ca) bilirubinate, palmitate, phosphate, and carbonate occurring in gallstones have variable morphologies but characteristic windowless energy dispersive x-ray (EDX) spectra. Previous studies of gallstone microstructure and composition using scanning electron microscopy (SEM) with EDX have been limited to dehydrated samples. In this state, Ca bilirubinates appear as either glassy masses, which predominate in black pigment stones, or as clusters, which are found mostly in cholesterol gallstones. The three polymorphs of Ca carbonate, calcite, vaterite, and aragonite, have been identified in gallstones by x-ray diffraction, however; the morphologies of these crystals vary in the literature. The purpose of this experiment was to study fresh gallstones by environmental SEM (ESEM) to determine if dehydration affects gallstone Ca salt morphology.Gallstones and bile were obtained fresh at cholecystectomy from 6 patients. To prevent dehydration, stones were stored in bile at 37°C. All samples were studied within 4 days of procurement.

Characterization of Thin Polymer Blend Films using ESEM – No Charging, No Staining.

2001 ◽  
Vol 707 ◽  
Author(s):  
Ian C. Bache ◽  
Catherine M. Ramsdale ◽  
D. Steve Thomas ◽  
Ana-Claudia Arias ◽  
J. Devin MacKenzie ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Environmental Scanning Electron Microscopy ◽  
Environmental Scanning ◽  
Cross Sectional ◽  
Blend Films ◽  
Thin Polymer ◽  
Device Applications ◽  
Scanning Electron

ABSTRACTCharacterising the morphology of thin films for use in device applications requires the ability to study both the structure within the plane of the film, and also through its thickness. Environmental scanning electron microscopy has proved to be a fruitful technique for the study of such films both because contrast can be seen within the film without the need for staining (as is conventionally done for electron microscopy), and because cross-sectional images can be obtained without charging artefacts. The application of ESEM to a particular blend of relevance to photovoltaics is described.

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