Intervertebral disc organ culture for the investigation of disc pathology and regeneration – benefits, limitations, and future directions of bioreactors

2019 ◽  
Vol 61 (3-4) ◽  
pp. 304-321 ◽  
Author(s):  
Judith-Johanna Pfannkuche ◽  
Wei Guo ◽  
Shangbin Cui ◽  
Junxuan Ma ◽  
Gernot Lang ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Future Directions

scholarly journals Correction: Growing a backbone – functional biomaterials and structures for intervertebral disc (IVD) repair and regeneration: challenges, innovations, and future directions

2021 ◽  
Vol 9 (6) ◽  
pp. 2322-2323
Author(s):  
Matthew D. Harmon ◽  
Daisy M. Ramos ◽  
D. Nithyadevi ◽  
Rosalie Bordett ◽  
Swetha Rudraiah ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Future Directions ◽  
Functional Biomaterials

Correction for ‘Growing a backbone – functional biomaterials and structures for intervertebral disc (IVD) repair and regeneration: challenges, innovations, and future directions’ by Matthew D. Harmon et al., Biomater. Sci., 2020, 8, 1216–1239, DOI: 10.1039/C9BM01288E.

scholarly journals A Hyaluronan and Platelet-Rich Plasma Hydrogel for Mesenchymal Stem Cell Delivery in the Intervertebral Disc: An Organ Culture Study

2021 ◽  
Vol 22 (6) ◽  
pp. 2963
Author(s):  
Fabrizio Russo ◽  
Luca Ambrosio ◽  
Marianna Peroglio ◽  
Wei Guo ◽  
Sebastian Wangler ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intervertebral Disc ◽  
Organ Culture ◽  
Platelet Rich Plasma ◽  
Cell Activity ◽  
Human Mesenchymal Stem Cells ◽  
Cell Phenotype ◽  
Cytokeratin 19 ◽  
Stem Cell Delivery ◽  
Normal Metabolism

The purpose of the present pilot study was to evaluate the effect of a hydrogel composed of hyaluronic acid (HA) and platelet-rich plasma (PRP) as a carrier for human mesenchymal stem cells (hMSCs) for intervertebral disc (IVD) regeneration using a disc organ culture model. HA was mixed with batroxobin (BTX) and PRP to form a hydrogel encapsulating 1 × 106 or 2 × 106 hMSCs. Bovine IVDs were nucleotomized and filled with hMSCs suspended in ~200 μL of the PRP/HA/BTX hydrogel. IVDs collected at day 0 and nucleotomized IVDs with no hMSCs and/or hydrogel alone were used as controls. hMSCs encapsulated in the hydrogel were also cultured in well plates to evaluate the effect of the IVD environment on hMSCs. After 1 week, tissue structure, scaffold integration, hMSC viability and gene expression of matrix and nucleus pulposus (NP) cell markers were assessed. Histological analysis showed a better preservation of the viability of the IVD tissue adjacent to the gel in the presence of hMSCs (~70%) compared to the hydrogel without hMSCs. Furthermore, disc morphology was maintained, and the hydrogel showed signs of integration with the surrounding tissues. At the gene expression level, the hydrogel loaded with hMSCs preserved the normal metabolism of the tissue. The IVD environment promoted hMSC differentiation towards a NP cell phenotype by increasing cytokeratin-19 (KRT19) gene expression. This study demonstrated that the hydrogel composed of HA/PRP/BTX represents a valid carrier for hMSCs being able to maintain a good cell viability while stimulating cell activity and NP marker expression.

scholarly journals An organ culture system to model early degenerative changes of the intervertebral disc II: profiling global gene expression changes

2013 ◽  
Vol 15 (5) ◽  
pp. R121 ◽  
Author(s):  
Dessislava Z Markova ◽  
Christopher K Kepler ◽  
Sankar Addya ◽  
Hallie B Murray ◽  
Alexander R Vaccaro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Global Gene Expression ◽  
Degenerative Changes ◽  
Organ Culture System

scholarly journals Development of an intact intervertebral disc organ culture system in which degeneration can be induced as a prelude to studying repair potential

2011 ◽  
Vol 20 (8) ◽  
pp. 1244-1254 ◽  
Author(s):  
Bernice Jim ◽  
Thomas Steffen ◽  
Janet Moir ◽  
Peter Roughley ◽  
Lisbet Haglund
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Organ Culture System

Cryopreserved intervertebral disc with injected bone marrow–derived stromal cells: a feasibility study using organ culture

2010 ◽  
Vol 10 (6) ◽  
pp. 486-496 ◽  
Author(s):  
Samantha C.W. Chan ◽  
Benjamin Gantenbein-Ritter ◽  
Victor Y.L. Leung ◽  
Danny Chan ◽  
Kenneth M.C. Cheung ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Intervertebral Disc ◽  
Organ Culture ◽  
Feasibility Study ◽  
Stromal Cells

scholarly journals Effects of Asymmetric Dynamic Loading on Intervertebral Disc – towards a Scoliosis Mimicking Organ Culture Model

2016 ◽  
Vol 6 (1_suppl) ◽  
pp. s-0036-1582636-s-0036-1582636
Author(s):  
Zhen Li ◽  
Ying Zhang ◽  
Lukas Straumann ◽  
Patrick Lezuo ◽  
Marianna Peroglio ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Dynamic Loading ◽  
Culture Model

scholarly journals Compression Loading Induced Cellular Stress Response of Intervertebral Disc Cells in Organ Culture

2016 ◽  
Vol 6 (1_suppl) ◽  
pp. s-0036-1582604-s-0036-1582604
Author(s):  
Wai Hon Chooi ◽  
Samantha C. W. Chan ◽  
Benjamin Gantenbein ◽  
Barbara P. Chan
Keyword(s):  
Stress Response ◽  
Intervertebral Disc ◽  
Organ Culture ◽  
Cellular Stress ◽  
Cellular Stress Response ◽  
Compression Loading ◽  
Disc Cells

Form and Function of the intervertebral Disc: Human Organ Culture and Histological Stain Comparison Study

2014 ◽  
Vol 4 (1_suppl) ◽  
pp. s-0034-1376615-s-0034-1376615
Author(s):  
B. A Walter ◽  
O. Marcial Torre ◽  
S. Illien-Junger ◽  
D. M. Laudier ◽  
J. C Iatridis
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Comparison Study ◽  
Human Organ ◽  
Form And Function ◽  
And Function

Effects of Caffeine on Intervertebral Disc Cell Viability in a Whole Organ Culture Model

2017 ◽  
Vol 17 (10) ◽  
pp. S125-S126
Author(s):  
Benjamin T. Raines ◽  
James T. Stannard ◽  
Olivia E. Stricklin ◽  
Aaron M. Stoker ◽  
Theodore J. Choma ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Cell Viability ◽  
Disc Cell ◽  
Culture Model
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