Polymerase chain reaction and nucleic acid spot hybridisation detection of begomovirus(es) associated with apical leaf curl disease of potato

2011 ◽  
Vol 44 (10) ◽  
pp. 987-992 ◽  
Author(s):  
Ellapalayam Palanisamy Venkatasalam ◽  
Sarjeet Singh ◽  
Palaiyur Nanjappan Sivalingam ◽  
Varagur Ganeshan Malathi ◽  
Ishwar Das Garg
2019 ◽  
Vol 11 (43) ◽  
pp. 5568-5576
Author(s):  
Sonja Elf ◽  
Kevin E. Eboigbodin

SIBA is an established nucleic acid amplification method that is used as an alternative to polymerase chain reaction (PCR).


2019 ◽  
Vol 65 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Arthur Elia Mongan ◽  
Josef Sem Berth Tuda ◽  
Lucky Ronald Runtuwene

Abstract Infectious disease is still a major threat in the world today. Five decades ago, it was considered soon to be eradicated, but the adaptation of pathogens to environmental pressure, such as antimicrobials, encouraged the emergence and reemergence of infectious disease. The fight with infectious disease starts with prevention, diagnosis, and treatment. Diagnosis can be upheld by observing the cause of disease under the microscope or detecting the presence of nucleic acid and proteins of the pathogens. The molecular techniques span from classical polymerase chain reaction (PCR) to sequencing the nucleic acid composition. Here, we are reviewing the works have been undertaken to utilize a portable sequencer, MinION, in various aspects of infectious disease management.


Blood ◽  
1991 ◽  
Vol 77 (4) ◽  
pp. 879-886 ◽  
Author(s):  
FJ Sunzeri ◽  
TH Lee ◽  
RG Brownlee ◽  
MP Busch

Abstract The polymerase chain reaction (PCR) technique is a powerful new tool for amplifying target DNA, thus allowing for sensitive detection of specific nucleic acid sequences. One important potential use of PCR involves screening the donated blood supply for transfusion-transmitted viruses. Realization of this goal has been limited by (1) the requirement for multiple, discrete PCR reactions to amplify and detect target sequences of more than one virus, and (2) the lack of a rapid, nonhazardous means for specific detection of one or more PCR-amplified products. We report the simultaneous amplification of three distinct target sequences without discernable loss in sensitivity toward any single target sequence. We also demonstrate very rapid separation and detection of PCR-amplified viral DNA through the use of automated capillary DNA chromatography. Amplified DNA peaks were initially identified by scanning the capillary effluent at ultraviolet wavelengths, while discrimination of human immunodeficiency virus type 1 and human T-cell leukemic virus type I PCR-amplified DNA was accomplished through use of virus-specific, fluorescently labeled primers and probes. These results indicate progress toward an automated system for screening the blood supply for nucleic acid sequences of multiple pathogens.


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